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Effect of arsenic–phosphorus interaction on arsenic-induced oxidative stress in chickpea plants

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Abstract

Arsenic-induced oxidative stress in chickpea was investigated under glasshouse conditions in response to application of arsenic and phosphorus. Three levels of arsenic (0, 30 and 60 mg kg−1) and four levels of P (50, 100, 200, and 400 mg kg−1) were applied to soil-grown plants. Increasing levels of both arsenic and P significantly increased arsenic concentrations in the plants. Shoot growth was reduced with increased arsenic supply regardless of applied P levels. Applied arsenic induced oxidative stress in the plants, and the concentrations of H2O2 and lipid peroxidation were increased. Activity of superoxide dismutase (SOD) and concentrations of non-enzymatic antioxidants decreased in these plants, but activities of catalase (CAT) and ascorbate peroxidase (APX) were significantly increased under arsenic phytotoxicity. Increased supply of P decreased activities of CAT and APX, and decreased concentrations of non-enzymatic antioxidants, but the high-P plants had lowered lipid peroxidation. It can be concluded that P increased uptake of arsenic from the soil, probably by making it more available, but although plant growth was inhibited by arsenic the P may have partially protected the membranes from arsenic-induced oxidative stress.

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Abbreviations

AA:

Non enzymatic antioxidant activity

APX:

Ascorbate peroxidase

CAT:

Catalase

MDA:

Malondialdehyde

NBT:

Nitroblue tetrazolium

PEDXRF:

Polarized energy dispersive X-ray fluorescence

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

XRF:

X-ray fluorescence

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Authors and Affiliations

  1. Faculty of Agriculture, Department of Soil Science and Plant Nutrition, Ankara University, 06110, Ankara, Turkey

    Aydin Gunes & Ali Inal

  2. Institute of Integrative and Comparative Biology, University of Leeds, Leeds, LS2 9JT, UK

    David J. Pilbeam

Authors
  1. Aydin Gunes
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  2. David J. Pilbeam
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  3. Ali Inal
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Correspondence to Aydin Gunes.

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Responsible Editor: A.C. Borstlap.

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Gunes, A., Pilbeam, D.J. & Inal, A. Effect of arsenic–phosphorus interaction on arsenic-induced oxidative stress in chickpea plants. Plant Soil 314, 211–220 (2009). https://doi.org/10.1007/s11104-008-9719-9

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  • DOI: https://doi.org/10.1007/s11104-008-9719-9

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