Abstract
In order to improve the basis for utilising nitrogen (N) fixed by white clover (Trifolium repens L.) in northern agriculture, we studied how defoliation stress affected the N contents of major plant organs in late autumn, N losses during the winter and N accumulation in the following spring. Plants were established from stolon cuttings and transplanted to pots that were dug into the field at Apelsvoll Research Centre (60°42′ N, 10°51′ E) and at Holt Research Centre (69°40′ N, 18°56′ E) in spring 2001 and 2002. During the first growing season, the plants were totally stripped of leaves down to the stolon basis, cut at 4 cm height or left undisturbed. The plants were sampled destructively in late autumn, early spring the second year and after 6 weeks of new spring growth. The plant material was sorted into leaves, stolons and roots. Defoliation regime did not influence the total amount of leaf N harvested during and at the end of the first growing season. However, for intensively defoliated plants, the repeated leaf removal and subsequent regrowth occurred at the expense of stolon and root development and resulted in a 61–85% reduction in the total plant N present in late autumn and a 21–59% reduction in total accumulation of plant N (plant N present in autumn + previously harvested leaf N). During the winter, the net N loss from leaf tissue (N not recovered in living nor dead leaves in the spring) ranged from 57% to 74% of the N present in living leaves in the autumn, while N stored in stolons and roots was much better conserved. However, the winter loss of stolon N from severely defoliated plants (19%) was significantly larger than from leniently defoliated (12%) and non-defoliated plants (6%). Moreover, the fraction of stolon N determined as dead in the spring was 63% for severely defoliated as compared to 14% for non-defoliated plants. Accumulation in absolute terms of new leaf N during the spring was highly correlated to total plant N in early spring (R 2 = 0.86), but the growth rates relative to plant N present in early spring were not and, consequently, were similar for all treatments. The amount of inorganic N in the soil after snowmelt and the N uptake in plant root simulator probes (PRSTM) during the spring were small, suggesting that microbial immobilisation, leaching and gas emissions may have been important pathways for N lost from plant tissue.
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References
H Bauer M Nagele M Comploj V Galler M Mair E Unterpertinger (1994) ArticleTitlePhotosynthesis in cold-acclimated leaves of plants with various degrees of freezing tolerance Physiol. Plant. 91 403–412 Occurrence Handle1:CAS:528:DyaK2cXltVCgsb4%3D Occurrence Handle10.1111/j.1399-3054.1994.tb02967.x
P M Berry R Sylvester-Bradley L Philipps D J Hatch S P Cuttle F W Rayns P Gosling (2002) ArticleTitleIs the productivity of organic farms restricted by the supply of available nitrogen? Soil Use Manag. 18 248–255 Occurrence Handle10.1079/SUM2002129
V Bouchart J H Macduff A Ourry M M Svenning A P Gay J C Simon J Boucaud (1998) ArticleTitleSeasonal pattern of accumulation and effects of low temperatures on storage compounds in Trifolium repens Physiol. Plant. 104 65–74 Occurrence Handle1:CAS:528:DyaK1cXmvFyrtLg%3D Occurrence Handle10.1034/j.1399-3054.1998.1040109.x
T A Breland (1996) ArticleTitleGreen manuring with clover and ryegrass catch crops undersown in small grains: Crop development and yields Acta Agric. Scand., Sec B, Soil Plant Sci. 46 30–40
R W Brougham (1958) ArticleTitleLeaf development in Swards of white clover (Trifolium repens L.) New Zeal J Agr Res 1 707–718
G Carlsson K Huss-Danell (2003) ArticleTitleNitrogen fixation in perennial forage legumes in the field Plant Soil 253 353–372 Occurrence Handle1:CAS:528:DC%2BD3sXlsFKqsr0%3D Occurrence Handle10.1023/A:1024847017371
