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IgE-Mediated Cross-Reactivity among Leguminous Seed Proteins in Peanut Allergic Children

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Abstract

The immunological cross-reactivity among major protein- and oil-crops, including lupin, lentil, pea, peanut, kidney bean and soybean, has been studied by a combination of in vitro and in vivo experimental approaches: SDS-PAGE separations of legume protein extracts and immuno-blot revelations with 12 peanut-sensitive subjects’ sera, Immuno-CAP and Skin Prick tests on the same subjects. The immuno-blotting data showed a wide range of IgE-binding responses both displayed by one subject towards different plant extracts and among subjects. Differences were both quantitative and qualitative. The prevalent responses of most subjects’ sera were seen with peanut polypeptides, as expected, as well as with various polypeptides of the other legumes, the most recurrent of which were the basic subunits of the 11S globulins. The distribution of in vivo responses generally paralleled those obtained by in vitro approaches with strong responses elicited by peanut, lentil and pea protein extracts, especially by most sensitive subjects, thus providing a consistent overall set of results. In this work, the comparison of various approaches has allowed us to get an overall broad picture of the immunological cross-reactivities among proteins of widely used different seed species and to hypothesize the role of most conserved specific polypeptides.

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Authors and Affiliations

  1. Department of Pharmacological Sciences, Università degli Studi di Milano, via Balzaretti 9, 20133, Milan, Italy

    Cinzia Ballabio, Patrizia Restani & Maria Mottini

  2. Department of AgriFood Molecular Sciences, Università degli Studi di Milano, via Celoria 2, 20133, Milan, Italy

    Chiara Magni & Marcello Duranti

  3. Department of Child and Maternal Medicine, The Macedonio Melloni Hospital, Milan, Italy

    Alessandro Fiocchi

  4. Department of Animal Pathology, Hygiene and Veterinary Public Health-Section of Biochemistry, Università degli Studi di Milano, via Celoria 10, 20133, Milan, Italy

    Gabriella Tedeschi

Authors
  1. Cinzia Ballabio
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  2. Chiara Magni
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  3. Patrizia Restani
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  4. Maria Mottini
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  5. Alessandro Fiocchi
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  6. Gabriella Tedeschi
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  7. Marcello Duranti
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Correspondence to Marcello Duranti.

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Ballabio, C., Magni, C., Restani, P. et al. IgE-Mediated Cross-Reactivity among Leguminous Seed Proteins in Peanut Allergic Children. Plant Foods Hum Nutr 65, 396–402 (2010). https://doi.org/10.1007/s11130-010-0199-8

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