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Soil microbial community structure in European forests in relation to forest type and atmospheric nitrogen deposition

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Abstract

The objective of the present study was to evaluate the combined effect of vegetation and N deposition on microbial community composition in forest soils. For this, microbial biomass and community structure were assessed by ester linked fatty acid methyl ester (EL-FAME) analyses for 12 European forest sites representing different forest types (coniferous/deciduous) and differing in annual N loads (2–40 kg N ha−1). Microbial community composition was affected by vegetation as indicated by a higher proportion of the marker for arbuscular mycorrhiza (AM) fungi—16:1 11ω—in deciduous forest soils (1.2%–5.7% of total EL-FAMEs) compared to acidic coniferous forest soils (0.5%–1.6%). The two pine forest sites investigated showed the highest proportion of fungi (up to 28% of total EL-FAMEs) and the lowest proportions of Gram-negative and Gram-positive bacteria of all study sites. Nitrogen deposition rates were highly correlated with the ratios of cyclopropyl fatty acids to their precursors (r = 0.82; P < 0.01) and of bacteria to fungi (r = 0.71; P < 0.05). The two sites with the highest N deposition (≥32.3 kg N ha−1a−1) were depleted in the marker fatty acids for AM fungi and other fungi. Our findings suggest that vegetation has a pronounced effect on microbial community structure, but this effect is masked by high N inputs (>30 kg N ha−1a−1).

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Acknowledgements

We thank Klaus Butterbach-Bahl for excellent project coordination. We thank Brigitte Schraufstädter and Veronika Bendl for careful laboratory analysis, Barbara Kitzler for field work, and the NOFRETETE team for soil sampling. The work was funded by the European Commission in the NOFRETETE project (EVK2-CT2001-00106) of the fifth framework program and co-funded by the NitroEurope Integrated Project (017841) of the sixth framework program.

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  1. Soil Biology, Federal Research and Training Centre for Forests, Natural Hazards and Landscape (BFW), Seckendorff-Gudent Weg 8, 1131, Vienna, Austria

    Sophie Zechmeister-Boltenstern, Kerstin Michel & Michael Pfeffer

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  1. Sophie Zechmeister-Boltenstern
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  2. Kerstin Michel
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  3. Michael Pfeffer
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Correspondence to Kerstin Michel.

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Zechmeister-Boltenstern, S., Michel, K. & Pfeffer, M. Soil microbial community structure in European forests in relation to forest type and atmospheric nitrogen deposition. Plant Soil 343, 37–50 (2011). https://doi.org/10.1007/s11104-010-0528-6

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