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Salinity affects the photoacclimation of Chlamydomonas raudensis Ettl UWO241

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Abstract

Chlamydomonas raudensis Ettl UWO241, a natural variant of C. raudensis, is deficient in state transitions. Its habitat, the deepest layer of Lake Bonney in Antarctica, features low irradiance, low temperature, and high salinity. Although psychrophily and low-light acclimation of this green alga has been described, very little information is available on the effect of salinity. Here, we demonstrate that this psychrophile is halotolerant, not halophilic, and it shows energy redistribution between photosystem I and II based on energy spillover under low-salt conditions. Furthermore, we revealed that C. raudensis exhibits higher non-photochemical quenching in comparison with the mesophile Chlamydomonas reinhardtii, when grown with low-salt, which is due to the lower proton conductivity across the thylakoid membrane. Significance of the C. raudensis UWO241 traits found in the low salinity culture are implicated with their natural habitats, including the high salinity and extremely stable light environments.

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Abbreviations

F m :

Maximal yield of fluorescence.

Fm′:

Quenched maximal yield of fluorescence

LHC:

Light-harvesting complexes

NPQ:

Non-photochemical quenching

PQ:

Plastoquinone

PSI and PSII:

Photosystem I and II

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Acknowledgments

This work was supported in part by Grants-in-Aid for Scientific Research to J.M. from the Ministry of Education, Culture, Sports, Science and Technology (No. 18GS0318) and to N.P.A.H. from the Natural Sciences and Engineering Research Council of Canada.

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Authors and Affiliations

  1. Institute of Low Temperature Science, Hokkaido University, Sapporo, 060-0819, Japan

    Kenji Takizawa, Shinichiro Takahashi & Jun Minagawa

  2. Department of Biology and The Biotron, University of Western Ontario, London, ON, Canada, N6A 5B7

    Norman P. A. Hüner

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  1. Kenji Takizawa
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  2. Shinichiro Takahashi
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  3. Norman P. A. Hüner
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  4. Jun Minagawa
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Correspondence to Jun Minagawa.

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Takizawa, K., Takahashi, S., Hüner, N.P.A. et al. Salinity affects the photoacclimation of Chlamydomonas raudensis Ettl UWO241. Photosynth Res 99, 195–203 (2009). https://doi.org/10.1007/s11120-008-9397-8

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  • DOI: https://doi.org/10.1007/s11120-008-9397-8

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