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High light acclimation of Chromera velia points to photoprotective NPQ

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Abstract

It has previously been shown that the long-term treatment of Arabidopsis thaliana with the chloroplast inhibitor lincomycin leads to photosynthetic membranes enriched in antennas, strongly reduced in photosystem II reaction centers (PSII) and with enhanced nonphotochemical quenching (NPQ) (Belgio et al. Biophys J 102:2761–2771, 2012). Here, a similar physiological response was found in the microalga Chromera velia grown under high light (HL). In comparison to cells acclimated to low light, HL cells displayed a severe re-organization of the photosynthetic membrane characterized by (1) a reduction of PSII but similar antenna content; (2) partial uncoupling of antennas from PSII; (3) enhanced NPQ. The decrease in the number of PSII represents a rather unusual acclimation response compared to other phototrophs, where a smaller PSII antenna size is more commonly found under high light. Despite the diminished PSII content, no net damage could be detected on the basis of the Photosynthesis versus irradiance curve and electron transport rates pointing at the excess capacity of PSII. We therefore concluded that the photoinhibition is minimized under high light by a lower PSII content and that cells are protected by NPQ in the antennas.

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Fig. 1

(modified from Belgio et al. 2012)

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Abbreviations

NPQ:

Nonphotochemical quenching

PSII:

Photosystem II reaction centers

HL:

High light

LL:

Low light

ΔpH:

Trans-thylakoid membrane proton gradient

F m :

Variable fluorescence

F m :

Maximum fluorescence

P max :

Maximal photosynthetic rate or photosynthetic capacity

TL:

Thermoluminescence

FRRF:

Fast repetition rate fluorescence

ETR:

Electron transport rates

σPSII:

Effective antenna size of PSII

I k :

Photosynthetic limiting light

I sat :

Light intensity where photosynthetic saturation starts

α-DM:

n-Dodecyl α-d-maltoside

DES:

De-epoxidation state

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Acknowledgements

The authors thank Dr. Martina Bečková and Ms. Lenka Moravcová for excellent technical help with electrophoresis and Dr. E. Lawrenz for useful discussions. This research project was supported by the Institutional project Algatech Plus (MSMT LO1416) from the Czech Ministry of Education, Youth and Sport. The work of E.B. and E.T. was further supported by The Czech Science Foundation GAČR (Grantová agentura České republiky): 16-10088S granted to R.K and 17-02363Y granted to E.B; GAJU 014/2016/P was granted to E.T.

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    1. Centre Algatech, Institute of Microbiology, Academy of Sciences of the Czech Republic, Opatovický mlýn, 379 81, Třeboň, Czech Republic

      Erica Belgio, Eliška Trsková, Eva Kotabová, Daniela Ewe, Ondřej Prášil & Radek Kaňa

    2. Faculty of Science, University of South Bohemia, Branišovská 31, 37005, Czech Budejovice, Czech Republic

      Eliška Trsková, Ondřej Prášil & Radek Kaňa

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    Erica Belgio and Eliška Trsková have contributed equally to this work.

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    Belgio, E., Trsková, E., Kotabová, E. et al. High light acclimation of Chromera velia points to photoprotective NPQ. Photosynth Res 135, 263–274 (2018). https://doi.org/10.1007/s11120-017-0385-8

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