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Communities of arbuscular mycorrhizal fungi and bacteria in the rhizosphere of Caragana korshinkii and Hippophae rhamnoides in Zhifanggou watershed

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Abstract

The process of ecological restoration and reconstruction in Zhifanggou watershed forms a special ecosystem on the Loess Plateau. Little is known about the communities of arbuscular mycorrhizal fungi (AMF) and bacteria in this ecosystem. The aim of this study was to analyze the communities of AMF and bacteria, and their relationship in the rhizosphere of Caragana korshinkii and Hippophae rhamnoides in Zhifanggou watershed. Soil samples were collected from Zhifanggou watershed. The communities of AMF and bacteria were analyzed by using nested PCR of rDNA fragments and denaturing gradient gel electrophoresis (DGGE). Diversity analysis revealed that the bacterial Shannon diversity index was higher than that of AMF, and the AMF and bacterial Shannon diversity index in the rhizosphere of H. rhamnoides was higher than that of C. korshinkii. Principal component analysis (PCA) revealed that host plant had a significant influence on the bacterial community structure, but no strict specificity with AMF. Correlation analysis showed that the AMF communities had a significant positive correlation with the bacterial communities, and that indicated a significant effect of AMF on bacteria.

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Abbreviations

AMF:

arbuscular mycorrhizal fungi

C:

Caragana korshinkii

DGGE:

denaturing gradient gel electrophoresis

H:

Hippophae rhamnoides

PCA:

principal component analysis

PCR:

polymerase chain reaction

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Acknowledgements

We acknowledge the financial support of the Key Project of National Natural Science Foundation of China (30630054, 30730073), the Program for Changjiang Scholars and Innovative Research Team in University of China (IRT0748). We thank Jingjiang Hu (Northwest A&F University) for his comments and discussion for some of the ideas presented in this paper. We also thank anonymous reviewers for their valuable suggestions that enhanced the manuscript.

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  1. College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, China

    Haoqiang Zhang & Yaoqin Xue

  2. College of Forestry, Northwest A&F University, Yangling, Shaanxi, 712100, China

    Ming Tang, Hui Chen & Ye Feng

  3. Foreign Language Department, Northwest A&F University, Yangling, Shaanxi, 712100, China

    Zhiqiang Tian

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  1. Haoqiang Zhang
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Correspondence to Ming Tang.

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Zhang, H., Tang, M., Chen, H. et al. Communities of arbuscular mycorrhizal fungi and bacteria in the rhizosphere of Caragana korshinkii and Hippophae rhamnoides in Zhifanggou watershed. Plant Soil 326, 415–424 (2010). https://doi.org/10.1007/s11104-009-0022-1

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