Skip to main content
SpringerLink
Log in

Iron deficiency-induced changes in carbon fixation and leaf elemental composition of sugar beet (Beta vulgaris) plants

  • Published:
Plant and Soil Aims and scope Submit manuscript

Abstract

In this experiment we (i) tested the hypothesis that, besides decreasing leaf C fixation, lime induced iron (Fe) deficiency increases root C fixation via PEP carboxylase and (ii) assessed the Fe-induced modifications in the elemental composition of plant tissues. Sugar beet plants were grown in nutrient solutions with Fe (45 μM Fe-EDTA; +Fe control) or in a similar nutrient solution without Fe (−Fe) and in presence of CaCO3 (1.0 gL−1), either labelled with 13C (20 at. %) or unlabelled. After 7 and 17 days from treatment imposition, plants were harvested and single organs analysed for total O, C, H, macro and micronutrients. 13C abundance was also assessed in control, unlabelled and labelled −Fe plants. Iron deficiency caused significant growth reductions; chlorophyll and net photosynthesis decreased markedly in Fe-deficient plants when compared to the controls, whereas leaf transpiration rates and stomatal conductance were not affected by Fe deficiency. Iron deficient plants had leaf biomass with lower C (2 to 4%) and higher O (3 to 5%) concentrations than +Fe plants. The δ13C was higher (less negative) in +Fe than in −Fe unlabelled plants. Iron deficient plants grown in the nutrient solution enriched with labelled CaCO3 absorbed a relatively small amount of labelled C, which was mainly recovered in the fine roots and accounted for less than 2% of total C gain in the 10 d treatment period. Evidences suggest that iron deficient sugar beets grown in the presence of CaCO3 do not markedly shift their C fixation from leaf RuBP to root PEPC.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • A.O.A.C. 1990. Official methods of analysis of the association of official analytical chemists. Washington, DC.

  • J Abadía A F López-Millán A D Rombolà A Abadía (2002) ArticleTitleOrganic acids and Fe deficiency: a review Plant Soil 241 IssueID1 75–86 Occurrence Handle10.1023/A:1016093317898

    Article  Google Scholar 

  • J Abadía J N Nishio E Monge L Montañés L Heras (1985) ArticleTitleMineral composition of peach tree leaves affected by iron chlorosis J. Plant Nutr 8 697–708

    Google Scholar 

  • S Andaluz A F López-Millán M L Peleato J Abadía A Abadía (2002) ArticleTitleIncreases in phosphoenolpyruvate carboxylase activity in iron-deficient sugar beet roots: analysis of spatial localization and post-transactional modification Plant Soil 241 IssueID1 43–48 Occurrence Handle10.1023/A:1016000216252 Occurrence Handle1:CAS:528:DC%2BD38XkvVajurc%3D

    Article  CAS  Google Scholar 

  • J Bialczyck Z Lechowski (1995) ArticleTitleChemical composition of xylem sap of tomato grown on bicarbonate containing medium J. Plant Nutr 18 2005–2021

    Google Scholar 

  • D D Davies (1973) ArticleTitleControl of and by pH Symp. Soc. Exp. Biol 27 513–529 Occurrence Handle1:STN:280:CSuC38bks1E%3D Occurrence Handle4361941

    CAS  PubMed  Google Scholar 

  • B Gruber H Kosegarten (2002) ArticleTitleDepressed growth of non-chlorotic vine grown in calcareous soil is an iron deficiency symptom prior to leaf chlorosis J. Plant Nutr. Soil Sci 165 111–117 Occurrence Handle10.1002/1522-2624(200202)165:1<111::AID-JPLN111>3.0.CO;2-B Occurrence Handle1:CAS:528:DC%2BD38XitVOgtLk%3D

    Article  CAS  Google Scholar 

  • Lajtha K and Marshall J D 1994. Sources of variation in the stable isotope composition of plants. In Stable isotopes in ecology and environmental sciences. Lajtha K. and R. H. Michener (Eds.) pp. 1–21. Blackwell Scientific Publications Oxford.

  • Lajtha K and Michener R H 1994 Introduction. In Stable isotopes in ecology and environmental sciences. Lajtha K. and R. H. Michener (Eds.) pp. VI–XIV. Blackwell Scientific Publications Oxford.

