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Evidence for localisation of accumulated chlorophyll cation on the D1-accessory chlorophyll in the reaction centre of Photosystem II

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Abstract

Absorption and circular dichroism spectra of Photosystem II (PS II) reaction centres (RC) were studied and compared with spectra calculated on the basis of point-dipole approximation. Chlorophyll cation was accumulated during a light treatment of PS II RC in the presence of artificial electron acceptor silicomolybdate. Light-induced difference spectra and their calculated counterparts revealed the location of accumulated cation at the accessory chlorophyll of the D1 protein subunit.

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Abbreviations

CD:

circular dichroism

Chl:

chlorophyll

D1:

psbA gene product

D2:

psbD gene product

DM:

n-dodecyl-β-D-maltoside

FWHM:

full-width at half maximum

MES:

morpholinethanesulfonic acid

Pheo:

pheophytin

PS II:

Photosystem II

RC:

reaction centre

SiMo:

silicomolibdic acid

References

  • J Barber DJ Chapman A Telfer (1987) ArticleTitleCharacterisation of a PS II reaction centre isolated from the chloroplasts of Pisum sativum FEBS Lett 220 67–73 Occurrence Handle10.1016/0014-5793(87)80877-3

    Article  Google Scholar 

  • LMC. Barter JR. Durrant DR. Klug (2003) ArticleTitleA quantitative structure-function relationship for the Photosystern II reaction center: supermolecular behavior in natural photosynthesis Proc Natl Acad Sci USA 100 946–951 Occurrence Handle12538865

    PubMed  Google Scholar 

  • FTH. den Hartog F. Vacha AJ. Lock J. Barber JP. Dekker S. Volker (1998) ArticleTitleComparison of the excited state dynamics of five- and six-chlorophyll Photosystem II reaction center complexes J Phys Chem 102 9174–9180

    Google Scholar 

  • BA Diner F Rappaport (2002) ArticleTitleStructure, dynamiscs and energetics of the primary photochemistry of Photosystem II of oxygenic photosynthesis Annu Rev Plant Biol 53 551–580 Occurrence Handle12221988

    PubMed  Google Scholar 

  • BA Diner E Schlodder JP Nixon WJ Coleman F Rappaport J Lavergne WFJ Vermaas DA Chisholm (2001) ArticleTitleSite-directed mutations at D1-His198 and D2-His 97 of Photosystem II in synechocystis PCC 6803: sites of primary charge separation and cation and triplet stabilization Biochemistry 40 9265–9281 Occurrence Handle11478894

    PubMed  Google Scholar 

  • JR Durrant DR Klug SLS Kwa R Grondelle Particlevan G Porter JP Dekker (1995) ArticleTitleA multimer model for P680, the primary electron donor of Photosystem II Proc Natl Acad Sci USA 92 4798–4802 Occurrence Handle11607546

    PubMed  Google Scholar 

  • C Eijckelhoff JP Dekker (1995) ArticleTitleDetermination of the pigment stochiometry of the photochemical reaction center of Photosystem II Biochim Biophys Acta 1231 21–28

    Google Scholar 

  • C Eijckelhoff F Vacha R Grondelle Particlevan JP Dekker J Barber  (1997) ArticleTitleSpectroscopic characterization of a 5 Chl a Photosystem II reaction center complex Biochim Biophys Acta 1318 266–274

    Google Scholar 

  • KN Ferreira TM Iverson K Maghlaoui J Barber S Iwata (2004) ArticleTitleArchitecture of the photosynthetic oxygen-evolving center Science 303 1831–1838 Occurrence Handle14764885

    PubMed  Google Scholar 

  • R Jankowiak JM Hayes GJ Small (2002) ArticleTitleAn excitonic pentamer model for the core Q(y) states of the isolated Photosystem II reaction center J Phys Cem B 106 8803–8814 Occurrence Handle10.1021/jp020050l

    Article  Google Scholar 

  • N Kamiya J-R Shen (2003) ArticleTitleCrystal structure of oxygen-evolving Photosystem II from Thermosynechococcus vulcanus at 3.7-angstrom resolution Proc Natl Acad Sci USA 100 98–103 Occurrence Handle12518057

    PubMed  Google Scholar 

  • L Konermann AR Holzwarth (1996) ArticleTitleAnalysis of the absorption spectrum of Photosystem II reaction centers: temperature dependence, pigment assignment, and inhomogeneous broadening Biochemistry 35 829–842 Occurrence Handle8547263

