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A half-type ABC transporter FeSTAR1 regulates Al resistance possibly via UDP-glucose-based hemicellulose metabolism and Al binding

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Abstract

Aims

Buckwheat (Fagopyrum esculentum) is highly tolerant to Al stress, but the molecular mechanisms remain largely unknown. This study aims to investigate a half-type ABC transporter gene (FeSTAR1) with respect to Al tolerance.

Methods

The expression of FeSTAR1 was examined and complementation test in atstar1 mutant was conducted. Furthermore, Al distribution and cell wall polysaccharides content were analyzed.

Results

FeSTAR1 is an ABC transporter protein with nucleotide binding domain, but lack of transmembrane domain. Consistently, FeSTAR1 is a soluble protein, localizing to both cytoplasm and nucleus. Al rapidly and specifically induced FeSTAR1 expression. Heterologous expression of FeSTAR1 in atstar1 rescued its Al tolerance, and exogenous applied UDP-glucose could alleviate Al sensitivity of atstar1 mutant, suggesting the connection between FeSTAR1 and UDP-glucose in terms of Al tolerance. Furthermore, FeSTAR1 complemented lines accumulated less Al in root cell wall than atstar1 mutant. Further cell wall fraction analysis showed that Al was largely confined to cell wall hemicellulose1, at which Al content was significantly lower in complemented lines. Consistent with Al distribution in different cell wall polysaccharides, complemented lines had lower hemicellulose1 content.

Conclusion

Our results indicate that FeSTAR1 is involved in Al resistance via possibly cell wall matrix polysaccharides metabolism in buckwheat.

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Acknowledgements

This work was supported financially by the Natural Science Foundation of China (31572193) and The Chang Jiang Scholars Program (JLY). We are grateful to Prof. Chaofeng Huang for providing us the Arabidopsis mutant atstar1 seeds.

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Authors and Affiliations

  1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China

    Jia Meng Xu, Jian Feng Jin, Jiang Xue Wan & Jian Li Yang

  2. State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A& F University, Lin’an, Zhejiang, 311300, China

    He Qiang Lou

  3. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China

    Wei Wei Chen

  4. State Key Laboratory of Conservation and Utilization of Bio-resources in Yunnan, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, National & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming, 650201, China

    Wei Fan

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  1. Jia Meng Xu
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  3. Jian Feng Jin
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  4. Wei Wei Chen
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Correspondence to Jian Li Yang.

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Responsible Editor: Juan Barcelo.

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Xu, J.M., Lou, H.Q., Jin, J.F. et al. A half-type ABC transporter FeSTAR1 regulates Al resistance possibly via UDP-glucose-based hemicellulose metabolism and Al binding. Plant Soil 432, 303–314 (2018). https://doi.org/10.1007/s11104-018-3805-4

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  • DOI: https://doi.org/10.1007/s11104-018-3805-4

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