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A single-repeat MYB transcription repressor, MYBH, participates in regulation of leaf senescence in Arabidopsis

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Abstract

Leaf senescence, the final stage of leaf development, is regulated tightly by endogenous and environmental signals. MYBS3, a MYB transcription factor with a single DNA-binding domain, mediates sugar signaling in rice. Here we report that an Arabidopsis MYBS3 homolog, MYBH, plays a critical role in developmentally regulated and dark-induced leaf senescence by repressing transcription. Expression of MYBH was enhanced in older and dark-treated leaves. Gain- and loss-of-function analysis indicated that MYBH was involved in the onset of leaf senescence. Plants constitutively overexpressing MYBH underwent premature leaf senescence and showed enhanced expression of leaf senescence marker genes. In contrast, the MYBH mutant line, mybh-1, exhibited a delayed-senescence phenotype. The EAR repression domain was required for MYBH-regulated leaf senescence. Overexpression and knockout of MYBH repressed and enhanced auxin-responsive gene expression, respectively. MYBH repressed the auxin-amido synthase genes DFL1/GH3.6 and DFL2/GH3.10, which regulate auxin homoeostasis, by binding directly to the TA box in each of their regulatory regions. An auxin-responsive phenotype was enhanced in MYBH overexpression lines and reduced in mybh knockout lines. Overexpression of MYBH enhanced gene expression of SAUR36, an auxin-promoted leaf senescence key regulator, and accelerated ABA- and ethylene-induced leaf senescence in transgenic Arabidopsis plants. Our results suggest that the role of MYBH in controlling auxin homeostasis accounts for its capacity to participate in regulation of age- and darkness-induced leaf senescence in Arabidopsis.

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References

  • Balazadeh S, Siddiqui H, Allu AD, Matallana-Ramirez LP, Caldana C, Mehrnia M, Zanor MI, Kohler B, Mueller-Roeber B (2010) A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence. Plant J 62:250–264

    Article  CAS  PubMed  Google Scholar 

  • Becker W, Apel K (1993) Differences in gene-expression between natural and artificially induced leaf senescence. Planta 189:74–79

    Article  CAS  Google Scholar 

  • Besseau S, Li J, Palva ET (2012) WRKY54 and WRKY70 co-operate as negative regulators of leaf senescence in Arabidopsis thaliana. J Exp Bot 63:2667–2679

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Brand L, Horler M, Nuesch E, Vassalli S, Barrell P, Yang W, Jefferson RA, Grossniklaus U, Curtis MD (2006) A versatile and reliable two-component system for tissue-specific gene induction in Arabidopsis. Plant Physiol 141:1194–1204

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Brouwer B, Ziolkowska A, Bagard M, Keech O, Gardestrom P (2012) The impact of light intensity on shade-induced leaf senescence. Plant, Cell Environ 35:1084–1098

    Article  Google Scholar 

  • Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ (2005) Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J 42:567–585

    Article  CAS  PubMed  Google Scholar 

  • Chen GH, Chan YL, Liu CP, Wang LC (2012) Ethylene response pathway is essential for ARABIDOPSIS A-FIFTEEN function in floral induction and leaf senescence. Plant Signal Behav 7:457–460

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743

    Article  CAS  PubMed  Google Scholar 

  • Curtis MD, Grossniklaus U (2003) A gateway cloning vector set for high-throughput functional analysis of genes in planta. Plant Physiol 133:462–469

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Dong J, Chen C, Chen Z (2003) Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response. Plant Mol Biol 51:21–37

    Article  CAS  PubMed  Google Scholar 

  • Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L (2010) MYB transcription factors in Arabidopsis. Trends Plant Sci 15:573–581

    Article  CAS  PubMed  Google Scholar 

  • Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW (2005) AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana. Development 132:4563–4574

    Article  CAS  PubMed  Google Scholar 

  • Fan M, Xu C, Xu K, Hu Y (2012) LATERAL ORGAN BOUNDARIES DOMAIN transcription factors direct callus formation in Arabidopsis regeneration. Cell Res 22:1169–1180

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Gepstein S, Sabehi G, Carp MJ, Hajouj T, Nesher MF, Yariv I, Dor C, Bassani M (2003) Large-scale identification of leaf senescence-associated genes. Plant J 36:629–642

