Abstract
Key message
A sugarcane MYB present in the culm induces suberin biosynthesis and is involved both with fatty acid and phenolics metabolism.
Abstract
Few transcription factors have been described as regulators of cell wall polymers deposition in C4 grasses. Particularly, regulation of suberin biosynthesis in this group of plants remains poorly understood. Here, we showed that the sugarcane MYB transcription factor ShMYB78 is an activator of suberin biosynthesis and deposition. ShMYB78 was identified upon screening genes whose expression was upregulated in sugarcane internodes undergoing suberization during culm development or triggered by wounding. Agrobacterium-mediated transient expression of ShMYB78 in Nicotiana benthamiana leaves induced the ectopic deposition of suberin and its aliphatic and aromatic monomers. Further, the expression of suberin-related genes was induced by ShMYB78 heterologous expression in Nicotiana benthamiana leaves. ShMYB78 was shown to be a nuclear protein based on its presence in sugarcane internode nuclear protein extracts, and protoplast transactivation assays demonstrated that ShMYB78 activates the promoters of the sugarcane suberin biosynthetic genes β-ketoacyl-CoA synthase (ShKCS20) and caffeic acid-O-methyltransferase (ShCOMT). Our results suggest that ShMYB78 may be involved in the transcriptional regulation of suberin deposition, from fatty acid metabolism to phenylpropanoid biosynthesis, in sugarcane internodes.
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All data generated or analyzed during this study are included in this published article [and its supplementary information files].
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Acknowledgements
We thank Dr Marie-Anne van Sluys, coordinator of the GaTElab at the Institute of Biosciences, University of São Paulo, Brazil, for the access to R570 BAC library, and specially to Tatiana Silveira Corrêa and Geovani Ragagnin for support in the library screening. We thank the access to Confocal Zeiss LSM780-NLO and assistance provided by the National Institute of Science and Technology on Photonics Applied to Cell Biology (INFABIC) at the State University of Campinas, Brazil; INFABIC is co-funded by São Paulo Research Foundation (FAPESP_08/57906-3) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq_573913/2008-0). We thank the access to microscope Olympus BX51 to Dr Juliana Meyer at the State University of Campinas, Brazil. RF thanks FAPESP and CNPq for postdoctoral fellowships (FAPESP Grant 2015/05437-3; CNPq grant 104051/2018-3) and PM thanks CNPq for a research fellowship. IC thanks FAPESP for the BIOEN Young Investigators Awards research grant (Processo FAPESP n° 2015/02527-1) and CNPq for the research fellowship 302927/2018-2.
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RF was granted FAPESP and CNPq postdoctoral fellowships (FAPESP Grant 2015/05437-3; CNPq Grant 104051/2018-3) and PM received a CNPq research fellowship. IC thanks FAPESP for the BIOEN Young Investigators Awards research Grant (Processo FAPESP n° 2015/02527-1) and CNPq for the research fellowship 302927/2018-2.
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RF and PM designed the research; RF, JPL, SSF and BC performed the research; EK, SS, MSB and LS provided technical support; RF, EK, SSF, IC and PM analyzed and interpreted the data; RF, SSF, IC and PM wrote the manuscript.
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Figueiredo, R., Portilla Llerena, J.P., Kiyota, E. et al. The sugarcane ShMYB78 transcription factor activates suberin biosynthesis in Nicotiana benthamiana. Plant Mol Biol 104, 411–427 (2020). https://doi.org/10.1007/s11103-020-01048-1
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DOI: https://doi.org/10.1007/s11103-020-01048-1