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High-Oleic Peanut Oils Produced by HpRNA-Mediated Gene Silencing of Oleate Desaturase

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Abstract

The quality of peanut oil largely depends on the quantity of oleic (18:1) and linoleic acids (18:2). These two acids comprise more than 80% of the total fatty acids in peanuts. The oleate desaturase (FAD2) gene is important for maintaining high oleic acid content. A partial conservative sequence of the FAD2 gene from peanut was selected. The sense and antisense 260-bp fragments were amplified and subcloned into pFGC1008 binary expression vectors. A total of 21 transgenic plants were obtained via Agrobacterium-mediated transformation. The resulting down-regulation of the FAD2 gene resulted in a 70% increase in oleic acid content in the seeds of transformed plants compared with a 37.93% increase in untransformed plants. The results demonstrated that the target genes were likely suppressed by hpRNA interference, a pathway capable of achieving phenotypic changes. The silencing of FAD2 enabled the development of peanut oils having novel combinations of oleic acid content that can be used in high-value applications, making this approach a reliable technique for the genetic modification of seed quality and the potential for enhancement of other traits as well.

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Acknowledgement

Many thanks to National Natural Science Foundation of China for the financial assistance. We also thank Ph.D Zifu Yan for critical reading of the manuscript.

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Authors and Affiliations

  1. Department of Agronomy, Henan Agricultural University, 450002, Zhengzhou, China

    Dongmei Yin, Shizheng Deng, Kehui Zhan & Dangqun Cui

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  1. Dongmei Yin
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  2. Shizheng Deng
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  3. Kehui Zhan
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  4. Dangqun Cui
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Correspondence to Dangqun Cui.

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Yin, D., Deng, S., Zhan, K. et al. High-Oleic Peanut Oils Produced by HpRNA-Mediated Gene Silencing of Oleate Desaturase. Plant Mol Biol Rep 25, 154–163 (2007). https://doi.org/10.1007/s11105-007-0017-0

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