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Cadmium-induced oxidative damage in rice leaves is reduced by polyamines

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Abstract

The protective effect of polyamines against Cd toxicity of rice (Oryza sativa) leaves was investigated. Cd toxicity to rice leaves was determined by the decrease in protein content. CdCl2 treatment results in (1) increased Cd content, (2) induction of Cd toxicity, (3) increase in H2O2 and malondialdehyde (MDA) contents, (4) decrease in ascorbic acid (ASC) and reduced glutathione (GSH) contents, and (5) increase in the activities of antioxidative enzymes (superoxide dismutase, glutathione reductase, ascorbate peroxidase, catalase, and peroxidase). Spermidine (Spd) and spermine (Spm), but not putrescine (Put), were effective in reducing CdCl2-induced toxicity. Spd and Spm prevented CdCl2-induced increase in the contents of H2O2 and MDA, decrease in the contents of ASC and GSH, and increase in the activities of antioxidative enzymes. Spd and Spm pretreatments resulted in a decrease in Cd content when compared with H2O pretreatment, indicating that Spd and Spm may reduce the uptake of Cd. Results of the present study suggest that Spd and Spm are able to protect Cd-induced oxidative damage and this protection is most likely related to the avoidance of H2O2 generation and the reduction of Cd uptake.

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Abbreviations

APX:

Ascorbate peroxidase

ASC:

Ascorbic aicd

CAT:

Catalase

DAB:

3,3′-Diaminobenzidine

DHA:

Dehydroascorbate

DW:

Dry weight

FW:

Fresh weight

GR:

Glutathione reductase

GSH:

Reduced glutathione

GSSG:

Oxidized glutathione

POX:

Peroxidase

Put:

Putrescine

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

Spd:

Spermidine

Spm:

Spermine

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Acknowledgements

This work was supported by a research grant from the National Science Council of the Republic of China (NSC 95-2313-B002-007).

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  1. Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China

    Yi Ting Hsu & Ching Huei Kao

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  1. Yi Ting Hsu
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Correspondence to Ching Huei Kao.

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Hsu, Y.T., Kao, C.H. Cadmium-induced oxidative damage in rice leaves is reduced by polyamines. Plant Soil 291, 27–37 (2007). https://doi.org/10.1007/s11104-006-9171-7

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