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Glyphosate-induced impairment of plant growth and micronutrient status in glyphosate-resistant soybean (Glycine max L.)

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Abstract

This investigation demonstrated potential detrimental side effects of glyphosate on plant growth and micronutrient (Mn, Zn) status of a glyphosate-resistant (GR) soybean variety (Glycine max cv. Valiosa), which were found to be highly dependent on the selected growth conditions. In hydroponic experiments with sufficient Mn supply [0.5 μM], the GR cv. Valiosa produced similar plant biomass, root length and number of lateral roots in the control treatment without glyphosate as compared to its non-GR parental line cv. Conquista. However, this was associated with 50% lower Mn shoot concentrations in cv. Conquista, suggesting a higher Mn demand of the transgenic cv. Valiosa under the selected growth conditions. Glyphosate application significantly inhibited root biomass production, root elongation, and lateral root formation of the GR line, associated with a 50% reduction of Mn shoot concentrations. Interestingly, no comparable effects were detectable at low Mn supply [0.1 μM]. This may indicate Mn-dependent differences in the intracellular transformation of glyphosate to the toxic metabolite aminomethylphosphonic acid (AMPA) in the two isolines. In soil culture experiments conducted on a calcareous loess sub-soil of a Luvisol (pH 7.6) and a highly weathered Arenosol (pH 4.5), shoot biomass production and Zn leaf concentrations of the GR-variety were affected by glyphosate applications on the Arenosol but not on the calcareous Loess sub-soil. Analysis of micronutrient levels in high and low molecular weight (LMW) fractions (80% ethanol extracts) of young leaves revealed no indications for internal immobilization of micronutrients (Mn, Zn, Fe) by excessive complexation with glyphosate in the LMW phase.

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Abbreviations

AMPA:

aminomethylphosphonic acid

cv.:

cultivar

GM:

genetically modified

GR:

glyphosate-resistant

LMW:

low molecular weight

HMW:

high molecular weight

References

  • Arregui MC, Lenardo’n A, Sanchez D, Maitre MI, Scotta R, Enrique S (2003) Monitoring glyphosate residues in transgenic glyphosate-resistant soybean. Pest Manage Sci 60:163–166

    Article  CAS  Google Scholar 

  • Bailey WA, Poston DH, Wilson HP, Hines TE (2002) Glyphosate interactions with manganese. Weed Technol 16:792–799 doi:10.1614/0890-037X(2002)016[0792:GIWM]2.0.CO;2

    Article  CAS  Google Scholar 

  • Baylis AD (2000) Why glyphosate is a global herbicide: strengths, weaknesses and prospects. Pest Manag Sci 56:299–308 doi:10.1002/(SICI)1526-4998(200004)56:4<299::AID-PS144>3.0.CO;2-K

    Article  CAS  Google Scholar 

  • Bennett WF (1993) Nutrient deficiencies and toxicities in crop plants. APS Press, St Paul

    Google Scholar 

  • Bernards ML, Thelen KD, Penner D (2005a) Glyphosate efficacy is antagonized by manganese. Weed Technol 19:27–34 doi:10.1614/WT-03-193R2

    Article  CAS  Google Scholar 

  • Bernards ML, Thelen KD, Muthukumaran RB, Penner D, McCracken JL (2005b) Glyphosate interaction with manganese in tank mixtures and its effect on glyphosate absorption and translocation. Weed Sci 53:787–794 doi:10.1614/WS-05-043R.1

    Article  CAS  Google Scholar 

  • Cakmak I (2000) Possible roles of zinc in protecting plant cells from damage by reactive oxygen species. New Phytol 146:185–205 doi:10.1046/j.1469-8137.2000.00630.x

    Article  CAS  Google Scholar 

  • Cerdeira AL, Duke SO (2006) The current status and environmental impacts of glyphosate-resistant crops: a review. J Environ Qual 35:1633–1658 doi:10.2134/jeq2005.0378