D F Chapman M J Robson R W Snaydon (1990) ArticleTitleShort-term effects on manipulating the source: sink ratio of white clover (Trifolium repens L.) plants on export of carbon from, and morphology of, developing leaves Physiol. Plant. 80 262–266 Occurrence Handle1:CAS:528:DyaK3MXjvFCgsw%3D%3D Occurrence Handle10.1111/j.1399-3054.1990.tb04406.x
C R Clement M J Hopper L H P Jones E L Leafe (1978) ArticleTitleThe uptake of nitrate by Lolium perenne from flowing nutrient solution. II. Effect of light, defoliation, and relationship of CO2 flux J. Exp. Bot. 29 1173–1183 Occurrence Handle1:CAS:528:DyaE1MXjs1Cktg%3D%3D
R P Collins M J Glendining I Rhodes (1991) ArticleTitleThe relationships between stolon characteristics, winter survival and annual yields in white clover (Trifolium repens L.) Grass Forage Sci. 46 51–61 Occurrence Handle10.1111/j.1365-2494.1991.tb02207.x
W R Cookson I S Cornforth J S Rowarth (2002) ArticleTitleWinter soil temperature (2–15 degrees C) effects on nitrogen transformation in clover green manure amended or unamended soils; a laboratory and field study Soil Biol. Biochem. 34 1401–1415 Occurrence Handle1:CAS:528:DC%2BD38XnsVWrsrs%3D Occurrence Handle10.1016/S0038-0717(02)00083-4
N Corre V Bouchart A Ourry J Boucaud (1996) ArticleTitleMobilization of nitrogen reserves during regrowth of defoliated Trifolium repens L. and identification of potential vegetative storage proteins J. Exp. Bot. 47 1111–1118 Occurrence Handle1:CAS:528:DyaK28XmtVKmur4%3D
R A Culvenor R J Simpson (1991) ArticleTitleMobilization of nitrogen in swards of Trifolium-Subterraneum L. during regrowth after defoliation New Phytol. 117 81–90 Occurrence Handle1:CAS:528:DyaK3MXit1yjsro%3D Occurrence Handle10.1111/j.1469-8137.1991.tb00947.x
S Dalmansdóttir A Helgadóttir B Gudleifsson (2001) ArticleTitleFatty accid and sugar content in white clover in relation to frost tolerance and ice-encasement tolerance Ann. Bot. 88 753–760 Occurrence Handle10.1006/anbo.2001.1465 Occurrence Handle1:CAS:528:DC%2BD3MXnvValuro%3D
P Dorsch A Palojarvi S Mommertz (2004) ArticleTitleOverwinter greenhouse gas fluxes in two contrasting agricultural habitats Nutr. Cycling Agroecosyst. 70 117–133 Occurrence Handle10.1023/B:FRES.0000048473.11362.63
P S Evans (1977) ArticleTitleComparative root morphology of some pasture grasses and clovers N. Z. J. Agric. Res. 20 331–335
J Frame P Newbould (1986) ArticleTitleAgronomy of white clover Adv. Agron. 40 1–88
B F Frankow-Lindberg (2001) ArticleTitleAdaptation to winter stress in nine white clover populations: changes in non-structural carbohydrates during exposure to simulated winter conditions and “spring” regrowth potential Ann. Bot. 88 745–751 Occurrence Handle1:CAS:528:DC%2BD3MXnvValur0%3D Occurrence Handle10.1006/anbo.2001.1432
B F Frankow-Lindberg H A Fircks Particlevon (1998) ArticleTitlePopulation fluctuations in three contrasting white clover cultivars under cutting, with particular reference to overwintering properties J. Agric. Sci. 131 143–153 Occurrence Handle10.1017/S0021859698005693
A J Gordon W Kessler (1990) ArticleTitleDefoliation induced stress in nodules of white clover J. Exp. Bot. 231 1255–1262
E Goulas F Le Dily L Teissedre G Corbel C Robin A Ourry (2001) ArticleTitleVegetative storage proteins in white clover (Trifolium repens L.): Quantitative and qualitative features Ann. Bot. 88 789–795 Occurrence Handle1:CAS:528:DC%2BD3MXnvValurs%3D Occurrence Handle10.1006/anbo.2001.1501
B Hansen E S Kristensen R Grant H Høgh-Jensen S E Simmelsgaard J E Olesen (2000) ArticleTitleNitrogen leaching from conventional versus organic farming systems – a systems modelling approach Eur. J. Agron. 13 65–82 Occurrence Handle1:CAS:528:DC%2BD3cXls1Ogs7k%3D Occurrence Handle10.1016/S1161-0301(00)00060-5
M J M Hay P Nes M R Robertson (1985) ArticleTitleEffect of grazing management and season on nitrogen and phosphorus content of leaves and stolons of white clover in mixed swards N. Z. J. Exp. Agric. 13 209–214
