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Comparison of oligomeric states and polypeptide compositions of fucoxanthin chlorophyll a/c-binding protein complexes among various diatom species

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Abstract

Fucoxanthin chlorophyll a/c-binding protein (FCP) is a unique light-harvesting apparatus in diatoms. Several biochemical characteristics of FCP oligomer and trimer from different diatom species have been reported previously. However, the integration of information about molecular organizations and polypeptides of FCP through a comparison among diatoms has not been published. In this study, we used two-dimensional clear-native/SDS-PAGE to compare the oligomeric states and polypeptide compositions of FCP complexes from four diatoms: Chaetoceros gracilis, Thalassiosira pseudonana, Cyclotella meneghiniana, and Phaeodactylum tricornutum. FCP oligomer was found in C. gracilis, T. pseudonana, and C. meneghiniana, but not in P. tricornutum. The oligomerization varied among the three diatoms, although a predominant subunit having similar molecular weight was recovered in each FCP oligomer. These results suggest that the predominant subunit is involved in the formation of high FCP oligomerization in each diatom. In contrast, FCP trimer was found in all the diatoms. The trimerizations were quite similar, whereas the polypeptide compositions were markedly different. On the basis of this information and that from mass spectrometric analyses, the gene products in each FCP complex were identified in T. pseudonana and P. tricornutum. Based on these results, we discuss the role of FCP oligomer and trimer from the four diatoms.

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Abbreviations

Chl:

Chlorophyll

Cg:

Chaetoceros gracilis

Cm:

Cyclotella meneghiniana

FCP:

Fucoxanthin chlorophyll a/c-binding protein

MS:

Mass spectrometric

Pt:

Phaeodactylum tricornutum

PS:

Photosystem

Tp:

Thalassiosira pseudonana

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Acknowledgments

This work was supported by Grant-in-Aids for Scientific Research from the Ministry of Education of Japan (No. 22370017, 24370025 to T.T.), a grant from JST PRESTO (T.T.), and a grant from the Australian Research Council’s Discovery Projects funding scheme (project number DP12101360) to T.T.

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Author notes

  1. Ryo Nagao

    Present address: Division of Material Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan

Authors and Affiliations

  1. Department of Integrated Sciences in Physics and Biology, College of Humanities and Sciences, Nihon University, 3-25-40 Sakurajosui, Setagaya-ku, Tokyo, 156-8550, Japan

    Ryo Nagao, Shuji Takahashi & Katsuyoshi Nakazato

  2. Biomolecular Characterization Team, RIKEN Advanced Science Institute, Hirosawa 2-1, Wako, Saitama, 351-0198, Japan

    Takehiro Suzuki & Naoshi Dohmae

  3. Department of Biology, Faculty of Science, Tokyo University of Science, Kagurazaka 1-3, Shinjuku-ku, Tokyo, 162-8601, Japan

    Tatsuya Tomo

  4. PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan

    Tatsuya Tomo

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  1. Ryo Nagao
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Correspondence to Tatsuya Tomo.

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Nagao, R., Takahashi, S., Suzuki, T. et al. Comparison of oligomeric states and polypeptide compositions of fucoxanthin chlorophyll a/c-binding protein complexes among various diatom species. Photosynth Res 117, 281–288 (2013). https://doi.org/10.1007/s11120-013-9903-5

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