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Structure Identification of Triterpene from the Mushroom Pleurotus eryngii with Inhibitory Effects Against Breast Cancer

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Abstract

Breast cancer is the leading cause of death among women, with approximately 1 million diagnoses annually. Triterpenoids, which have cancer preventive or anti-tumour efficacy towards various tumour cells, may play a role in breast cancer prevention. In our previous study, an acetic ether (EtOAc) fraction from the sporocarp of the edible mushroom Pleurotus eryngii (P. eryngii) exhibited significant tumour cell growth inhibition both in vitro and in vivo. In this study, three pentacyclic triterpenoid compounds (1–3) were isolated from EtOAc extracts using chromatographic separation and were identified using nuclear magnetic resonance (NMR) and mass spectrometry (MS). The compounds were 2, 3, 6, 23-tetrahydroxy-urs-12-en-28 oic acid (1), 2,3,23-trihydroxy-urs-12-en-28 oic acid (2) and lupeol (3). All three purified triterpenes showed significant inhibitory activity against breast cancer MCF-7 cell lines in vitro, with the greatest activity exhibited by compound 1, followed by compound 2 and 3. The IC50 values were 15.71, 48 and 66.89 μM, respectively. Our study may help elucidate the health benefits of P. eryngii mushroom consumption.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No.31271979) and the Natural Science Foundation of Tianjin (No.15JCYBJC30100).

This article does not contain any studies with human or animal subjects.

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We have no conflict of interest.

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Authors and Affiliations

  1. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China

    Zhaohui Xue, Jiaomei Li, Aiqing Cheng, Xiaohong Kou & Fengjuan Zhou

  2. Tianjin Research Center of Agricultural Biotechnology, Tianjin, 300381, China

    Wancong Yu & Zhijun Zhang

Authors
  1. Zhaohui Xue
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  2. Jiaomei Li
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  3. Aiqing Cheng
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  4. Wancong Yu
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  5. Zhijun Zhang
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  6. Xiaohong Kou
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  7. Fengjuan Zhou
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Correspondence to Xiaohong Kou or Fengjuan Zhou.

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Xue, Z., Li, J., Cheng, A. et al. Structure Identification of Triterpene from the Mushroom Pleurotus eryngii with Inhibitory Effects Against Breast Cancer. Plant Foods Hum Nutr 70, 291–296 (2015). https://doi.org/10.1007/s11130-015-0492-7

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  • DOI: https://doi.org/10.1007/s11130-015-0492-7

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