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High-field EPR

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Abstract

Among the numerous spectroscopic techniques utilized in photosynthesis research, high-field/high-frequency EPR and its pulse extensions ESE, ENDOR, ESEEM, and PELDOR play an important role in the endeavor to understand, on the basis of structure and dynamics data, dominant factors that control specificity and efficiency of light-induced electron- and proton-transfer processes in primary photosynthesis. Short-lived transient intermediates of the photocycle can be characterized by high-field EPR techniques, and detailed structural information can be obtained even from disordered sample preparations. The chapter describes how multifrequency high-field EPR methodology, in conjunction with mutation strategies for site-specific isotope or spin labeling and with the support of modern quantum-chemical computation methods for data interpretation, is capable of providing new insights into the photosynthetic transfer processes. The information obtained is complementary to that of protein crystallography, solid-state NMR and laser spectroscopy.

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Abbreviations

EPR:

Electron paramagnetic resonance

mw:

Microwave

cw:

Continuous wave

TR:

Time resolved

rf:

Radio frequency

ENDOR:

Electron nuclear double resonance

PELDOR:

Pulsed electron electron double resonance

DEER:

Double electron electron resonance

DQC:

Double quantum coherence

ESEEM:

Electron spin echo envelope modulation

ESE:

Electron spin echo

PS:

Photosystem

RC:

Reaction center

ET:

Electron transfer

BChl:

Bacteriochlorophyll

BPhe:

Bacteriopheophytin

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Acknowledgments

It is a pleasure to thank all co-workers and cooperation partners for their contributions to the work described. We gratefully acknowledge essential encouragement and funding of interdisciplinary cooperative research by the Deutsche Forschungsgemeinschaft (SFB 337, SFB 498, III P5-MO 132/14-1, SPP 1051, MO 132/19-2), the Volkswagenstiftung (I/70 382, I/73 145) and the European Union (HCM, TMR, INTAS, COST P15).

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  1. Department of Physics, Free University Berlin, Arnimallee 14, 14195, Berlin, Germany

    Anton Savitsky & Klaus Möbius

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  1. Anton Savitsky
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  2. Klaus Möbius
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Correspondence to Klaus Möbius.

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Savitsky, A., Möbius, K. High-field EPR. Photosynth Res 102, 311–333 (2009). https://doi.org/10.1007/s11120-009-9432-4

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