T M Henriksen T A Breland (1999) ArticleTitleDecomposition of crop residues in the field: Evaluation of a simulation model developed from microcosm studies Soil Biol. Biochem. 31 1423–1434 Occurrence Handle1:CAS:528:DyaK1MXltVOks7s%3D Occurrence Handle10.1016/S0038-0717(99)00063-2
H Høgh-Jensen J K Schjoerring (1994) ArticleTitleMeasurement of biological dinitrogen fixation in grassland – comparison of the enriched N-15 dilution and the natural N-15 abundance methods at different nitrogen application rates and defoliation frequencies Plant Soil 166 153–163 Occurrence Handle10.1007/BF00008328
H Høgh-Jensen J K Schjoerring (2001) ArticleTitleRhizodeposition of nitrogen by red clover, white clove and ryegrass leys Soil Biol. Biochem. 33 439–448 Occurrence Handle10.1016/S0038-0717(00)00183-8
H H Janzen S M McGinn (1991) ArticleTitleVolatile loss of nitrogen during decomposition of legume green manure Soil Biol. Biochem. 23 291–297 Occurrence Handle1:CAS:528:DyaK3MXisVKgsb8%3D Occurrence Handle10.1016/0038-0717(91)90066-S
F V Jørgensen ES Jensen J K Schjoerring (1999) ArticleTitleDinitrogen fixation in white clover grown in pure stand and mixture with ryegrass estimated by the immobilized N-15 isotope dilution method Plant Soil 208 293–305 Occurrence Handle10.1023/A:1004533430467
Y Kim N Tanaka (2002) ArticleTitleWinter N2O emission rate and its production rate in soil underlying the snowpack in a subboreal region, Japan J. Geophys. Res.-Atmosp. 107 D19
A Korsaeth T M Henriksen L R Bakken (2002) ArticleTitleTemporal changes in mineralization and immobilization of N during degradation of plant material: Implications for the plant N supply and nitrogen losses Soil Biol. Biochem. 34 789–799 Occurrence Handle1:CAS:528:DC%2BD38XjvVars74%3D Occurrence Handle10.1016/S0038-0717(02)00008-1
L Larsson M Ferm Å Kasimir-Klemedtsson L Klemedtsson (1998) ArticleTitleAmmonia and nitrous oxide emissions from grass and alfalfa mulches Nutr. Cycling Agroecosyst. 51 41–46 Occurrence Handle1:CAS:528:DyaK1cXis1akurg%3D Occurrence Handle10.1023/A:1009799126377
A R Lawson K B Kelly P W G Sale (2000) ArticleTitleDefoliation frequency and cultivar effects on the storage and utilisation of stolon and root reserves in white clover Aust. J. Agric. Res. 51 1039–1046 Occurrence Handle1:CAS:528:DC%2BD3cXosFyns7Y%3D Occurrence Handle10.1071/AR00039
S F Ledgard (2001) ArticleTitleNitrogen cycling in low input legume-based agriculture, with emphasis on legume/grass pastures Plant Soil 228 43–59 Occurrence Handle1:CAS:528:DC%2BD3MXhtlWkurw%3D Occurrence Handle10.1023/A:1004810620983
A Lüscher B Stäheli R Braun J Nösberger (2001) ArticleTitleLeaf area, competition with grass, and clover cultivar: Key factors to successful overwintering and fast regrowth of white clover (Trifolium repens L.) in spring Ann. Bot. 88 725–735 Occurrence Handle10.1006/anbo.2001.1509
C A Marriott A Haystead (1990) ArticleTitleThe effect of defoliation on the nitrogen economy of white clover: Regrowth and the remobilization of plant organic nitrogen Ann. Bot. 66 465–474 Occurrence Handle1:CAS:528:DyaK3MXpvVGnuw%3D%3D
G J A Ryle C E Powell A J Gordon (1985) ArticleTitleDefoliation in white clover: Regrowth, photosynthesis and N2 fixation Ann. Bot. 56 9–18 Occurrence Handle1:CAS:528:DyaL2MXkvVGhu7w%3D
E A Stockdale N H Lampkin M Hovi R Keatinge E K M Lennartsson D Macdonald S Padel F H Tattersall M S Wolfe C A Watson (2001) ArticleTitleAgronomic and environmental implications of organic farming systems Adv. Agron. 70 261–327 Occurrence Handle10.1016/S0065-2113(01)70007-7
I Sturite T M Henriksen T A Breland (2005) ArticleTitleDistinguishing between metabolically active and inactive roots by combined staining with 2,3,5-triphenyltetrazolium chloride and image colour analysis Plant Soil 271 75–82 Occurrence Handle1:CAS:528:DC%2BD2MXks1Ojtrk%3D Occurrence Handle10.1007/s11104-004-2027-0
