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Differences in root-associated bacterial communities among fine root branching orders of poplar (Populus × euramericana (Dode) Guinier.)

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Abstract

Background and Aims

Root branching leads to morphological and functional heterogeneity of fine roots. However, the structure of soil microbiota associated with root branching orders has never been investigated. Deep insights into rhizosphere microbial community could provide a better understanding of the plant-microbe relationship.

Methods

Fine roots of poplar (Populus × euramericana (Dode) Guinier.) were sampled and sorted into three groups according to their branching orders. Scanning electron microscopy (SEM) was used to observe the surface features of different orders of fine roots. Illumina MiSeq was employed to analyze the bacterial community structure of soil compartments from different root orders (i.e., R1, R2, and R3) and bulk soil compartment (NR).

Results

SEM showed that the first-order root with smaller diameter had dense coverage of vigorous root hairs, whereas higher order roots with larger diameter had sloughed-off cortical tissues on the rhizoplane. The diversity of bacterial communities was higher in the R1 and R2 compartment than in R3 or NR. There were 80 genera with a relative abundance above 0.05% in soils. Ternary plot revealed that bacterial genera were significantly enriched in R1 than in R2 or R3. Redundant analysis (RDA) showed that 12 dominant bacterial genera with a relative abundance over 1% were significantly correlated with P and NH4 +-N content of soils.

Conclusions

Root orders could influence the inhabitation of bacterial communities. The core groups of bacterial communities inhabiting the rhizosphere were correlated with soil nutrients. The root order-dependent interactions of plant-microbe provided a new model about the association between roots and soils.

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Acknowledgments

This work was funded by the National Basic Research Program of China (2012CB416904) and the National Natural Science Foundation of China (31570618). Additionally, this study was supported by the funds of the Shandong Double Tops Program (Grant No. SYL2017XTTD03). We thank the anonymous reviewers for the helpful comments.

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Author notes

  1. Qitong Wang and Nian Wang contributed equally to this work.

Authors and Affiliations

  1. Taishan Forest Ecosystem Research Station of State Forestry Administration, College of Forestry, Shandong Agricultural University, 61 Daizong Street, Tai’an, 271018, People’s Republic of China

    Qitong Wang, Nian Wang & Yanping Wang

  2. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110164, People’s Republic of China

    Qingkui Wang

  3. Institute of Mountain Hazards and Environments, Chinese Academy of Sciences, Chengdu, 610041, People’s Republic of China

    Baoli Duan

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  1. Qitong Wang
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  2. Nian Wang
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  3. Yanping Wang
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  4. Qingkui Wang
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Correspondence to Yanping Wang.

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Responsible Editor: Stéphane Compant

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Wang, Q., Wang, N., Wang, Y. et al. Differences in root-associated bacterial communities among fine root branching orders of poplar (Populus × euramericana (Dode) Guinier.). Plant Soil 421, 123–135 (2017). https://doi.org/10.1007/s11104-017-3449-9

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