    Article  PubMed  CAS  Google Scholar 

  • de María N, de Felipe MR, Fernández-Pascual M (2005) Alterations induced by glyphosate on lupin photosynthetic apparatus and nodule ultrastructure and some oxygen diffusion related proteins. Plant Physiol Biochem 43:985–996 doi:10.1016/j.plaphy.2005.09.001

    Article  PubMed  CAS  Google Scholar 

  • Duke SO (1988) Glyphosate. In: Kearney PC, Kaufman DD (eds) Herbicides: chemistry, degradation, and mode of action, vol. 3. Dekker, New York, pp 1–70

    Google Scholar 

  • Duke SO, Rimando AM, Pace PF, Reddy KN, Smeda RJ (2003) Isoflavone, glyphosate, and aminomethylphosphonic acid levels in seeds of glyphosate-treated, glyphosate-resistant soybean. J Agric Food Chem 51:340–344 doi:10.1021/jf025908i

    Article  PubMed  CAS  Google Scholar 

  • Eker S, Levent O, Yazici A, Erenoglu B, Römheld V, Cakmak I (2006) Foliar-applied glyphosate substantially reduced uptake and transport of iron and manganese in sunflower (Helianthus annuus L.). Plants J Agric Food Chem 54:10019–10025 doi:10.1021/jf0625196

    Article  CAS  Google Scholar 

  • Feng PCC, Pratley JE, Bohn JA (1999) Resistance to glyphosate in Lolium rigidum. II. Uptake, translocation and metabolism. Weed Sci 47:412–415

    CAS  Google Scholar 

  • Gauvrit C, Gaudry JC, Lucotte T, Cabanne F (2001) Biological evidence for a 1:1 Ca2+: glyphosate association in deposit residuals of the leaf surface of barley. Weed Res 41:433–455 doi:10.1046/j.1365-3180.2001.00248.x

    Article  CAS  Google Scholar 

  • Geiger DR, Kapitan SW, Tucci MA (1986) Glyphosate inhibits photosynthesis and allocation of carbon to starch in sugar beet leaves. Plant Physiol 82:468–472

    Article  PubMed  CAS  Google Scholar 

  • Giesy JP, Dobson S, Solomon KR (2000) Ecotoxilogical risk assessment for Roundup® herbicide. Rev Environ Contam Toxicol 167:35–120

    CAS  Google Scholar 

  • Gordon B (2007) Manganese nutrition of glyphosate-resistant and conventional soybeans. Better Crops 91/4:12–13

    Google Scholar 

  • Hernandez A, Garcia-Plazaola JI, Becerril JM (1999) Glyphosate effects on phenolic metabolism of nodulated soybean (Glycine max L. Merr.). J Agric Food Chem 47:2920–2925 doi:10.1021/jf981052z

    Article  PubMed  CAS  Google Scholar 

  • Hetherington PR, Reynolds TL, Marshall G, Kirkwood RC (1999) The absorption, translocation and distribution of the herbicide glyphosate in maize expressing the CP-4 transgene. J Exp Bot 50:1567–1576 doi:10.1093/jexbot/50.339.1567

    Article  CAS  Google Scholar 

  • Huber DM (2006) Strategies to ameliorate glyphosate immobilization of manganese and its impact on the rhizosphere and disease. In: Lorenz N, Dick R (eds) Proceedings of the glyphosate potassium symposium 2006. Ohio State University, AG Spectrum, DeWitt, Iowa

    Google Scholar 

  • Huber DM, McCay-Buys TS (1993) A multiple component analysis of the take-all disease of cereals. Plant Dis 77:437–447

    Google Scholar 

  • Jolley VD, Hansen NC, Shiffler AK (2004) Nutritional and management related interactions with iron-deficiency stress response mechanisms. Soil Sci Plant Nutr 50:973–981

    CAS  Google Scholar 

  • Komossa D, Gennity I, Sandermann H (1992) Plant metabolism of herbicides with C–P bonds: glyphosate. Pestic Biochem Physiol 43:85–94 doi:10.1016/0048-3575(92)90022-R