  • C Lance P Rustin (1984) ArticleTitleThe central role of malate in plant metabolism Physiol. Vég 22 625–641 Occurrence Handle1:CAS:528:DyaL2MXhvVagtL8%3D

    CAS  Google Scholar 

  • A Larbi A Abadía F Morales J Abadía (2004) ArticleTitleFe resupply to Fe-deficient sugar beet plants leads to rapid changes in the violaxanthin cycle and other photosynthetic characteristics without significant de novo chlorophyll synthesis Photosynth. Res 79 IssueID1 59–69 Occurrence Handle10.1023/B:PRES.0000011919.35309.5e Occurrence Handle1:CAS:528:DC%2BD2cXjtVGgsg%3D%3D

    Article  CAS  Google Scholar 

  • W L Lindsay A P Schwab (1982) ArticleTitleThe chemistry of iron in soils and its availability to plants J. Plant Nutr 5 821–840 Occurrence Handle1:CAS:528:DyaL38Xlt1Khsro%3D

    CAS  Google Scholar 

  • F López-Millán A F Morales A Abadía J Abadía (2000) ArticleTitleEffects of iron deficiency on the composition of the leaf apoplastic fluid and xylem sap in sugar beet. Implications for iron and carbon transport Plant Physiol 124 873–884 Occurrence Handle10.1104/pp.124.2.873 Occurrence Handle11027735

    Article  PubMed  Google Scholar 

  • F López-Millán A F Morales A Abadía J Abadía (2001) ArticleTitleChanges induced by Fe deficiency and Fe resupply in the organic acid metabolism of sugar beet (Beta vulgaris L.) leaves Physiol. Plant 112 31–38 Occurrence Handle11319012

    PubMed  Google Scholar 

  • A F López-Millán F Morales S Andaluz Y Gogorcena A Abadía J Rivas Particlede las J Abadía (2000) ArticleTitleResponses of sugar beet roots to iron deficiency. Changes in carbon assimilation and oxygen use Plant Physiol 124 885–897 Occurrence Handle11027736

    PubMed  Google Scholar 

  • K Mengel (1994) ArticleTitleIron availability in plant tissues – iron chlorosis on calcareous soils Plant Soil 165 275–283 Occurrence Handle1:CAS:528:DyaK2MXjtF2isbc%3D

    CAS  Google Scholar 

  • A D Rombolà W Brüggemann A F López-Millán J Abadía M Tagliavini B Marangoni P R Moog (2002) ArticleTitleBiochemical mechanisms of tolerance to Fe-deficiency in kiwifruit (A. deliciosa) Tree Physiol 22 IssueID12 869–875 Occurrence Handle12184976

    PubMed  Google Scholar 

  • V Römheld H Marschner (1986) ArticleTitleMobilization of iron in the rhizosphere of different plant species Adv. Plant Nutr 2 155–204

    Google Scholar 

  • N Terry (1979) The use of mineral nutrient stress in the study of limiting factors in photosynthesis R Marcelle H Clijsters M Poucke ParticleVan (Eds) Photosynthesis and plant development Dr. W. Junk Publishers The Hague 151–160

    Google Scholar 

  • N Terry (1980) ArticleTitleLimiting factors in photosynthesis. I. Use of iron stress to control photochemical capacity in vivo Plant Physiol 65 114–120 Occurrence Handle1:CAS:528:DyaL3cXhtVOmtrk%3D

    CAS  Google Scholar 

  • E M Vapaavuori P Pelkonen (1985) ArticleTitleHCO −3 uptake through the roots and its effect on the productivity of willow cuttings Plant Cell Environ 8 531–534

    Google Scholar 

  • T Young N Terry (1982) ArticleTitleTransport of iron into leaves following iron resupply to iron-stressed sugar beet plants J. Plant Nutr 5 1273–1283 Occurrence Handle1:CAS:528:DyaL3sXlt1Grug%3D%3D

    CAS  Google Scholar 

  • T Zaharieva Y Gogorcena J Abadía (2004) ArticleTitleDynamics of metabolic responses in sugar beet roots to iron deficiency Plant Science 166 1045–1050 Occurrence Handle1:CAS:528:DC%2BD2cXhs12mtrg%3D

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dipartimento di Colture Arboree, Università di Bologna, Via G. Fanin 46, 40127, Bologna, Italy

    A. D. Rombolà, E. Baldi, B. Marangoni & M. Tagliavini

  2. Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas (EEAD-CSIC), Apdo. 202, E-50080, Zaragoza, Spain

    Y. Gogorcena, A. Larbi, F. Morales & J. Abadía

Authors
  1. A. D. Rombolà
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Gogorcena
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Larbi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. F. Morales
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. Baldi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. B. Marangoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. Tagliavini
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. J. Abadía
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. D. Rombolà.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rombolà, A.D., Gogorcena, Y., Larbi, A. et al. Iron deficiency-induced changes in carbon fixation and leaf elemental composition of sugar beet (Beta vulgaris) plants. Plant Soil 271, 39–45 (2005). https://doi.org/10.1007/s11104-004-2001-x

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11104-004-2001-x

Keywords

Search

Navigation