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Investigating the Role of OsPDCD5, a Homolog of the Mammalian PDCD5, in Programmed Cell Death by Inducible Expression in Rice

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Abstract

OsPDCD5 is the rice (Oryza sativa L.) homolog of PDCD5, a gene involved in programmed cell death (PCD) regulation and highly conserved over time. PDCD5 overexpression in tumor cells enhances apoptosis triggered by growth factor or serum deprivation. In our previous research, constitutive OsPDCD5 overexpression induces early death in transgenic plants. In this study, an inducible OsPDCD5 expression strategy was employed to systematically study the role of OsPDCD5 in rice. With this system, OsPDCD5 was found to be capable of independently inducing cell death in three-leaf stage and older seedlings. These altered plants exhibited lesion mimic phenotype, abnormal leaf morphology, DNA fragmentation, hydrogen peroxide production, and mitochondrial distortion. In nature, RNA hybridization in situ has shown that OsPDCD5 expression was predominantly localized to the tapetal layer where PCD occurred. Transcript microarray analyses here revealed that many PCD-related genes were involved. These data, taken together, indicated that OsPDCD5-induced cell death is a kind of PCD. Nevertheless, OsPDCD5 failed to induce any visible morphological phenotypes in two-leaf stage and younger seedlings. Transcript microarray analyses and quantitative real-time polymerase chain reaction showed that the Bax inhibitor-1 (BI-1) mRNA concentration and the activity of an ubiquitin gene were specifically changed. Endogenous OsPDCD5 upregulation, induced by ectopic OsPDCD5 expression in three-leaf stage seedlings, was also absent in two-leaf stage seedlings, suggesting strongly that young seedlings could inhibit PCD at some level.

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Acknowledgments

This research was supported by grants from the Major State Basic Research Development Program of China (973 Program, No. 2007CB109002) and the Foundation of State Key Laboratory of Gene Engineering, Fudan University.

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  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai, 200433, People’s Republic of China

    Fan Sun, Weiwei Qi, Xiaoyin Qian, Qianjie Wang, Mengfei Yang, Xianxin Dong & Jinshui Yang

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  1. Fan Sun
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  2. Weiwei Qi
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Correspondence to Jinshui Yang.

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Sun, F., Qi, W., Qian, X. et al. Investigating the Role of OsPDCD5, a Homolog of the Mammalian PDCD5, in Programmed Cell Death by Inducible Expression in Rice. Plant Mol Biol Rep 30, 87–98 (2012). https://doi.org/10.1007/s11105-011-0307-4

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