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Effect of different decay classes of Eucalyptus stump substrates on microbial resource limitation and carbon-use efficiency

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Abstract

Aims

The effect of Eucalyptus stump substrate decomposition on microbial resource limitation driving factors are unclear.

Methods

Eucalyptus stump substrate samples from five decay classes with contrasting qualities were decomposed in Eucalyptus undergrowth soil for three months in a laboratory microcosm experiment. We determined the substrate quality, microbial biomass, microbial community structure, and extracellular enzyme activities for the decay classes of stump substrates.

Results

The stump carbon:nitrogen (C:N), carbon:phosphorus (C:P), and nitrogen:phosphorus (N:P) ratios and dissolved organic carbon:total dissolved nitrogen (DOC:TDN), dissolved organic carbon:available phosphorus (DOC:AP), and total dissolved nitrogen:available phosphorus (TDN:AP) ratios increased and then decreased as the decay class increased, whereas the C:N and C:P imbalances between the microorganisms inhabiting stumps and their resources increased. The activities of N- and P-acquiring enzymes improved as the decay class increased, and such changes improved the microbial N and P limitation of stumps and reduced the microbial C use efficiency. In advanced decay classes, the microbial community structure shifted toward a higher ratio of fungi to bacteria and a lower ratio of gram-positive bacteria to gram-negative bacteria. Redundancy analysis showed that P, DOC, and DOC:AP were all closely related to the enzyme activities, whereas P and N were the critical factors alter microbial composition community. In addition, ecoenzymatic stoichiometry and the C:N:P imbalance played key roles on regulating microbial C use efficiency.

Conclusions

The decomposition of Eucalyptus stump substrates modified microbial communities and enzymatic stoichiometry, leading to microbial growth from P limitation to N limitation.

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Funding

This research was provided by the “Dynamic changes in microbial communities during decomposition of Eucalyptus stumps based on ecological stoichiometry” (2017GXNSFAA198007) from the Guangxi Natural Science Foundation, “Characteristics of microbial communities during decomposition of underground coarse roots of Eucalyptus stumps” (32160359) from the National Natural Science Foundation of China.

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  1. Forestry College, Guangxi University, Nanning, 530004, Guangxi, China

    Xiangsheng Deng, Fei Cheng, Mingman Li, Peng He, Lu Shen & Haiyu Liu

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  1. Xiangsheng Deng
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Deng, X., Cheng, F., Li, M. et al. Effect of different decay classes of Eucalyptus stump substrates on microbial resource limitation and carbon-use efficiency. Plant Soil 478, 651–669 (2022). https://doi.org/10.1007/s11104-022-05499-x

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