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Effect of Germinated Soybean Protein Hydrolysates on Adipogenesis and Adipolysis in 3T3-L1 Cells

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Abstract

Germination of soybeans increases the bioavailability of some nutrients. An evaluation was done to determine if germination increased the anti-adipogenic and lipolytic effects of soybean. Soybeans were germinated for 0 to 6 days and protein concentrates extracted from beans germinated at each period. Soy protein concentrates can retain notable amounts of phytochemicals with anti-adipogenic activity. For this reason, it was evaluated the effect of protein hydrolysates with and without phytochemicals in the adipocyte-like cells after 3T3-L1 (murine fibroblasts) cell line differentiation. Cell viability decreased with exposure to the germinated soybean protein hydrolysates during the differentiation stage, but not during the fibroblast or mature adipocyte stages. Adipogenesis and triglycerides accumulation were strongly inhibited by the hydrolysate from soybeans germinated for 2 days (with ethanol-soluble phytochemicals), when compared to ungerminated soybean. Adipolysis increased with exposure to hydrolysates from beans germinated for 2 days (with phytochemicals) and 5 days (without phytochemicals). Germinated soy protein hydrolysates had an effect on inhibition of lipid storage in adypocites and increasing lipolysis, which was improved by changes of the protein and increased phytochemical content due to germination.

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Authors and Affiliations

  1. I.P.N. Departamento de Graduados en Alimentos, Campus Sto. Tomás, Escuela Nacional de Ciencias Biológicas, Carpio y Plan de Ayala S/N, Col. Sto. Tomás, 11340, México, D.F., Mexico

    L. A. González-Espinosa de los Monteros & R. Mora-Escobedo

  2. I.P.N. Departamento de Morfología, Campus Zacatenco, Unidad Profesional “Adolfo López Mateos”, 07738, México, D.F., Mexico

    E. Ramón-Gallegos

  3. Departamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga No. 15, México, D.F., Mexico

    N. Torres-Torres

  4. I.P.N. Departamento de Graduados en Alimentos, Escuela Nacional de Ciencias Biológicas, Carpio y Plan de Ayala S/N Col. Sto. Tomás, 11340, México, D.F., Mexico

    R. Mora-Escobedo

Authors
  1. L. A. González-Espinosa de los Monteros
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  2. E. Ramón-Gallegos
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  3. N. Torres-Torres
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  4. R. Mora-Escobedo
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Correspondence to R. Mora-Escobedo.

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González-Espinosa de los Monteros, L.A., Ramón-Gallegos, E., Torres-Torres, N. et al. Effect of Germinated Soybean Protein Hydrolysates on Adipogenesis and Adipolysis in 3T3-L1 Cells. Plant Foods Hum Nutr 66, 355–362 (2011). https://doi.org/10.1007/s11130-011-0263-z

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