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Isolation and characterization of phosphate solubilizing rhizobacteria to improve plant health of tomato

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Abstract

In the present study, 43 isolates of Phosphate solubilizing rhizobacteria (PSRB) were isolated from 37 rhizospheric soil samples of tomato collected from tomato growing regions of Karnataka. Among the 43 isolates, 33 were found to be positive for solubilizing both inorganic and organic forms of phosphorous. The isolates were analyzed for their ability to colonize roots of tomato and to increase the seed quality parameters under laboratory conditions. On the basis of above criteria, 16 isolates were selected for further studies. Organic acids from PSRB isolates were analyzed and phytase zymogram for two isolates viz., PSRB21 and 31 was prepared. Under greenhouse conditions, all selected isolates showed increased shoot length, root length, fresh weight, dry weight and phosphorous content of tomato seedlings to various extent with respect to control. Analysis of pH and available phosphorous in rhizosphere soil samples of 30 day-old-seedlings revealed that the available phosphorous content was high in rhizospheric soil samples of plants raised from seeds bacterized with PSRB isolates over control. Even though all selected PSRB’s were able to increase the plant growth, only few of them showed protection against fusarium wilt and none of them against early blight.

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Acknowledgements

The present work is the result of major research project entitled, “A novel strain of PGPR as biopesticide for the improvement plant health in vegetables” awarded by University Grants Commission, Government of India, New Delhi, India. The authors are grateful to Dr. R. Suma, Subject Matter Specialist (SS and AC), Krishi Vignayan Kendra, V. C. Farm, Mandya – 571 405, Karnataka, India for her valuable help in the present study. The author wish to thank the Chairman, Department of Applied Botany, Seed Pathology and Biotechnology, University of Mysore, Manasagangotri, MYSORE 570 006, India.

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  1. Department of Applied Botany, Seed Pathology and Biotechnology, University of Mysore, Manasagangotri, Mysore, 570 006, Karnataka, India

    P. Hariprasad & S. R. Niranjana

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  1. P. Hariprasad
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  2. S. R. Niranjana
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Correspondence to S. R. Niranjana.

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Hariprasad, P., Niranjana, S.R. Isolation and characterization of phosphate solubilizing rhizobacteria to improve plant health of tomato. Plant Soil 316, 13–24 (2009). https://doi.org/10.1007/s11104-008-9754-6

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