Abstract
Three Chl–protein complexes were isolated from thylakoid membranes of Bryopsis maxima and Ulva pertusa, marine green algae that inhabit the intertidal zone of the Pacific Ocean off the eastern coast of Japan by dodecyl-β-d-maltoside polyacrylamide gel electrophoresis. The slowest-moving fractions showed low Chl a/b and Chl/P-700 ratios, indicating that this fraction corresponds to complexes in PS I, which is large in both algae. The intermediate and fastest-moving fractions showed the traits of PS II complexes, with some associated Chl a/b–protein complexes and LHC II, respectively. The spectral properties of the separated Chl–proteins were also determined. The absorption spectra showed a shallow shoulder at 540 nm derived from siphonaxanthin in Bryopsis maxima, but not in Ulva pertusa. The 77 K emission spectra showed a single peak in Bryopsis maxima and two peaks in Ulva pertusa. Besides the excitation spectra indicated that the excitation energy transfer to the PS I complexes differed quite a lot higher plants. This suggested that the mechanisms of energy transfer in both of these algae differ from those of higher plants. Considering the light environment of this coastal area, the large size of the antennae of PS I complexes implies that the antennae are arranged so as to balance light absorption between the two photosystems. In addition, we discuss the relationships among the photosystem stoichiometry, the energy transfer, and the distribution between the two photosystems.
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Abbreviations
- Chl:
-
Chlorophyll
- LHC I and LHC II:
-
Light-harvesting chlorophyll a/b–protein complexes associated with PS I and PS II, respectively
- P-700:
-
Photochemical reaction center of photosystem I
- PS:
-
Photosystem
- SDS-PAGE:
-
SDS-polyacrylamide gel electrophoresis
- β-DM:
-
Dodecyl-β-d-maltoside
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Acknowledgements
We dedicate this paper to the mentor Professor Yasumaro Kamimura who solved so many conundrums with such elegant experiments and insights. We also thank to Dr. Emiko Maruta and our laboratory collaborators for excellent technical assistant and continuous encouragement throughout this work.
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Yamazaki, Jy., Kozu, A. & Fukunaga, Y. Characterization of Chlorophyll–protein Complexes Isolated from Two Marine Green Algae, Bryopsis maxima and Ulva pertusa, Growing in the Intertidal Zone. Photosynth Res 89, 19–25 (2006). https://doi.org/10.1007/s11120-006-9064-x
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DOI: https://doi.org/10.1007/s11120-006-9064-x