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WSL3, a component of the plastid-encoded plastid RNA polymerase, is essential for early chloroplast development in rice

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Abstract

Plastid-encoded plastid RNA polymerase (PEP), a dominant RNA polymerase in mature chloroplasts, consists of core subunits and peripheral subunits. Despite the importance of the peripheral subunits in control of PEP activity it is unclear how they interact with one another to exert physiological effects on chloroplast development and plant growth, especially in rice. Here, we report a mutant, designated wsl3 that lacks a peripheral subunit in rice. We isolated the WSL3 gene encoding an essential peripheral subunit of rice PEP complex, OsPAP1/OspTAC3 by map-based cloning, and verified its function by complementation analysis. The wsl3 mutant showed a typical expression pattern of plastid-encoded genes, suggesting that PEP activity was impaired. Using immunofluorescent labeling and immunoblotting, we found that WSL3 was localized to the chloroplast and associated with the nucleoid. In addition, we demonstrated that WSL3 interacted with PEP subunits in Y2H, BiFC and pull-down experiments. Furthermore, a cpDNA IP assay revealed that WSL3 was associated with the PEP complex during the entire transcription process. We provide evidence suggesting that WSL3 is essential for early chloroplast development by interacting with subunits of the PEP complex.

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Acknowledgments

This research was supported by Grants from the 863 Program (2014AA10A603-15), National Science and Technology Support Program (2013BAD01B02-16), a project from the Ministry of Agriculture of China for Transgenic Research (2014ZX08001-004), Jiangsu Science and Technology Development Program (BE2013301), Key Laboratory of Biology, Genetics and Breeding of Japonica Rice in Mid-lower Yangtze River, Ministry of Agriculture, P. R. China, and Jiangsu Collaborative Innovation Center for Modern Crop Production.

Author contributions

Jianmin Wan supervised the project. Liwei Wang performed almost all the experiments. Liwei Wang wrote the paper and Chunming Wang revised the paper. Yihua Wang identified the wsl3 mutant. Liwei Wang, Yihua Wang and Mei Niu performed genetic analysis and mapping of WSL3. Liwei Wang, Yulong Ren, Kunneng Zhou and Huan Zhang performed subcellular localization of WSL3 protein. Liwei Wang, Yulong Ren, Qibing Lin and Fuqing Wu performed some of the BiFC assay, and Pull-Down assay. Xin Zhang, Xiuping Guo, Ling Jiang and Shanshan Zhu generated the transgenic plants. Zhijun Cheng, Jiulin Wang, Cailin Wang, Jie Wang and Zhichao Zhao cultivated the transgenic plants in the field.

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    Authors and Affiliations

    1. State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

      Liwei Wang, Chunming Wang, Yihua Wang, Mei Niu, Kunneng Zhou, Huan Zhang, Ling Jiang & Jianmin Wan

    2. National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

      Yulong Ren, Qibing Lin, Fuqing Wu, Zhijun Cheng, Jiulin Wang, Xin Zhang, Xiuping Guo, Cailin Lei, Jie Wang, Shanshan Zhu, Zhichao Zhao & Jianmin Wan

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    1. Liwei Wang
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    Correspondence to Jianmin Wan.

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    Liwei Wang and Chunming Wang have contributed equally to this work.

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    Wang, L., Wang, C., Wang, Y. et al. WSL3, a component of the plastid-encoded plastid RNA polymerase, is essential for early chloroplast development in rice. Plant Mol Biol 92, 581–595 (2016). https://doi.org/10.1007/s11103-016-0533-0

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