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Evaluation of Total Flavonoids, Myricetin, and Quercetin from Hovenia dulcis Thunb. As Inhibitors of α-Amylase and α-Glucosidase

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Abstract

This study was designed to investigate the inhibition effect and mechanism of total flavonoids, myricetin and quercetin extracted from Hovenia dulcis Thunb. on α-amylase and α-glucosidase in order to explore the potential use of Hovenia flavonoids in alleviating postprandial hyperglycemia. The results demonstrate that total flavonoids, myricetin, and quercetin were effective inhibitors of α-amylase with IC50 values of 32.8, 662 and 770 μg ml−1, respectively. And all three were effective inhibitors of α-glucosidase with IC50 values of 8, 3 and 32 μg ml−1, respectively. Enzyme kinetics tests and Lineweaver-Burk results showed the inhibition effects of total flavonoids, myricetin and quercrtin on α-amylase were all reversible and competitive, and the effects on α-glucosidase were all reversible but non-competitive. This study revealed that Hovenia flavonoids, especially myricetin, are effective and promising functional foods in alleviating type 2 diabetes mellitus.

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Abbreviations

ESI-MS:

The electrospray ionization mass spectrometry

H. dulcis Thunb.:

Hovenia dulcis Thunb.

NMR:

Nuclear magnetic resonance

T2DM:

Type 2 diabetes mellitus

TFs:

Total flavonoids

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Acknowledgments

This research was supported by the overall planning of Shaanxi science and technology (Project No. 2015KTCQ02-07), Doctoral Scientific Fund Project of the Ministry of Education of China (20130202120007), National Key Technology R&D Program (2015BAD16B02) and an earmarked fund for the China Agriculture Research System (CARS-28).

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Authors and Affiliations

  1. Shaanxi Engineering Lab for Food Green Processing and Security Control, College of Food Engineering and Nutritional Science, Shaanxi Normal University, 620 West Chang’an Avenue, Chang’an, Xi’an, 710119, People’s Republic of China

    Yonghong Meng, Anping Su, Shuang Yuan, Siyuan Tan, Hong Deng & Yurong Guo

  2. Xi’an Healthful Biotechnology Co., Ltd., HangTuo Road, Chang’an, Xi’an, 710100, People’s Republic of China

    Huaguo Zhao

  3. Department of Human Nutrition, Food and Animal Sciences, College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa, 1955 East West Road, AgSci 216, Honolulu, HI, 96822, USA

    Chingyuan Hu

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  1. Yonghong Meng
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  2. Anping Su
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  3. Shuang Yuan
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  4. Huaguo Zhao
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  7. Hong Deng
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  8. Yurong Guo
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Correspondence to Yonghong Meng.

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Meng, Y., Su, A., Yuan, S. et al. Evaluation of Total Flavonoids, Myricetin, and Quercetin from Hovenia dulcis Thunb. As Inhibitors of α-Amylase and α-Glucosidase. Plant Foods Hum Nutr 71, 444–449 (2016). https://doi.org/10.1007/s11130-016-0581-2

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