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Proteomics combined with BSMV-VIGS methods identified some N deficiency-responsive protein species and ABA role in wheat seedling

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Abstract

Aims

Crops often encounter a soil deficiency of nitrogen (N), the most important macronutrient for plants; however, the molecular mechanism of plant responses to N deficiency remains unclear. In this study, proteome-level changes that occur in bread wheat seedlings suffering from N deficiency were investigated to identify some N deficiency-responsive protein species in bread wheat.

Methods

We utilized isobaric tagging for relative and absolute quantification (iTRAQ) to measure changes in the proteome patterns of N-deficient wheat seedlings and validated the role of abscisic acid (ABA) using the barley stripe mosaic virus-induced gene-silencing (BSMV-VIGS) method.

Results

A total of 1515 N deficiency–responsive protein species were successfully identified in both root and leaf tissues of wheat seedlings suffering from 8-d N deficiency. Of these, abundance of wheat zeaxanthin epoxidase (TaZEP), a key ABA synthesis-related enzyme, was significantly upregulated, and the endogenous ABA contents also markedly increased. After TaZEP gene was further silenced using BSMV-VIGS method, BSMV-VIGS-TaZEP infected wheat seedlings showed enhanced sensitivity to N deficiency, suggesting silencing of TaZEP gene decreased the tolerance to N deficiency remarkably.

Conclusion

Our results identified some N deficiency-responsive protein species and revealed the role of ABA in wheat responses to N deficiency.

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Abbreviations

ABA:

abscisic acid

BSMV-VIGS:

barley stripe mosaic virus-virus induced gene-silencing

CTK:

cytokinin

DW:

dry weight

ETH:

ethylene

FW:

fresh weight

IAA:

auxin

iTRAQ:

isobaric tagging for relative and absolute quantification

JA:

jasmonic acid

MW:

molecular weights

pI:

isoelectric points

qRT-PCR:

quantitative real-time PCR

SA:

salicylic acid

ZEP:

zeaxanthin epoxidase

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Acknowledgements

This study was financially supported by the Science and Technology Innovation Program for Increase in Yield and Efficiency of Food Crop (2016YFD0300105) and the National Key Technology Support Program (2015BAD26B01).

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Authors and Affiliations

  1. Collaborative Innovation Center of Henan Food Crops, Henan Agricultural University, Zhengzhou, 450002, People’s Republic of China

    Guozhang Kang, Yufang Wu, Pengfei Wang, Yonghua Wang & Tiancai Guo

  2. National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou, 450002, People’s Republic of China

    Gezi Li, Pengfei Wang, Qiaoxia Han, Yonghua Wang & Yingxin Xie

  3. The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, 450002, People’s Republic of China

    Pengfei Wang, Wei Feng, Dongyun Ma & Chenyang Wang

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  1. Guozhang Kang
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  2. Yufang Wu
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  3. Gezi Li
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  4. Pengfei Wang
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  5. Qiaoxia Han
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  10. Chenyang Wang
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Correspondence to Tiancai Guo.

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Kang, G., Wu, Y., Li, G. et al. Proteomics combined with BSMV-VIGS methods identified some N deficiency-responsive protein species and ABA role in wheat seedling. Plant Soil 444, 177–191 (2019). https://doi.org/10.1007/s11104-019-04260-1

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