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Expression of foreign type I ribulose-1,5-bisphosphate carboxylase/ oxygenase (EC 4.1.1.39) stimulates photosynthesis in cyanobacterium Synechococcus PCC7942 cells

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Abstract

A reporter gene assay revealed that promoters derived from Synechococcus PCC7942 (S.7942) psbAI and Synechocystis PCC6803 (S.6803) psbAII were suitable for the expression of foreign ribulose-bisphosphate carboxylase (RuBisCO; EC 4.1.1.39) in S.7942 cells. Transformational vectors with a promoter and a foreign RuBisCO gene, cvrbc originated from Allochromatium vinosum, were constructed on a binary vector, pUC303, and introduced to S.7942 cells. When the cvrbc was expressed with the S.7942 psbAI promoter, the total RuBisCO activity increased 2.5- to 4-fold than that of the wild type cell. The S.6803 psbAII promoter increased the activity of the transformant 1.5–2 times of that of wild type cell. There was a significant increase in the rate of photosynthesis depending on the increase of RuBisCO activity. The maximum rate of photosynthesis of the transformant cell was 1.63 times higher than that of the wild type under the illumination of 400 μmol m−2 s−1, at 20 mM bicarbonate and at 30 °C. Although the photosynthesis of the higher plant is limited by the ability of photosystems under high irradiance and the high CO2 concentration, that of the S.7942 cell is limited by the RuBisCO activity, even at high CO2 concentrations and under high irradiance.

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Abbreviations

RuBisCO:

ribulose−1,5-bisphosphate carboxylase/oxygenase

RPP cycle :

reductive pentose phosphate cycle

S.7942 :

Synechococcus PCC7942

S.6803 :

Synechocystis PCC6803

Ci:

inorganic carbon

References

  • Alam J, Cook JL, (1974) An improved spectrophotometric assay for ribulosebisphosphate carboxylase Biochim Biophys Acta 358: 226–229

    Google Scholar 

  • Andrews TJ, Abel KM, (1981) Kinetics and subunit interactions of ribulose bisphosphate carboxylase-oxygenase from the cyanobacterium, Synechococcus sp J Biol Chem 256: 8445–8451

    PubMed  CAS  Google Scholar 

  • Badger MR, (1980) Kinetic properties of ribulose 1,5-bisphosphate carboxylase/oxygenase from Anabaena variabilis Arch Biochem Biophys 201: 247–254

    Article  PubMed  CAS  Google Scholar 

  • Badger MR, Andrews TJ, Whitney SM, Ludwig M, Yellowlees DC, Leggat W, Price GD, (1998) The diversity and coevolution of RuBisCO, plastids, pyrenoids, and chloroplast-based CO2-concentrating mechanism in algae Can J Bot 76: 1052–1071

    Article  CAS  Google Scholar 

  • Badger MR, Price GD, (2003) CO2 concentrating mechanisms in cyanobacteria: molecular components, their diversity and evolution J Exp Bot 54: 609–622

    Article  PubMed  CAS  Google Scholar 

  • Badger MR, Sharkey TD, von Caemmerer S, (1984) The relationship between steady-state gas exchange of bean leaves and the levels of carbon-reduction-cycle intermediates Planta 160: 305–313

    Article  CAS  Google Scholar 

  • Bjorkman O, (1981) Responses to different quantum flux densities In: Lange OL, Nobel PS, Osmond CB, Ziegler H, (ed), Physiological Plant Ecology I Springer Verlag Berlin. pp 57–107

    Google Scholar 

  • Daniel H, Torres-Ruiz JA, McFadden BA, (1989) Amplified expression of ribulose bisphosphate carboxylase/oxygenase in pBR 322-transformants of Anacystis nidulans Arch Microbiol 151: 59–64

    Article  Google Scholar 

  • Farquhar GD, von Caemmerer S, (1982) Modeling of photosynthetic response to environmental conditions In: Lange OL, Nobel PS, Osmond CB, Ziegler H, (ed), Physiological Plant Ecology XII Springer Verlag Berlin pp 549–587

    Google Scholar 

  • Furbank RT, Chitty JA, von Caemmerer S, Jenkins CLD, (1966) Antisense RNA inhibition of RbcS gene expression reduces RuBisCO level and photosynthesis in the C4 plant Flaveria bidentis Plant Physiol 111: 725–734

    Google Scholar 

  • Hudson GS, Evans JR, von Caemmerer S, Arvidsson YBC, Andrews TJ, (1992) Reduction of ribulose 1,5-bisphosphate carboxylase/oxygenase content by antisense RNA reduces photosynthesis in transgenic tobacco plants Plant Physiol 98: 294–302

    PubMed  CAS  Google Scholar 

  • Laemmli UK, (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227: 680–685

    Article  PubMed  CAS  Google Scholar 

  • Laisk A, Oya VM, (1974) Photosynthesis of leaves subjected to brief pulses of CO2 Sov J Plant Physiol 21: 928–935

    Google Scholar 

  • Lilley RM, Walker DA, (1974) An improved spectrophotometric assay for ribulosebisphosphate carboxylase Biochim Biophys Acta 358: 226–229

    PubMed  CAS  Google Scholar 

  • Mackinney G, (1941) Absorption of light by chlorophyll solutions J Biol Chem 140: 315–322

    CAS  Google Scholar 

  • Makino A, Mae T, Ohira K, (1983) Photosynthesis and ribulose 1,5-bisphosphate carboxylase in rice leaves Plant Physiol 73: 1002–1007

