Abstract
Substantial evidence exists to support the hypothesis that high fruit and vegetable consumption, rich in antioxidants, can reduce the incidence of several disease states. The aim of this study was to compare the results obtained by six spectrophotometric biochemical methods including the ferric reducing antioxidant power (FRAP), 2, 2-diphenyl-1-picryhydrazyl (DPPH•), 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+), copper (II) reducing capacity (CUPRAC) and Cerium (IV) reducing antioxidant capacity (CERAC) assays as well as Folin-Ciocalteu method (FC) for the measurement of total antioxidant capacity (TAC) and total polyphenols (TP) in different commercially available vegetable juices. There was a significant positive correlation between the results obtained for FRAP, ABTS•+, CUPRAC, CERAC and FC (0.68 ≤ r ≤ 0.96, P < 0.01). DPPH• was only correlated with CERAC (r = 0.66, P < 0.01). Beetroot juice had the highest TAC and TP regardless of the method of analysis.
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Abbreviations
- ABTS:
-
2,2-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid
- CERAC:
-
Cerium IV reducing antioxidant capacity
- CUPRAC:
-
Copper II reducing antioxidant capacity
- DPPH:
-
2 2-diphenyl-1-picryhydrazyl
- FAE:
-
Ferulic acid equivalents
- FC:
-
Folin-Ciocalteu method
- FRAP:
-
Ferric reducing antioxidant power
- HAT:
-
Hydrogen atom transfer
- RONS:
-
Reactive oxygen and nitrogen species
- SET:
-
Single electron transfer
- TAC:
-
Total antioxidant capacity
- TE:
-
Trolox equivalents
- TP:
-
Total polyphenols
- Trolox:
-
6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid
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Acknowledgments
The authors wish to acknowledge the support of Professor David Fell during this study. The mention of product or brand names in this article are for the purposes of giving specific information only and do not imply any endorsements or recommendations by the Functional Food Centre, Oxford Brookes University. The authors report no conflict of interest, financial or otherwise.
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Wootton-Beard, P.C., Ryan, L. Combined use of Multiple Methodologies for the Measurement of Total Antioxidant Capacity in UK Commercially Available Vegetable Juices. Plant Foods Hum Nutr 67, 142–147 (2012). https://doi.org/10.1007/s11130-012-0287-z
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DOI: https://doi.org/10.1007/s11130-012-0287-z