Abstract
Açai, fruit from Euterpe oleraceae Martius, is consumed in natura and in a variety of beverages and food preparations and possesses several potential antioxidant compounds. In a first study for anticarcinogenicity screening, male Swiss mice (n = 20/per group) were chemically-induced to urothelial bladder carcinogenesis for 10 weeks and received a standard diet or a standard diet containing 2.5 and 5 % spray-dried açai pulp (AP) for 10 weeks. At week 20, the incidence of simple and nodular hyperplasia and the incidence and multiplicity of transitional cell carcinoma (TCC) were evaluated. In a second study for antigenotoxicity screening, male Swiss mice (n = 6/per group) were fed standard diet or standard diet containing 5 % AP for three weeks. Urothelial cell suspensions were obtained and challenged with H2O2 for induction of DNA damage and analyzed by comet assay. Overall, dietary 5 % AP reduced TCC incidence and multiplicity (p = 0.019 and p = 0.015, respectively) and tumor cell proliferation and p63 expression (p = 0.02 and p = 0.007, respectively), Furthermore, the group fed the 5 % AP presented a significant reduction (p < 0.01) in DNA damage induced by H2O2, a notable oxidant agent. The results suggest that the spray-dried açai pulp used here inhibits the TCC development in male Swiss mice, probably due to its potential antioxidant action.
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Acknowledgments
This study was supported by CAPES. Mariana F. Fragoso and Monize G do Prado and Luis F. Barbisan were recipients of fellowships from CAPES, FAPESP (07/54858-5, 09/50890-7) or CNPq (301585/2009-1), respectively.
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Supplementary Table
General and biochemical parameters evaluated in carcinogens-treated groups.1 (DOC 42 kb)
Supplementary Figure
Histopathology of urothelium detected in the HEstained sections. A) Control group (standard diet) and group receiving 0.5% AP showing non-altered urothelium (20x objective), B-E) BBN/MNU-treated groups showing simple and nodular hyperplasia (B-C, 20x objective) and TCC showing invasion on muscularis (D, detailed in E). (PPT 24718 kb)
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Fragoso, M.F., Prado, M.G., Barbosa, L. et al. Inhibition of Mouse Urinary Bladder Carcinogenesis by Açai Fruit (Euterpe oleraceae Martius) Intake. Plant Foods Hum Nutr 67, 235–241 (2012). https://doi.org/10.1007/s11130-012-0308-y
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DOI: https://doi.org/10.1007/s11130-012-0308-y