    PubMed  CAS  Google Scholar 

  • Maniatis T, Fritsch EF and Sambrook J (ed) (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Springer Harbor, NY

  • Mott KA, Jensen RC, O’Leary JW, Berry JA, (1984) Photosynthesis and ribulose 1,5-bisphosphate concentrations in intact leaves of Xanthium strumarium L Plant Physiol 76: 968–971

    PubMed  CAS  Google Scholar 

  • Price GD, Badger MR, (1989) Ethoxyzolamide inhibition of CO2 uptake in the cyanobacterium Synechococcus PCC7942 without apparent inhibition of internal carbonic anhydrase activity Plant Physiol 89: 37–43

    PubMed  CAS  Google Scholar 

  • Price GD, Coleman JR, Badger MR, (1992) Association of carbonic anhydrase activity with carboxysomes isolated from the cyanobacterium Synechococcus PCC7942 Plant Physiol 100: 784–793

    PubMed  CAS  Google Scholar 

  • Rippka R, Waterbury JB, Stanier RY, (1981) Isolation and purification of cyanobacteria: some general principles In: Starr MP, Stolp H, Truper HG, Balows A, Schlegel HG, (ed), The Prokaryotes Springer Verlag Berlin pp 212–220

    Google Scholar 

  • Rodermel SR, Abbot MS, Bogorad L, (1988) Nuclear-organelle interactions: nuclear antisense gene inhibits ribulose bisphosphate carboxylase enzyme levels in transformed tobacco plants Cell 55: 673–681

    Article  PubMed  CAS  Google Scholar 

  • Satoh R, Himeno M, Wadano A, (1997) Carboxysomal diffusion resistance to ribulose 1,5-bisphosphate and 3-phosphoglycerate in the cyanobacterium Synechococcus PCC7942 Plant Cell Physiol 38: 769–775

    CAS  Google Scholar 

  • Shibata M, Katoh H, Sonoda M, Ohkawa H, Shimoyama M, Fukuzawa H, Kaplan A, Ogawa T, (2002b) Genes essential to sodium-dependent bicarbonate transport in cyanobacteria-function and phylogenetic analysis J Biol Chem 277: 18658–18664

    Article  CAS  Google Scholar 

  • Shibata M, Ohkawa H, Kaneko T, Fukuzawa H, Tabata S, Kaplan SA, Ogawa T, (2001) Distinct constitutive and low-CO2-induced CO2 uptake systems in cyanobacteria: genes involved and their phylogenetic relationship with homologous genes in other organisms Proc Natl Acad Sci 98: 11789–11794

    Article  PubMed  CAS  Google Scholar 

  • Shibata M, Ohkawa H, Katoh H, Shimoyama M, Ogawa T, (2002a) Two CO2-uptake systems: four systems for inorganic carbon acquisition in Synechocystis sp strain PCC6803 Funct Plant Biol 29: 123–129

    Article  CAS  Google Scholar 

  • Stevens SEJ, Poter RD, (1980) Transformation of Agmenellum quadruplicatum Proc Natl Acad. Sci 77: 6052–6056

    Article  PubMed  CAS  Google Scholar 

  • von Caemmerer S, Edmondson DL, (1986) Relationship between steady-state gas exchange, in vivo ribulose bisphosphate carboxylase activity and some carbon reduction cycle intermediates in Raphanus sativus Aust J Plant Physiol 13: 669–688

    Article  Google Scholar 

  • von Caemmerer S, Farquhar GD, (1981) Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves Planta 153: 376–387

    Article  Google Scholar 

  • Whitney SM, Baldet P, Hudson GS, Andrews TJ, (2001) Form I Rubiscos from non-green algae are expressed abundantly but not assembled in tobacco chloroplasts Plant J 26: 535–547

    Article  PubMed  CAS  Google Scholar 

  • Yokota A, Asama K, Kitaoka S, (1990) Evidence of reentrance of glycolate carbon into the photosynthetic carbon reduction cycle in phtotosynthesizing Euglena gracilis Z Plant Physiol 77: 735–739

    Article  Google Scholar 

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Authors and Affiliations

  1. Graduate Scholl of Life and Environmental Sciences, Osaka Prefecture University, Gakuen-cho 1-2, 599-8231, Osaka, Sakai, Japan

    T. Iwaki, K. Haranoh, N. Inoue, K. Kojima, R. Satoh, T. Nishino, S. Wada, S. Tsuyama & A. Wadano

  2. Graduate Scholl of Sciences, Osaka Prefecture University, Gakuen-cho 1-2, 599-8231, Osaka, Sakai, Japan

    H. Ihara

  3. School of Food and Nutritional Sciences, University of Shizuoka, Yada 52-1, 422-8526, Shizuoka city, Shizuoka , Japan

    H. Kobayashi

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  1. T. Iwaki
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  2. K. Haranoh
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  3. N. Inoue
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  4. K. Kojima
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  5. R. Satoh
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  6. T. Nishino
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  7. S. Wada
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  9. S. Tsuyama
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  10. H. Kobayashi
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  11. A. Wadano
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Correspondence to A. Wadano.

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Iwaki, T., Haranoh, K., Inoue, N. et al. Expression of foreign type I ribulose-1,5-bisphosphate carboxylase/ oxygenase (EC 4.1.1.39) stimulates photosynthesis in cyanobacterium Synechococcus PCC7942 cells. Photosynth Res 88, 287–297 (2006). https://doi.org/10.1007/s11120-006-9048-x

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