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Quantification of non-Q B -reducing centers in leaves using a far-red pre-illumination

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Abstract

An alternative approach to quantification of the contribution of non-Q B -reducing centers to Chl a fluorescence induction curve is proposed. The experimental protocol consists of a far-red pre-illumination followed by a strong red pulse to determine the fluorescence rise kinetics. The far-red pre-illumination induces an increase in the initial fluorescence level (F25 μs) that saturates at low light intensities indicating that no light intensity-dependent accumulation of Q −A occurs. Far-red light-dose response curves for the F25 μs-increase give no indication of superimposed period-4 oscillations. F25 μs-dark-adaptation kinetics following a far-red pre-pulse, reveal two components: a faster one with a half-time of a few seconds and a slower component with a half-time of around 100 s. The faster phase is due to the non-Q B -reducing centers that re-open by recombination between Q −A and the S-states on the donor side. The slower phase is due to the recombination between Q −B and the donor side in active PS II reaction centers. The pre-illumination-induced increase of the F25 μs-level represents about 4–5% of the variable fluorescence for pea leaves (∼2.5% equilibrium effect and 1.8–3.0% non-Q B -reducing centers). For the other plant species tested these values were very similar. The implications of these values will be discussed.

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Abbreviations

Chl:

chlorophyll

DCMU:

3-(3,4-dichlorophenyl)-1,1′-dimethylurea

F25 μ s:

initial fluorescence, for dark adapted leaves equal to Fo

Fpl:

intermediate fluorescence induction level when measured at low light intensity

Fv:

variable fluorescence here used as maximum variable fluorescence

LED:

light emitting diode

OJIP curve:

fluorescence induction transient defined by the names of its intermediate steps, O-level is fluorescence-level at 25 μs, J-level is fluorescence-level at ∼2 ms, I-level is fluorescence level at ∼30 ms and P-level is F m , the maximum fluorescence

QA and QB:

primary and secondary quinone electron acceptors of Photosystem II

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  1. Bioenergetics Laboratory, University of Geneva, Chemin des Embrouchis 10, 1254, Jussy, Geneva, Switzerland

    Gert Schansker & Reto J. Strasser

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  1. Gert Schansker
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  2. Reto J. Strasser
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Correspondence to Gert Schansker.

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Schansker, G., Strasser, R.J. Quantification of non-Q B -reducing centers in leaves using a far-red pre-illumination. Photosynth Res 84, 145–151 (2005). https://doi.org/10.1007/s11120-004-7156-z

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