    PubMed  Google Scholar 

  • N Nanba K Satoh (1987) ArticleTitleIsolation of a Photosystem II reaction center containing D-1 and D-2 polypeptides and␣cytochrome b-559 Proc Natl Acad Sci USA 84 109–112

    Google Scholar 

  • T Noguchi T Tomo Y Inoue (1998) ArticleTitleFourier transform infrared study of the cation radical of P680 in the Photosystem II reaction center: evidence for charge delocalization on the chlorophyll dimer Biochemistry 37 13614–13625 Occurrence Handle9753448

    PubMed  Google Scholar 

  • RM Pearlstein (1991) Theoretical interpretation of antenna spectra H Scheer (Eds) Chlorophylls. CRC Press Bocca Raton FL 1047–1078

    Google Scholar 

  • VI Prokhorenko AR Holzwarth (2000) ArticleTitlePrimary processes and structure of the Photosystem II reaction center: a photon echo study J Phys Cem B 104 11563–11578 Occurrence Handle10.1021/jp002323n

    Article  Google Scholar 

  • J Psencik YZ Ma JB Arellano J Hala T Gillbro (2003) ArticleTitleExcitation energy transfer dynamics and excited-state structure in chlorosomes of Chlorobium phaeobacteroides Biophys J 84 1161–1179 Occurrence Handle12547796

    PubMed  Google Scholar 

  • B Svensson C Etchebest P Tuffery P Kan Particlevan J Smith S Styring (1996) ArticleTitleA model for the Photosystem II reaction center core including the structure of the primary donor P-680 Biochemistry 35 14486–14502 Occurrence Handle8931545

    PubMed  Google Scholar 

  • F Vacha DM Joseph JR Durrant A Telfer DR Klug G Porter J Barber (1995) ArticleTitlePhotochemistry and spectroscopy of a five-chlorophyll reaction center of Photosystem II isolated by using a Cu affinity column Proc Natl Acad Sci 92 2929–2933 Occurrence Handle11607522

    PubMed  Google Scholar 

  • F Vacha M Durchan P. Siffel (2002) ArticleTitleExcitonic interactions in the reaction centre of Photosystem II studied by using␣circular dichroism Biochim Biophys Acta 1554 147–152 Occurrence Handle12160987

    PubMed  Google Scholar 

  • ME Brederode Particlevan R Grondelle Particlevan (1999) ArticleTitleNew and unexpected routes for ultrafast electron transfer in photosynthetic reaction centers FEBS Lett 455 1–7 Occurrence Handle10428460

    PubMed  Google Scholar 

  • J Xiong S Subramaniam Govindjee (1998) ArticleTitleA knowledge-based three dimensional model of the Photosystem II reaction center of Chlamydomonas reinhardtii Photosynth Res 56 229–254 Occurrence Handle10.1023/A:1006061918025

    Article  Google Scholar 

  • D Zheleva B Hankamer J Barber (1996) ArticleTitleHeterogeneity and pigment composition of isolated Photosystem II reaction centers Biochemistry 35 15074–15079 Occurrence Handle8942674

    PubMed  Google Scholar 

  • A Zouni HT Witt J Kern P Fromme N Krauss W Saenger P Orth (2001) ArticleTitleCrystal structure of Photosystem II from Synechococcus elongatus at 3.8 angstrom resolution Nature 409 739–743 Occurrence Handle11217865

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Physical Biology, University of South Bohemia, Zamek 136, 373 33, Nove Hrady, Czech Republic

    Frantisek Vacha, Jakub Psencik, Michal Kuty, Milan Durchan & Pavel Siffel

  2. Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic

    Frantisek Vacha, Milan Durchan & Pavel Siffel

  3. Faculty of Mathematics and Physics, Charles University, Ke Karlovu 3, 121 16, Prague, Czech Republic

    Jakub Psencik

  4. Institute of Landscape Ecology, Academy of Sciences of the Czech Republic, Zamek 136, 373 33, Nove Hrady, Czech Republic

    Michal Kuty

Authors
  1. Frantisek Vacha
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  2. Jakub Psencik
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  3. Michal Kuty
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  4. Milan Durchan
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  5. Pavel Siffel
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Correspondence to Frantisek Vacha.

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Vacha, F., Psencik, J., Kuty, M. et al. Evidence for localisation of accumulated chlorophyll cation on the D1-accessory chlorophyll in the reaction centre of Photosystem II. Photosynth Res 84, 297–302 (2005). https://doi.org/10.1007/s11120-004-6817-2

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  • DOI: https://doi.org/10.1007/s11120-004-6817-2

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