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Expression Profiles of Pathogenesis-Related Gene, TaLr35PR1, as it Relate to Lr35-Mediated Adult Plant Leaf Rust Resistance

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Abstract

Plants have developed sophisticated mechanisms to combat pathogen infection. One of the acquired modes in response to pathogen attack is the production of the pathogenesis-related (PR) proteins. Our earlier studies reported that TaLr35PR1, a PR1 gene encoding a protein with conserved serine carboxypeptidase (SCP) domain, has been cloned from wheat near-isogenic line TcLr35. However, the involvement of TaLr35PR1 in wheat growth and Lr35-mediated adult resistance to Puccinia triticina remains unclear. Here, we showed that TaLr35PR1 was strongly induced by P. triticina in wheat plant containing Lr35 (TcLr35), in which the expression level of TaLr35PR1 significantly increased and reached the maximum at 12 hpi. The accumulations of TaLr35PR1 increased stably and showed significant peak challenged by P. triticina at different growth and development periods of TcLr35 wheat while it maintained similar level and changed little in mock inoculated. Western blotting was conducted to confirm that TaLr35PR1 protein was increasingly accumulated in the TcLr35 adult plants after P. triticina inoculation and maintained at a similar level from 120 to 168 h post-inoculation. Similar to the expression patterns of TaLr35PR1 at RNA levels, the accumulations of TaLr35PR1 protein were weak in the seedling stage and then increased to the peak and kept constant levels at the mature stage which is consistent with the expression feature of Lr35 gene as an adult plant resistance gene. All these findings suggest that TaLr35PR1 is involved in wheat growth and Lr35-mediated adult wheat defense response to leaf rust pathogen attack.

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Abbreviations

APR:

Adult plant resistance

PRs:

Pathogenesis-related proteins

SA:

Salicylic acid

ET:

Ethylene

SAR:

Systemic acquired resistance

SCP:

Serinecarboxy peptidase

IPTG:

Isopropyl-b-D-thiogalactopyranoside

GAPDH:

Glyceraldehyde 3-phosphate dehydrogenase gene

SPSS:

Statistical Package for the Social Sciences

HSP:

Heat shock proteins

WB:

Western blot

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Acknowledgments

This study was funded by the National Natural Science Foundation of China (No. 31501623) and Hebei Education Department Program (No. QN2015171).

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Authors and Affiliations

  1. College of Plant Protection, Agricultural University of Hebei, Baoding, China

    Xiaoying Li, Yanjun Zhang, Weihong Zhang, Jiarui Zhang, Haiyan Wang & Daqun Liu

  2. Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, Baoding, 071000, China

    Xiaoying Li, Yanjun Zhang, Weihong Zhang, Jiarui Zhang, Haiyan Wang & Daqun Liu

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  1. Xiaoying Li
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  2. Yanjun Zhang
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  3. Weihong Zhang
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  4. Jiarui Zhang
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  5. Haiyan Wang
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Correspondence to Haiyan Wang or Daqun Liu.

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Li, X., Zhang, Y., Zhang, W. et al. Expression Profiles of Pathogenesis-Related Gene, TaLr35PR1, as it Relate to Lr35-Mediated Adult Plant Leaf Rust Resistance. Plant Mol Biol Rep 34, 1127–1135 (2016). https://doi.org/10.1007/s11105-016-0992-0

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