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Recognition of gram-negative and gram-positive bacteria with a functionalized conducting polymer film

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Abstract

In this work, we successfully developed bacterial templates on the surface of an overoxidized polypyrrole film using both gram-negative and gram-positive bacteria in which bacterial surface chemical structures are precisely transferred at a molecular level. The sensor film identified target bacteria within minutes through a unique combination with dielectrophoresis. The bacterial cavities had high selectivity for distinguishing specific target bacteria in bacterial mixtures containing gram-negative (Escherichia coli and Pseudomonas aeruginosa) and gram-positive (Bacillus subtilis and Staphylococcus aureus) bacteria. This rapid and specific recognition system will enable not only bacterial sensing but also analysis of various biological species.

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References

  1. J.L. Bredas, G.B. Street, Acc. Chem. Res. 18, 10 (1985)

    Article  Google Scholar 

  2. C. Malitesta, E. Mazzotta, R.A. Picca, A. Poma, I. Chianella, S.A. Piletsky, Anal. Bioanal. Chem. 402, 5 (2012)

    Article  Google Scholar 

  3. S. Tokonami, H. Shiigi, T. Nagaoka, Molecularly Imprinted Sensors, (Elsevier B.V., Amsterdam, The Netherlands, 2012), p. 74

  4. M. Heitzmann, C. Bucher, J.C. Moutet, E. Pereira, B.L. Rivas, G. Royal, E. Saint-Aman, Electrochim. Acta 52, 9 (2007)

    Article  Google Scholar 

  5. G.O. Buica, C. Bucher, J.C. Moutet, G. Royal, E. Saint-Aman, E.M. Ungureanu, Electroanalysis 21, 1 (2009)

    Article  Google Scholar 

  6. L.D. Spurlock, A. Jaramillo, A. Praserthdam, J. Lewis, A. BrajterToth, Anal. Chim. Acta 336, 1–3 (1996)

    Article  Google Scholar 

  7. S. Takeda, H. Yagi, S. Mizuguchi, H. Funahashi, H. Shiigi, T. Nagaoka, J. Flow Inject. Anal. 25, 1 (2008)

    Google Scholar 

  8. B. Deore, Z.D. Chen, T. Nagaoka, Anal. Chem. 72, 17 (2000)

    Article  Google Scholar 

  9. M. Labib, A.S. Zamay, O.S. Koloyskaya, I.T. Reshetneva, G.S. Zamay, R.J. Kibbee, S.A. Sattar, T.N. Zamay, M.V. Berezovski, Anal. Chem. 84, 21 (2012)

    Article  Google Scholar 

  10. P. Daly, T. Collier, S. Doyle, Lett. Appl. Microbiol. 34, 3 (2002)

    Article  Google Scholar 

  11. A. Wensing, M. Gernold, K. Geider, J. Appl. Microbiol. 112, 1 (2012)

    Article  Google Scholar 

  12. N. Yamaguchi, M. Sasada, M. Yamanaka, M. Nasu, Cytom Part A 54A, 1 (2003)

    Article  Google Scholar 

  13. T. Satoh, Y. Shinoda, M. Alexandrov, A. Kuroda, Y. Murakami, Anal. Biochem. 379, 1 (2008)

    Article  Google Scholar 

  14. J. Wang, J.Q. Gao, D.J. Liu, D.X. Han, Z.X. Wang, Nanoscale 4, 2 (2012)

    Article  Google Scholar 

  15. S. Tokonami, K. Saimatsu, Y. Nakadoi, M. Furuta, H. Shiigi, T. Nagaoka, Anal. Sci. 28, 4 (2012)

    Article  Google Scholar 

  16. S. Tokonami, Y. Nakadoi, M. Takahashi, M. Ikemizu, T. Kadoma, K. Saimatsu, L.Q. Dung, H. Shiigi, T. Nagaoka, Anal. Chem. 85, 10 (2013)

    Article  Google Scholar 

  17. W.W. Wilson, M.M. Wade, S.C. Holman, F.R. Champlin, J. Microbiol. Methods 43, 3 (2001)

    Article  Google Scholar 

  18. J.J. Shephard, D.M. Savory, P.J. Bremer, A.J. McQuillan, Langmuir 26, 11 (2010)

    Google Scholar 

  19. V.B. Tran, Y.S. Sung, S.M.J. Fleiszig, D.J. Evans, C.J. Radke, J. Colloid Interf. Sci. 362, 1 (2011)

    Article  Google Scholar 

  20. B. Deore, Z.D. Chen, T. Nagaoka, Anal. Sci. 15, 9 (1999)

    Article  Google Scholar 

  21. H. Shiigi, K. Okamura, D. Kijima, B. Deore, U. Sree, T. Nagaoka, J. Electrochem. Soc. 150, 5 (2003)

    Article  Google Scholar 

  22. H. Shiigi, D. Kijima, Y. Ikenaga, K. Hori, S. Fukazawa, T. Nagaoka, J. Electrochem. Soc. 152, 8 (2005)

    Google Scholar 

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Acknowledgments

We thank Prof. Yamazaki for arranging the use of the experimental facilities. We thank Dr. Okajima for assistance with bacterial cultivation. This work was partially supported by Special Coordination Funds for Promoting Scientific and Technology (SCF) of MEXT. We gratefully acknowledge the financial support provided by the Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (A) (grant 24685013), and the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries, and Food Industry (grant 25020A).

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Authors and Affiliations

  1. Nanoscience and Nanotechnology Research Center, Osaka Prefecture University, 1-2, Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8570, Japan

    Shiho Tokonami, Yu Nakadoi & Hiroyuki Nakata

  2. Graduate School of Engineering, Osaka Prefecture University, 1-1, Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, Japan

    Yu Nakadoi, Hiroyuki Nakata, Hiroshi Shiigi & Tsutomu Nagaoka

  3. Sharp Corporation, 3-1-72, Kitakamei-cho, Yao, 581-8585, Japan

    Seiji Takami & Tetsuya Kadoma

Authors
  1. Shiho Tokonami
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  2. Yu Nakadoi
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  3. Hiroyuki Nakata
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  4. Seiji Takami
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  5. Tetsuya Kadoma
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  6. Hiroshi Shiigi
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  7. Tsutomu Nagaoka
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Correspondence to Shiho Tokonami.

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Tokonami, S., Nakadoi, Y., Nakata, H. et al. Recognition of gram-negative and gram-positive bacteria with a functionalized conducting polymer film. Res Chem Intermed 40, 2327–2335 (2014). https://doi.org/10.1007/s11164-014-1609-6

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  • DOI: https://doi.org/10.1007/s11164-014-1609-6

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