    Article  CAS  Google Scholar 

  • Nandula VK, Reddy KN, Rimando AM, Duke SO, Poston DH (2007) Glyphosate-resistant and susceptible soybean (Glycine max) and canola (Brassica napus). Dose response and metabolism relationships with glyphosate. J Agric Food Chem 55:3540–3545 doi:10.1021/jf063568l

    Article  PubMed  Google Scholar 

  • Neumann G, Kohls S, Landsberg E, Stock-Oliveira Souza K, Yamada T, Römheld V (2006) Relevance of glyphosate transfer to non-target plants via the rhizosphere. J Plant Dis Protect 20:963–969

    Google Scholar 

  • Ozturk L, Yaciki A, Eker S, Gokmen O, Römheld V, Cakmak I (2008) Glyphosate inhibition of ferric reductase activity in iron-deficient sunflower roots. New Phytol 177:899–906 doi:10.1111/j.1469-8137.2007.02340.x

    Article  PubMed  CAS  Google Scholar 

  • Reddy KN, Rimando AM, Duke SO (2004) Aminomethylphosphonic acid, a metabolite of glyphosate, causes injury in glyphosate-treated, glyphosate-resistant soybean. J Agric Food Chem 52:5139–5143 doi:10.1021/jf049605v

    Article  PubMed  CAS  Google Scholar 

  • Reuter DJ, Robinson JB (1997) Plant analysis—an interpretation manual. CSRIO, Collingwood

    Google Scholar 

  • Schönherr J, Schreiber L (2004) Interactions of calcium ions with weakly acidic ingredients slow cuticular penetration: a case study with glyphosate. J Agric Food Chem 51:6546–6551 doi:10.1021/jf049500s

    Article  CAS  Google Scholar 

  • Service RF (2007) A growing threat down on the farm. Science 316(5828):1114–1117 doi:10.1126/science.316.5828.1114

    Article  PubMed  CAS  Google Scholar 

  • Sprankle P, Meggitt WF, Penner D (1975) Adsorption, action and translocation of glyphosate. Weed Sci 23:235–240

    CAS  Google Scholar 

  • VDLUFA (2004) Bestimmung von Magnesium, Natrium und den Spurennährstoffen Kupfer, Mangan, Zink, und Bor im Calciumchlorid/DTPA-Auszug. VDLUFA-Methodenbuch I, A 6.4.1.,VDLUFA-Verlag, Darmstadt, Germany

  • Wagner R, Kogan M, Parada AM (2003) Phytotoxic activity of root absorbed glyphosate in corn seedlings (Zea mays L.). Weed Biol Manag 3:228–232 doi:10.1046/j.1444-6162.2003.00110.x

    Article  CAS  Google Scholar 

  • Wang YJ, Zhou DM, Sun RJ, Jia DA, Zhu HW, Wang SQ (2008) Zinc adsorption on goethite as affected by glyphosate. J Hazard Mater 151:179–184 doi:10.1016/j.jhazmat.2007.05.060

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institut für Pflanzenernährung (330), Universität Hohenheim, 70593, Stuttgart, Germany

    Sebastian Bott, Tsehaye Tesfamariam, Volker Römheld & Günter Neumann

  2. Sabanci University, 8174 Tuzla, Istanbul, Turkey

    Hande Candan & Ismail Cakmak

Authors
  1. Sebastian Bott
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  2. Tsehaye Tesfamariam
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  3. Hande Candan
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  4. Ismail Cakmak
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  5. Volker Römheld
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  6. Günter Neumann
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Correspondence to Günter Neumann.

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Responsible Editor: Petra Marschner.

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Bott, S., Tesfamariam, T., Candan, H. et al. Glyphosate-induced impairment of plant growth and micronutrient status in glyphosate-resistant soybean (Glycine max L.). Plant Soil 312, 185–194 (2008). https://doi.org/10.1007/s11104-008-9760-8

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  • DOI: https://doi.org/10.1007/s11104-008-9760-8

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