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Differential gene expression of rice roots inoculated with the diazotroph Herbaspirillum seropedicae

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Abstract

Background and aims

Rice (Oryza sativa L.) is the primary source of carbohydrate for the majority of the World's population. Herbaspirillum seropedicae is a diazotroph that lives within and on the surface of rice roots. It can promote the growth of rice, partly by supplying it with fixed nitrogen.

Methods

To better understand the rice–H. seropedicae interaction, cDNA libraries from rice roots either inoculated (RRCH) or uninoculated (RRSH) with the diazotroph were obtained and analysed.

Results

Potential differentially expressed genes identified from the libraries encoded a metallothionein-like protein type 1, a NOD26-like membrane integral protein ZmNIP2-1, a thionin family protein, an oryzain gamma chain precursor, stress-associated protein 1 (OsISAP1), probenazole-inducible protein PBZ1 and auxin- and ethylene-responsive genes. Differential expression was analysed by qRT-PCR for some of these genes and confirmed in most cases. The expression of stress- and defence-related genes coding for thionins, PBZ1 and OsISAP1 was repressed, while expression of a metallothionein gene was induced by inoculation with H. seropedicae. In contrast, expression of auxin-responsive genes was repressed, while expression of ethylene genes was either repressed or induced. The possible involvement of these and other genes in plant-bacterial interactions is discussed.

Conclusions

The decrease in expression of the defence-related proteins PBZ1 and thionins in the rice–H. seropedicae association, suggests that the bacteria modulate plant defence responses during colonisation. The expression of genes responsive to auxin and ethylene also appears to be regulated by the bacteria.

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Fig. 1a,b
Fig. 2a,b
Fig. 3a–c

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Abbreviations

EST:

Expressed sequence tag

PGPR:

Plant-growth promoting rhizobacteria

PRR:

Pattern recognition receptor

PAMP:

Pathogen-associated molecular pattern

RAP-DB:

Rice annotation project-database

GO:

Gene ontology

NBS:

Nucleotide-binding site

ERF:

Ethylene response factor

ARF:

Auxin response factor

PBZ1:

Probenazole-inducible gene

OsISAP1:

Ozyza sativa subspecies indica stress-associated protein

EAS:

5-Epi-aristolochene synthase

RLK:

Receptor-like protein kinase

T3SS:

Type three secretion system

IAA:

Indole acetic acid

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Acknowledgments

We are grateful to Roseli Prado and Julieta Pie for technical support, Embrapa Arroz Feijão and Instituto Riograndense do Arroz for providing seeds. We also thank Euan K. James for revising the final version of this manuscript. This work was supported by CNPq-Instituto do Milênio, INCT-FBN, CNPq and CAPES.

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Authors and Affiliations

  1. Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba, PR, Brazil

    F. Pacheco, R. A. Monteiro, L. M. Cruz, V. A. Baura, F. O. Pedrosa & E. M. Souza

  2. Departamento de Genética, Universidade Federal do Paraná, Rua Coronel Francisco Heráclito dos Santos, C. Postal 19071, 81531-980, Curitiba, PR, Brazil

    L. C. C. Brusamarello-Santos & R. Wassem

  3. Apollo Bramwell Hospital, Royal Road, Moka, Mauritius

    S. M. M. Aljanabi

Authors
  1. L. C. C. Brusamarello-Santos
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  2. F. Pacheco
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  3. S. M. M. Aljanabi
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  4. R. A. Monteiro
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  5. L. M. Cruz
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  6. V. A. Baura
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  7. F. O. Pedrosa
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  8. E. M. Souza
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  9. R. Wassem
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Correspondence to R. Wassem.

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Responsible Editor: Euan K. James.

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Brusamarello-Santos, L.C.C., Pacheco, F., Aljanabi, S.M.M. et al. Differential gene expression of rice roots inoculated with the diazotroph Herbaspirillum seropedicae . Plant Soil 356, 113–125 (2012). https://doi.org/10.1007/s11104-011-1044-z

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  • DOI: https://doi.org/10.1007/s11104-011-1044-z

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