Abstract
Nowadays, a growing body of evidence supports the view that plants offer an extraordinary opportunity to discover and develop new promising therapeutic strategies for many diseases, including cancer. Here we tested the anticancer action against Hepatocellular carcinoma (HCC) of extracts obtained from two plants harvested in Apulia, namely Brassica oleracea L. and Crithmum maritimum L. B. oleracea was grown in biodynamical agriculture without any agrochemical input, instead C. maritimum was collected on Apulian coasts and is still commonly eaten in Apulia. HCC, one of the most frequent tumors worldwide, is estimated to become the third leading cause of cancer-related deaths in Western Countries by 2030. The approved synthetic drugs for the treatment of HCC are currently inadequate in terms of therapeutic results and tolerability. Hence, aim of the present study was to test the anticancer action against HCC of extracts obtained from Brassica oleracea L. and Crithmum maritimum L. We preliminary prepared extracts from both plants using four solvents with different polarity: hexane, ethyl acetate, methanol and ethanol. Then, we tested the effect of the different fractions in inhibiting HCC cell growth. Finally, we characterized the mechanism of action of the most effective fraction. We found that ethyl acetate fractions from both plants were the most effective in inhibiting HCC growth. In particular, we demonstrated that these fractions effectively reduce HCC growth by exerting, on one hand, a cytostatic effect through their action on the cell cycle, and on the other hand by triggering apoptosis and necrosis. Our findings support the notion that ethyl acetate fractions from Apulian B. oleracea and C. maritimum can be in perspective considered as promising tools to expand the opportunities to identify new and not toxic anticancer therapeutic approaches for HCC. Further pharmacological investigations will shed light on how this could be effectively achieved.
Similar content being viewed by others
Abbreviations
- ANOVA:
-
one-way analysis of variance
- DMEM:
-
Dulbecco’s modified Eagle’s medium
- DMSO:
-
dimethyl sulfoxide
- EtOH:
-
ethanol
- FBS:
-
fetal bovine serum
- HCC:
-
hepatocellular carcinoma
- HPLC:
-
high performance liquid chromatography
- MetS:
-
metabolic syndrome
- MTT:
-
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
- NAFLD:
-
non-alcoholic fatty liver disease
- PBS:
-
phosphate buffered saline
- SRB:
-
sulforhodamine B
References
Butler MS, Newman DJ (2008) Mother nature’s gifts to diseases of man: the impact of natural products on anti-infective, anticholestemics and anticancer drug discovery. Prog Drug Res 65(1):3–44
Newman DJ (2008) Natural products as leads to potential drugs: an old process or the new hope for drug discovery? J Med Chem 51(9):2589–2599. https://doi.org/10.1021/jm0704090
Coseri S (2009) Natural products and their analogues as efficient anticancer drugs. Mini-Rev Med Chem 9(5):560–571
de Lope CR, Tremosini S, Forner A, Reig M, Bruix J (2012) Management of HCC. J Hepatol 56(Suppl 1):S75–S87. https://doi.org/10.1016/S0168-8278(12)60009-9
Forner A, Reig M, Bruix J (2018) Hepatocellular carcinoma. Lancet. https://doi.org/10.1016/S0140-6736(18)30010-2
Kudo M (2018) Systemic Therapy for hepatocellular carcinoma: latest advances. Cancers (Basel) 10(11):E412. https://doi.org/10.3390/cancers10110412
Waidmann O (2018) Recent Developments with immunotherapy for hepatocellular carcinoma. Expert Opin Biol Ther 18(8):905–910. https://doi.org/10.1080/14712598.2018.1499722
Wu Z, Lai L, Li M, Zhang L, Zhang W (2017) Acute liver failure caused by pembrolizumab in a patient with pulmonary metastatic liver cancer: a case report. Medicine (Baltimore) 96(51):e9431. https://doi.org/10.1097/MD.0000000000009431
Beecher CW (1994) Cancer preventive properties of varieties of Brassica oleracea: a review. Am J Clin Nutr 59(5 Suppl):1166S–1170S. https://doi.org/10.1093/ajcn/59.5.1166S
Murillo G, Mehta RG (2001) Cruciferous vegetables and cancer prevention. Nutr Cancer 41(1–2):17–28. https://doi.org/10.1080/01635581.2001.9680607
Talalay P, Fahey JW (2001) Phytochemicals from cruciferous plants protect against cancer by modulating carcinogen metabolism. J Nutr 131(11 Suppl):3027S–3033S. https://doi.org/10.1093/jn/131.11.3027S
Novio S, Cartea ME, Soengas P, Freire-Garabal M, Nunez-Iglesias MJ (2016) Effects of brassicaceae isothiocyanates on prostate cancer. Molecules 21(5):E626. https://doi.org/10.3390/molecules21050626
Pappa G, Lichtenberg M, Iori R, Barillari J, Bartsch H, Gerhauser C (2006) Comparison of growth inhibition profiles and mechanisms of apoptosis induction in human colon cancer cell lines by isothiocyanates and indoles from brassicaceae. Mutat Res 599(1–2):76–87. https://doi.org/10.1016/j.mrfmmm.2006.01.007
