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Genome-scale mRNA transcriptomic insights into the responses of oilseed rape (Brassica napus L.) to varying boron availabilities

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Abstract

Backgrounds and aims

Boron (B) is a micronutrient essential for plant normal growth and development. Brassica napus L., one of the leading oil-crop species, is extremely susceptible to deficient and excessive B stresses. Previous studies were concentrated only on the responses of plants to a single B stress only. Here, we aim to provide a comprehensive genome-scale mRNA transcriptomic response of Brassica napus to B deficiency and toxicity.

Methods

The genome-scale mRNA transcriptome was identified and characterized in the leaves and roots of B. napus exposed to B deficiency and toxicity using the digital gene expression (DGE) high-throughput sequencing platforms.

Results

Under both B deficiency and B toxicity, the biomasses, B contents and photosynthetic pigments of B. napus were significantly reduced whereas the anthocyanins were greatly increased. The DGE profiling revealed a total of 3798 and 3048 genes differentially expressed under B deficiency and toxicity, respectively. Transcriptional down- or up-regulation of the B transporter genes, including BnaBOR1s, BnaBOR2, BnaBOR4s and B channel genes, such as BnaNIPs, BnaTIPs and BnaPIPs, may be indispensable for B homeostasis through the molecular modulation of efficient B uptake, transport, distribution and compartmentation. Transcriptional regulation of hydrolytic enzymes and arabinogalactan-protein genes may contribute to the maintenance of cell wall structure and plasma membrane integrity. Transcriptional modulation of antioxidant enzyme genes was likely to balance the reactive oxygen species of plants under B deficiency and toxicity.

Conclusions

Our results provide comprehensive insights into the mRNA transcriptome of key components involved in the B homeostasis network in B. napus and enrich our understanding of the molecular mechanisms by which plants adapt to deficient and excessive B conditions.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (Grant Nos. 31572185 and 31372129) and the National Key Research and Development Program of China (Grant No. 2016YFD0100700).

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  1. National Key Laboratory of Crop Genetic Improvement and Microelement Research Centre, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Yingpeng Hua, Yingna Feng, Ting Zhou & Fangsen Xu

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  1. Yingpeng Hua
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Hua, Y., Feng, Y., Zhou, T. et al. Genome-scale mRNA transcriptomic insights into the responses of oilseed rape (Brassica napus L.) to varying boron availabilities. Plant Soil 416, 205–225 (2017). https://doi.org/10.1007/s11104-017-3204-2

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