    Article  CAS  PubMed  Google Scholar 

  • Gombert J, Etienne P, Ourry A, Le Dily F (2006) The expression patterns of SAG12/Cab genes reveal the spatial and temporal progression of leaf senescence in Brassica napus L. with sensitivity to the environment. J Exp Bot 57:1949–1956

    Article  CAS  PubMed  Google Scholar 

  • Grossmann K (2010) Auxin herbicides: current status of mechanism and mode of action. Pest Manag Sci 66:113–120

    CAS  PubMed  Google Scholar 

  • Guo Y, Gan S (2006) AtNAP, a NAC family transcription factor, has an important role in leaf senescence. Plant J 46:601–612

    Article  CAS  PubMed  Google Scholar 

  • Guo Y, Gan S (2011) AtMYB2 regulates whole plant senescence by inhibiting cytokinin-mediated branching at late stages of development in Arabidopsis. Plant Physiol 156:1612–1619

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Guo Y, Cai Z, Gan S (2004) Transcriptome of Arabidopsis leaf senescence. Plant, Cell Environ 27:521–549

    Article  CAS  Google Scholar 

  • Hansen H, Grossmann K (2000) Auxin-induced ethylene triggers abscisic acid biosynthesis and growth inhibition. Plant Physiol 124:1437–1448

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Hickman R, Hill C, Penfold CA, Breeze E, Bowden L, Moore JD, Zhang P, Jackson A, Cooke E, Bewicke-Copley F, Mead A, Beynon J, Wild DL, Denby KJ, Ott S, Buchanan-Wollaston V (2013) A local regulatory network around three NAC transcription factors in stress responses and senescence in Arabidopsis leaves. Plant J 75:26–39

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Hiratsu K, Matsui K, Koyama T, Ohme-Takagi M (2003) Dominant repression of target genes by chimeric repressors that include the EAR motif, a repression domain, in Arabidopsis. Plant J 34:733–739

    Article  CAS  PubMed  Google Scholar 

  • Hou K, Wu W, Gan S (2013) SAUR36, a SMALL AUXIN UP RNA gene, is involved in the promotion of leaf senescence in Arabidopsis. Plant Physiol 161:1002–1009

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Huang CK, Huang LF, Huang JJ, Wu SJ, Yeh CH, Lu CA (2010) A DEAD-box protein, AtRH36, is essential for female gametophyte development and is involved in rRNA biogenesis in Arabidopsis. Plant Cell Physiol 51:694–706

    Article  Google Scholar 

  • Ikeda M, Ohme-Takagi M (2009) A novel group of transcriptional repressors in Arabidopsis. Plant Cell Physiol 50:970–975

    Article  CAS  PubMed  Google Scholar 

  • Jan A, Maruyama K, Todaka D, Kidokoro S, Abo M, Yoshimura E, Shinozaki K, Nakashima K, Yamaguchi-Shinozaki K (2013) OsTZF1, a CCCH-tandem zinc finger protein, confers delayed senescence and stress tolerance in rice by regulating stress-related genes. Plant Physiol 161:1202–1216

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Jaradat MR, Feurtado JA, Huang D, Lu Y, Cutler AJ (2013) Multiple roles of the transcription factor AtMYBR1/AtMYB44 in ABA signaling, stress responses, and leaf senescence. BMC Plant Biol 13:192

    Article  PubMed Central  PubMed  Google Scholar 

  • Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907

    PubMed Central  CAS  PubMed  Google Scholar 

  • Jibran R, Hunter DA, Dijkwel PP (2013) Hormonal regulation of leaf senescence through integration of developmental and stress signals. Plant Mol Biol 82:547–561

    Article  CAS  PubMed  Google Scholar 

  • Kandasamy MK, Gilliland LU, McKinney EC, Meagher RB (2001) One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell 13:1541–1554

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kaufmann K, Muino JM, Osteras M, Farinelli L, Krajewski P, Angenent GC (2010) Chromatin immunoprecipitation (ChIP) of plant transcription factors followed by sequencing (ChIP-SEQ) or hybridization to whole genome arrays (ChIP-CHIP). Nat Protoc 5:457–472

    Article  CAS  PubMed  Google Scholar 

  • Kim JI, Murphy AS, Baek D, Lee SW, Yun DJ, Bressan RA, Narasimhan ML (2011) YUCCA6 over-expression demonstrates auxin function in delaying leaf senescence in Arabidopsis thaliana. J Exp Bot 62:3981–3992