Renna M (2018) Reviewing the prospects of sea fennel (Crithmum maritimum L.) as emerging vegetable crop. Plants (Basel) 7(4):E92. https://doi.org/10.3390/plants7040092
Coiffard L, Piron-Frenet M, Amicel L (1993) Geographical variations of the constituents of the essential oil of Crithmum maritimum L., apiaceae. Int J Cosmet Sci 15(1):15–21. https://doi.org/10.1111/j.1467-2494.1993.tb00064.x
Meot-Duros L, Magne C (2009) Antioxidant activity and phenol content of Crithmum maritimum L. leaves. Plant Physiol Biochem 47(1):37–41. https://doi.org/10.1016/j.plaphy.2008.09.006
Ruberto G, Baratta MT, Deans SG, Dorman HJ (2000) Antioxidant and antimicrobial activity of Foeniculum vulgare and Crithmum maritimum essential oils. Planta Med 66(8):687–693. https://doi.org/10.1055/s-2000-9773
Gerl R, Vaux DL (2005) Apoptosis in the development and treatment of cancer. Carcinogenesis 26(2):263–270. https://doi.org/10.1093/carcin/bgh283
Kang MR, Kim HM, Kang JS, Lee K, Lee SD, Hyun DH, In MJ, Park SK, Kim DC (2011) Lipid-soluble ginseng extract induces apoptosis and G0/G1 cell cycle arrest in NCI-H460 human lung cancer cells. Plant Foods Hum Nutr 66(2):101–106. https://doi.org/10.1007/s11130-011-0232-6
Kumar G, Gupta R, Sharan S, Roy P, Pandey DM (2019) Anticancer activity of plant veaves extract collected from a Tribal region of India. 3 Biotech 9(11):399. https://doi.org/10.1007/s13205-019-1927-x
Jung S, Shin J, Oh J, Enkhtaivan G, Lee SW, Gopal J, Sydara K, Saini RK, Keum YS, Oh JW (2019) Cytotoxic and apoptotic potential of Phyllodium elegans extracts on human cancer cell lines. Bioengineered. https://doi.org/10.1080/21655979.2019.1682110
Gordaliza M (2007) Natural products as leads to anticancer drugs. Clin Transl Oncol 9(12):767–776
Saklani A, Kutty SK (2008) Plant-derived compounds in clinical trials. Drug Discov Today 13(3–4):161–171. https://doi.org/10.1016/j.drudis.2007.10.010
Karna P, Chagani S, Gundala SR, Rida PC, Asif G, Sharma V, Gupta MV, Aneja R (2012) Benefits of whole ginger extract in prostate cancer. Br J Nutr 107(4):473–484. https://doi.org/10.1017/S0007114511003308
Liu RH (2003) Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am J Clin Nutr 78(3 Suppl):517S–520S. https://doi.org/10.1093/ajcn/78.3.517S
Thoppil RJ, Harlev E, Mandal A, Nevo E, Bishayee A (2013) Antitumor activities of extracts from selected desert plants against HepG2 human hepatocellular carcinoma cells. Pharm Biol 51(5):668–674. https://doi.org/10.3109/13880209.2012.749922
Ma JF, Wei PF, Guo C, Shi YP, Lv Y, Qiu LX, Wen LP (2018) The ethyl acetate extract of Gynura formosana Kitam. leaves inhibited cervical cancer cell proliferation via induction of autophagy. Biomed Res Int 2018:4780612. https://doi.org/10.1155/2018/4780612
Cao Z, Lin W, Huang Z, Chen X, Zhao J, Zheng L, Ye H, Liu Z, Liao L, Du J (2013) Ethyl acetate extraction from a Chinese herbal formula, Jiedu Xiaozheng Yin, inhibits the proliferation of hepatocellular carcinoma cells via induction of G0/G1 phase arrest in vivo and in vitro. Int J Oncol 42(1):202–210. https://doi.org/10.3892/ijo.2012.1703
Chen XZ, Cao ZY, Li JN, Hu HX, Zhang YQ, Huang YM, Liu ZZ, Hu D, Liao LM, Du J (2014) Ethyl acetate extract from Jiedu Xiaozheng Yin inhibits the proliferation of human hepatocellular carcinoma cells by suppressing polycomb gene product Bmi1 and Wnt/beta-catenin signaling. Oncol Rep 32(6):2710–2718. https://doi.org/10.3892/or.2014.3541
Acknowledgements
We are grateful to Ms. Loredana Acquaro for her precious technical support and to Dr. Marco Troisi for his technical support with the high-performance liquid chromatographic analysis. We are also in debt with Mr. Vito Moretti, who kindly provided us with B. oleracea, which was harvested in his biodynamic agricultural farm “La Calamita Rosa” (Castellaneta, Taranto, Italy).
Funding
This work was supported by Agenzia Regionale Strategica per la Salute ed. il Sociale – ARESS Puglia – in accordance with the project “The Apulian Lifestyle” – Delibera di Giunta n. 556 (18/04/2017).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of Interest
The authors have no conflicts of interest to declare.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic Supplementary Material
ESM 1
(PDF 3045 kb)
Rights and permissions
About this article
Cite this article
Gnocchi, D., Cesari, G., Calabrese, G.J. et al. Inhibition of Hepatocellular Carcinoma Growth by Ethyl Acetate Extracts of Apulian Brassica oleracea L. and Crithmum maritimum L.. Plant Foods Hum Nutr 75, 33–40 (2020). https://doi.org/10.1007/s11130-019-00781-3
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11130-019-00781-3