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Multiple sites of retardation of electron transfer in Photosystem II after hydrolysis of phosphatidylglycerol

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Abstract

Phosphatidylglycerol (PG), containing the unique fatty acid Δ3, trans-16:1-hexadecenoic acid, is a minor but ubiquitous lipid component of thylakoid membranes of chloroplasts and cyanobacteria. We investigated its role in electron transfers and structural organization of Photosystem II (PSII) by treating Arabidopsis thaliana thylakoids with phospholipase A2 to decrease the PG content. Phospholipase A2 treatment of thylakoids (a) inhibited electron transfer from the primary quinone acceptor QA to the secondary quinone acceptor QB, (b) retarded electron transfer from the manganese cluster to the redox-active tyrosine Z, (c) decreased the extent of flash-induced oxidation of tyrosine Z and dark-stable tyrosine D in parallel, and (d) inhibited PSII reaction centres such that electron flow to silicomolybdate in continuous light was inhibited. In addition, phospholipase A2 treatment of thylakoids caused the partial dissociation of (a) PSII supercomplexes into PSII dimers that do not have the complete light-harvesting complex of PSII (LHCII); (b) PSII dimers into monomers; and (c) trimers of LHCII into monomers. Thus, removal of PG by phospholipase A2 brings about profound structural changes in PSII, leading to inhibition/retardation of electron transfer on the donor side, in the reaction centre, and on the acceptor side. Our results broaden the simple view of the predominant effect being on the QB-binding site.

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Abbreviations

BSA:

Bovine serum albumin

Chl:

Chlorophyll

CP43:

PSII core antenna-protein encoded by psbC

D1 and D2:

psbA and psbD gene product, respectively

DCMU:

3-(3,4-Dichlorophenyl)-1,1-dimethyl urea

EDTA:

Ethylenediamine tetra-acetic acid

EPR:

Electron paramagnetic resonance

PG:

Phosphatidylglycerol

PLA2 :

Phospholipase A2

PpBQ:

Phenyl-p-benzoquinone

PSI and PSII:

Photosystem I and II, respectively

P680 and P700 :

Photoactive Chl of the reaction centre of PSII and PSI, respectively

PQ:

Plastoquinone

QA and QB :

Primary and secondary plastoquinone electron acceptor of PSII

SQDG:

Sulfoquinovosyldiacylglycerol

YZ :

Redox-active tyrosine of D1 protein.

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Acknowledgments

An ANU/RSBS PhD Scholarship supported E-HK. We thank Dr Ron Pace and Dr Paul Smith for constructive comments on the manuscript.

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Author notes

  1. Reza Razeghifard

    Present address: Department of Anesthesiology, University of Michigan, 2215 Fuller Road, Ann Arbor, MI, 48105, USA

Authors and Affiliations

  1. Photobioenergetics Group, Research School of Biological Sciences, Australian National University, GPO Box 475, Canberra, ACT, 2601, Australia

    Eun-Ha Kim, Reza Razeghifard, Jan M. Anderson & Wah Soon Chow

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  1. Eun-Ha Kim
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Correspondence to Jan M. Anderson or Wah Soon Chow.

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Kim, EH., Razeghifard, R., Anderson, J. et al. Multiple sites of retardation of electron transfer in Photosystem II after hydrolysis of phosphatidylglycerol. Photosynth Res 93, 149–158 (2007). https://doi.org/10.1007/s11120-006-9126-0

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