Abstract
To increase grain quality, optimal nutrient management is desirable. Recently, atmospheric S emissions from industry have decreased leading to reduced grain quality. Fertilization of N primarily affects protein concentration and biomass production while S fertilization affects the fine tuning of storage protein composition. Additionally, high N supply increases the N/S ratio to the extent that the grains could be classified as S-deficient. Although total protein amounts were not changed by the fertilizer treatments, the amounts of individual gluten proteins were altered. High resolution proteomic analysis of S-containing gluten protein fractions demonstrates that under high N supply, low or high S fertilization significantly changed 41 and 66 of the gliadin and the glutenin proteins. In particular, this change may lead to different dough and baking quality. In conclusion, a high N fertilization induces S deficiency together with a change in gluten proteins and a loss of nutritional quality of grains.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
De Ruiter JM, Martin RJ (2001) Management of nitrogen and sulphur fertiliser for improved bread wheat (Triticum aestivum) quality. NZ J Crop Hortic Sci 29:287–299
Ewart JAD (1978) Glutamin and dough tenacity. J Sci Food Agric 29:551–556
Görg A, Weiss W (1998) High-resolution two-dimensional electrophoresis of proteins using immobilized pH gradients. In: Celis J (ed) Cell Biology. A Laboratory Handbook. Academic Press NY, pp 386–397
Granvogl M, Wieser H, Koehler P, von Tucher S, Schieberle P (2008) Influence of sulfur fertilization on the amounts of free amino acids in wheat. Correlation with baking properties as well as with 3-aminoproprionamide and acrylaminde generation during baking. J Agric Food Chem 55:4271–4277
Haneklaus S, Murpy DLP, Nowak G, Schnug E (1995) Effects of the timing of sulphur application on grain yield and yield components of wheat. Z Pflanzenernähr Bodenk 158:83–85
Jahn-Deesbach W, Marquard R, Schipper A (1970) Untersuchungen über den Einfluß von Sorte und Stickstoffdüngung auf die Eiweißfraktionen von Weizen und Gerste. Z Acker- und Pflanzenbau 132:151–162
Kopriva S, Rennenberg H (2004) Control of sulphate assimilation and glutathione synthesis: interaction with N and C metabolism. J Exp Bot 55:1831–1842
Lerner SE, Seghezzo ML, Molfese ER, Ponzio NR, Cogliatti M, Rogers WJ (2006) N- and S-fertilizer effects on grain composition, industrial quality and end-use in durum wheat. J Cereal Sci 44:2–11
Martre P, Porter JR, Jamieson PD, Triboï E (2003) Modeling grain nitrogen accumulation and protein composition to understand the sink/source regulations of nitrogen remobilization for wheat. Plant Physiol 133:1959–1967
Moss HJ, Wrigley CW, MacRitchie F, Randall PJ (1981) Sulfur and Nitrogen fertilizer effects on wheat. II. Influence on grain quality. Austral J Agric Res 32:213–2276
Randall PJ, Wrigley CW (1986) Effects of sulphur supply on the yield, composition, and quality of grain from cereals, oilseeds and legumes. Adv Cereal Sci Technol 8:171–206
Salvagiotti F, Miralles DJ (2008) Radiation interception, biomass production and grain yield as affected by the interaction of nitrogen and sulphur fertilization in wheat. Europ J Agronomy 28:282–290
Sarandon SJ, Gianibelli MC (1990) Effect of foliar urea spraying and nitrogen application at sowing upon dry matter and nitrogen distribution in wheat (Triticum aestivum L). Agronomie 10:183–189
Shewry PR, Halford NG (2002) Cereal seed storage proteins: structures, properties and role in grain utlization. J Exp Bot 53:947–958
Strong WM (1982) Effect of late application of nitrogen on the yield and protein content of wheat. Aust J Exp Agric Anim Husb 22:54–61
Tea I, Genter T, Naulet N, Marie LM, Kleiber D (2007) Interaction between nitrogen and sulfur by foliar application and its effects on flour bread-making quality. J Sci Food Agric 87:2853–2859
Van Bel AJE, Offler CE, Patrick JW (2003) Sources and sinks. In: Van Bel AJE (ed) Photosynthesis and partitioning / Sources and sinks. Elsevier
Westermeier R, Naven T, Höpker HR (2008) Proteomics in practice. A guide to successful experimental design, Sec. Ed. Wiley-VHC, Verlag Weinheim, Germany
Wieser H, Seilmeier W (1998) The influence of nitrogen fertilization on quantities and proportions of different protein types in wheat flour. J Sci Food Agric 76:49–55
Wieser H, Gutser R, von Tucher S (2004) Influence of sulphur fertilization on quantities and proportions of gluten protein types in wheat flour. J Cereal Sci 40:239–244
Wooding AR, Kavale S, MacRitchie F, Stoddard FL, Wallace A (2000) Effects of nitrogen and sulfur fertilizer on protein composition, mixing requirements, and dough strength of flour wheat cultivars. Cereal Chem 77:798–807
Wrigley CW, du Cros DL, Archer MJ, Downie PG, Roxburgh CM (1980) The sulfur content in wheat endosperm and its relevance to grain quality. Aust J Plant Physiol 7:755–766
Zhao FJ, Hawkesford MJ, McGrath SP (1999) Sulphur assimilation and effects on yield and quality of wheat. J Cereal Sci 30:1–17
Zörb C, Schmitt S, Neeb A, Karl S, Linder M, Schubert S (2004) The biochemical reaction of maize (Zea mays L.,) to salt is characterized by a mitigation of symptoms and not by a specific adaptation. Plant Sci 167:91–100
Acknowledgements
We thank Mrs. Christa Lein for excellent technical assistance.
Author information
Authors and Affiliations
Corresponding author
Additional information
Responsible Editor: Ismail Cakmak.
Dedicated to the occasion of 80th birthday of Prof. Dr. Drs. h. c. Konrad Mengel
Electronic Supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Zörb, C., Grover, C., Steinfurth, D. et al. Quantitative proteome analysis of wheat gluten as influenced by N and S nutrition. Plant Soil 327, 225–234 (2010). https://doi.org/10.1007/s11104-009-0049-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11104-009-0049-3