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Changes in volatiles in carrots inoculated with ACC deaminase-producing bacteria isolated from organic crops

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An Erratum to this article was published on 30 March 2016

Abstract

Aims

Bacterial ACC deaminase is one of the key tools to ameliorate plant stress by lowering ethylene level in plants. The effects of ACC deaminase-producing bacteria on the volatile profiles in plants have not been examined to date. To address this, we performed metabolic profiling of volatiles in carrots following inoculation of the bacteria producing ACC deaminase.

Methods

We isolated ACC deaminase-producing bacteria from the inner part of the fruits and vegetables grown on organic farms by culturing on ACC-containing media, and screened them with PCR for the acdS gene, mungbean growth assay, and in vitro ACC deaminase activity. The isolated endophytes were evaluated for their ability to alter volatile profiles in carrots.

Results

Eleven bacterial strains possessing the activity to cleave ACC were selected among the 60 isolates grown on the medium containing ACC as a sole N source. Three of them that belonged to Pseudomonas could reduce the levels of (E)-2-hexenal and the other green leaf volatiles (GLVs) and terpenoids in the carrot leaves following inoculation of the seeds.

Conclusions

The isolated endophytes with ACC deaminase activity could alter the composition of volatiles in plants, probably through lowering ethylene level in the plant.

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Acknowledgments

We thank Messrs. Minoru Yamamoto of Organic Farm Tsukuba and Rikio Hashimoto for providing their organic vegetables, and Dr. Tsutae Ito of NARO Institute of Fruit Tree Science for providing various apple products. We also thank Drs. Takayuki Mitsunaga and Toshiro Matsunaga of NARO Agricultural Research Center for their support with statistical analysis and valuable discussions, respectively. We gratefully acknowledge the assistance of experiments by Ms. Setsuko Tanaka and Ms. Yumiko Sasaki. This work was supported by Japan Society for the promotion of Science (JSPS) KAKENHI Grant Number 25660033.

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Author notes

  1. Junko Terakado-Tonooka

    Present address: Saga University, 1 Honjo-machi, Saga, 840-8502, Japan

Authors and Affiliations

  1. NARO Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, 305-8666, Japan

    Hiroaki Matsuoka, Yoshinari Ohwaki, Junko Terakado-Tonooka & Fukuyo Tanaka

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  1. Hiroaki Matsuoka
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  2. Yoshinari Ohwaki
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Correspondence to Fukuyo Tanaka.

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Responsible Editor: Eric J.W. Visser.

An erratum to this article can be found at http://dx.doi.org/10.1007/s11104-016-2866-5.

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Matsuoka, H., Ohwaki, Y., Terakado-Tonooka, J. et al. Changes in volatiles in carrots inoculated with ACC deaminase-producing bacteria isolated from organic crops. Plant Soil 407, 173–186 (2016). https://doi.org/10.1007/s11104-015-2769-x

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