Sturite I, Henriksen T M and Breland T A Longevity of white clover (Trifolium repens L.) leaves, stolons and roots under northern temperate climatic conditions. Ann. Bot. (submitted a)
Sturite I, Henriksen T M and Breland T A Winter losses of nitrogen and phosphorus from Italian ryegrass, meadow fescue and white clover under northern temperate climatic conditions. Agric. Ecosyst. Environ. (submitted b)
M M Svenning K Røsnes O Junttila (1997) ArticleTitleFrost tolerance and biochemical changes during hardening and dehardening in contrasting white clover populations Physiol. Plant. 101 31–37 Occurrence Handle1:CAS:528:DyaK2sXmsFejsLY%3D Occurrence Handle10.1111/j.1399-3054.1997.tb01816.x
J Swinnen J A Veen ParticleVan R Merckx (1994) ArticleTitleLosses of C-14 From roots of pulse-labeled wheat and barley during washing from soil Plant Soil 166 93–99 Occurrence Handle1:CAS:528:DyaK2MXjsFKmu7s%3D Occurrence Handle10.1007/BF02185485
E Syvasalo K Regina M Pihlatie M Esala (2004) ArticleTitleEmissions of nitrous oxide from boreal agricultural clay and loamy sand soils Nutr. Cycling Agroecosyst. 69 155–165 Occurrence Handle10.1023/B:FRES.0000029675.24465.fc
R Teepe R Brumme F Beese (2000) ArticleTitleNitrous oxide emissions from frozen soils under agricultural, fallow and forest land Soil Biol. Biochem. 32 1807–1810 Occurrence Handle1:CAS:528:DC%2BD3cXntFSlsLo%3D Occurrence Handle10.1016/S0038-0717(00)00078-X
G Tierney T Fahey P Groffman J Hardy R Fitzhugh C Driscoll (2001) ArticleTitleSoil freezing alters root dynamics in a northern hardwood forest Biogeochemistry 56 175–190 Occurrence Handle1:CAS:528:DC%2BD38XhtFajtLo%3D Occurrence Handle10.1023/A:1013072519889
Tronsmo A M 1982 Effects of low temperature hardening on resistance to biotic and abiotic stress factors in grasses. PhD Thesis, Agriculture University of Norway, Norway, Ås, 71 pp
C P Vance G H Heichel D K Barnes J W Bryan L E Johnson (1979) ArticleTitleNitrogen fixation, nodule development, and vegetative regrowth of alfalfa (Medicago sativa L.) following harvest Plant Physiol. 64 1–8 Occurrence Handle16660893 Occurrence Handle1:CAS:528:DyaE1MXlt1SnsLo%3D Occurrence Handle10.1104/pp.64.1.1
F P Vinther E S Jensen (2000) ArticleTitleEstimating legume N2 fixation in grass-clover mixtures of a grazed organic cropping system using two 15 N methods Agric. Ecosyst. Environ. 78 139–147 Occurrence Handle1:CAS:528:DC%2BD3cXhtFaju7w%3D Occurrence Handle10.1016/S0167-8809(99)00124-3
J J Volenec A Ourry B C Joern (1996) ArticleTitleA role for nitrogen reserves in forage regrowth and stress tolerance Physiol. Plant 97 185–193 Occurrence Handle1:CAS:528:DyaK28XjtFOrurw%3D Occurrence Handle10.1111/j.1399-3054.1996.tb00496.x
M Wachendorf R P Collins A Elgersma M Fothergill B F Frankow-Lindberg A Ghesquiere A Guckert M P Guinchard A Helgadóttir A Lüscher T Nolan P Nykanen-Kurki J Nosberger G Parente S Puzio I Rhodes C Robin A Ryan B Staheli S Stoffel F Taube J Connolly (2001) ArticleTitleOverwintering and growing season dynamics of Trifolium repens L. in mixture with Lolium perenne L.: A model approach to plant-environment interactions Ann. Bot. 88 683–702 Occurrence Handle10.1006/anbo.2001.1496
Western Ag Innovations Inc. 2001 Plant Root Simulator (PRSTM) Operations Manual. 411 Downey Road, Suite 3, Canada, 56 pp
J Woledge V Tewson I A Davidson (1990) ArticleTitleGrowth of grass clover mixtures during winter Grass Forage Sci. 45 191–202 Occurrence Handle10.1111/j.1365-2494.1990.tb02199.x
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Sturite, I., Uleberg, M.V., Henriksen, T.M. et al. Accumulation and Loss of Nitrogen in White Clover (Trifolium repens L.) Plant Organs as Affected by Defoliation Regime on Two Sites in Norway. Plant Soil 282, 165–182 (2006). https://doi.org/10.1007/s11104-005-5697-3
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DOI: https://doi.org/10.1007/s11104-005-5697-3