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Effects of the Consumption of Caffeinated and Decaffeinated Instant Coffee Beverages on Oxidative Stress Induced by Strenuous Exercise in Rats

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Abstract

Many authors attribute the antioxidant activity of brewed coffee to its caffeine content. In addition, caffeine intake has been associated with increased performance during physical exercise. This study analyzed the in vivo effects of drinking caffeinated and decaffeinated instant coffee (8%, w/v) on oxidative stress and antioxidant enzyme activity in the anterior tibialis muscles of rats subjected to intense exercise. It was observed that exercise induced lipid peroxidation (estimated using malondialdehyde) and protein oxidation (evaluated by determining the formation of carbonyl groups) in the muscle (P < 0.05). Decaffeinated instant coffee and caffeine solution did not exhibit antioxidant activity in vivo. Caffeinated instant coffee beverage intake did not induce changes in superoxide dismutase and glutathione peroxidase activities but was able to diminish lipid and protein oxidation in the anterior tibialis muscles of rats after exercise (P < 0.05), contributing to a reduction in the oxidative stress triggered by exercise.

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Abbreviations

ANOVA:

Analysis of variance

CC:

Rats treated with caffeinated instant coffee and not subjected to intense exercise

CD:

Rats treated with decaffeinated instant coffee and not subjected to intense exercise

CF:

Rats treated with caffeine solution and not subjected to intense exercise

CK:

Serum creatine kinase

CNPq:

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Exer:

Rats subjected to intense exercise and treated with the vehicle (water)

Exer.CC:

Rats treated with caffeinated instant coffee and subjected to intense exercise

Exer.CD:

Rats treated with decaffeinated instant coffee and subjected to intense exercise

Exer.CF:

Rats treated with caffeine solution and subjected to intense exercise

Fapemig:

Fundação de Amparo à Pesquisa do Estado de Minas Gerais

FES:

Functional electrical stimulations

GPx:

Glutathione peroxidase

SOD:

Superoxide dismutase

TBARS:

Thiobarbituric acid-reactive substances

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Acknowledgements

The authors thank the FAPEMIG and CNPq for scientific initiation scholarships.

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Authors and Affiliations

  1. Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Rua Gabriel Monteiro da Silva, 700. Centro, Alfenas, MG, Brazil

    André Luiz Machado Viana, Miriam das Dores Mendes Fonseca, Elisson Lamin Jerônimo Meireles, Stella Maris da Silveira Duarte, Maria Rita Rodrigues & Fernanda Borges de Araujo Paula

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  1. André Luiz Machado Viana
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  2. Miriam das Dores Mendes Fonseca
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  6. Fernanda Borges de Araujo Paula
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Correspondence to Fernanda Borges de Araujo Paula.

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Viana, A.L.M., Fonseca, M.D.M., Meireles, E.L.J. et al. Effects of the Consumption of Caffeinated and Decaffeinated Instant Coffee Beverages on Oxidative Stress Induced by Strenuous Exercise in Rats. Plant Foods Hum Nutr 67, 82–87 (2012). https://doi.org/10.1007/s11130-011-0267-8

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