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Antioxidant and Antimelanogenic Behaviors of Paeonia suffruticosa

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Abstract

Antioxidant properties of eight Paeonia suffruticosa (Ps) extracts (Ps-1 to Ps-8) were evaluated. The respective half maximally effective concentration (EC50) values of Ps-1 ~ 8 were 10.0, 9.8, 63.6, >100, 3.8, 85.1, 6.9, and 0.7 μg/ml for 1,1-diphenyl-2-picrylhydrazyl radical (DPPH·) radical scavenging efficiency and 22.9, 11.4, 53.1, >100, 7.5, 97.6, 43.7, 4.2 μg/ml for 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS·+) radical scavenging capacity. The Ps-8 exhibited high free radical scavenging capacity, ion-chelating ability, reducing power, and inhibition of lipid peroxidation, which may have been attributable to its abundant phenolic and flavonoid content. In Hs68 and B16 cells treated with 100 μg/ml Ps-1, Ps-3, Ps-4 and Ps-6, expressions of toxic activities were lower than those in cells treated with arbutin and ascorbic acid. The antimelanogenesis properties were also tested in B16 cells. Extract Ps-1, and particularly extract Ps-6, considerably inhibited cellular tyrosinase and 3,4-dihydroxyphenylalanine (DOPA) oxidase activity and also reduced melanin content in B16 cells by down-expression of melanocortin-1 receptor (MC1R), microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase-related proteins-1 (TRP-1). The results suggest that P. suffruticosa extracts have antioxidant and antimelanogenesis activities with potential applications in cosmetic materials or food additives.

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Abbreviations

α-MSH:

α-melanocyte-stimulating hormone

DMSO:

Dimethyl sulfoxide

EC50 :

The half maximally effective concentration

MC1R:

Melanocortin-1 receptor

MITF:

Microphthalmia-associated transcription factor

Ps:

Paeonia suffruticosa

TBARS:

Thiobarbituric acid reactive substances

TEAC:

Trolox equivalent antioxidant capacity

TRP-1:

Tyrosinase-related proteins-1

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Authors and Affiliations

  1. Institute of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan

    Hsiou-Yu Ding

  2. Department of Chemical and Materials Engineering, National Yulin University of Science and Technology, Yulin, Taiwan

    Tzung-Han Chou

  3. Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan

    Guey-Horng Wang & Chia-Hua Liang

  4. Department of Parasitology, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

    Rong-Jyh Lin

  5. Department of Otolaryngology-Head and Neck Surgery, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan

    Leong-Perng Chan

Authors
  1. Hsiou-Yu Ding
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  2. Tzung-Han Chou
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  3. Rong-Jyh Lin
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  4. Leong-Perng Chan
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  5. Guey-Horng Wang
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  6. Chia-Hua Liang
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Correspondence to Chia-Hua Liang.

Electronic Supplementary Material

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Suppl. 1

Partition and fraction processes of P. suffruticosa. (DOC 30 kb)

Suppl. 2

HPLC chromatogram fingerprint pattern of Ps-6 and Ps-8 of P. suffruticosa. (a) Ps-6: peak 3, mudanpioside E; peak 10, mudanpioside B, and peak 15, suffrutioside E. (b) Ps-8: peak 1, tetragalloyl glucode; peak 2, pentagalloyl glucose; peak 3, galloylpaeoniflorin; peak 6, mudanpioside C; peak 7, benzyoylpaeoniflorin, and peak 9, mudanpioside B. (DOC 80 kb)

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Ding, HY., Chou, TH., Lin, RJ. et al. Antioxidant and Antimelanogenic Behaviors of Paeonia suffruticosa . Plant Foods Hum Nutr 66, 275–284 (2011). https://doi.org/10.1007/s11130-011-0235-3

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