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kim YS, Sakuraba Y, Han SH, Yoo SC, Paek NC (2013) Mutation of the Arabidopsis NAC016 transcription factor delays leaf senescence. Plant Cell Physiol 54:1660–1672

    Article  CAS  PubMed  Google Scholar 

  • Kobayashi K, Baba S, Obayashi T, Sato M, Toyooka K, Keranen M, Aro EM, Fukaki H, Ohta H, Sugimoto K, Masuda T (2012) Regulation of root greening by light and auxin/cytokinin signaling in Arabidopsis. Plant Cell 24:1081–1095

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kong Z, Li M, Yang W, Xu W, Xue Y (2006) A novel nuclear-localized CCCH-type zinc finger protein, OsDOS, is involved in delaying leaf senescence in rice. Plant Physiol 141:1376–1388

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Koyama T, Nii H, Mitsuda N, Ohta M, Kitajima S, Ohme-Takagi M, Sato F (2013) A regulatory cascade involving class II ETHYLENE RESPONSE FACTOR transcriptional repressors operates in the progression of leaf senescence. Plant Physiol 162:991–1005

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kraft M, Kuglitsch R, Kwiatkowski J, Frank M, Grossmann K (2007) Indole-3-acetic acid and auxin herbicides up-regulate 9-cis-epoxycarotenoid dioxygenase gene expression and abscisic acid accumulation in cleavers (Galium aparine): interaction with ethylene. J Exp Bot 58:1497–1503

    Article  CAS  PubMed  Google Scholar 

  • Kwon Y, Kim JH, Nguyen HN, Jikumaru Y, Kamiya Y, Hong SW, Lee H (2013) A novel Arabidopsis MYB-like transcription factor, MYBH, regulates hypocotyl elongation by enhancing auxin accumulation. J Exp Bot 64:3911–3922

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Lázaro A, Gómez-Zambrano A, López-González L, Piñeiro M, Jarillo JA (2008) Mutations in the Arabidopsis SWC6 gene, encoding a component of the SWR1 chromatin remodelling complex, accelerate flowering time and alter leaf and flower development. J Exp Bot 59:653–666

    Article  PubMed  Google Scholar 

  • Lee S, Seo PJ, Lee HJ, Park CM (2012) A NAC transcription factor NTL4 promotes reactive oxygen species production during drought-induced leaf senescence in Arabidopsis. Plant J 70:831–844

    Article  CAS  PubMed  Google Scholar 

  • Lichtenthaler HK, Buschmann C, Rinderle U, Schmuck G (1986) Application of chlorophyll fluorescence in ecophysiology. Radiat Environ Biophys 25:297–308

    Article  CAS  PubMed  Google Scholar 

  • Lim PO, Kim HJ, Nam HG (2007) Leaf senescence. Annu Rev Plant Biol 58:115–136

    Article  CAS  PubMed  Google Scholar 

  • Lin JF, Wu SH (2004) Molecular events in senescing Arabidopsis leaves. Plant J 39:612–628

    Article  CAS  PubMed  Google Scholar 

  • Lu CA, Ho TH, Ho SL, Yu SM (2002) Three novel MYB proteins with one DNA binding repeat mediate sugar and hormone regulation of alpha-amylase gene expression. Plant Cell 14:1963–1980

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Lu CA, Lin CC, Lee KW, Chen JL, Huang LF, Ho SL, Liu HJ, Hsing YI, Yu SM (2007) The SnRK1A protein kinase plays a key role in sugar signaling during germination and seedling growth of rice. Plant Cell 19:2484–2499

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Miao Y, Laun T, Zimmermann P, Zentgraf U (2004) Targets of the WRKY53 transcription factor and its role during leaf senescence in Arabidopsis. Plant Mol Biol 55:853–867

    Article  CAS  PubMed  Google Scholar 

  • Moore B, Zhou L, Rolland F, Hall Q, Cheng WH, Liu YX, Hwang I, Jones T, Sheen J (2003) Role of the Arabidopsis glucose sensor HXK1 in nutrient, light, and hormonal signaling. Science 300:332–336

    Article  CAS  PubMed  Google Scholar 

  • Morris K, MacKerness SA, Page T, John CF, Murphy AM, Carr JP, Buchanan-Wollaston V (2000) Salicylic acid has a role in regulating gene expression during leaf senescence. Plant J 23:677–685

    Article  CAS  PubMed  Google Scholar 

  • Nakazawa M, Yabe N, Ichikawa T, Yamamoto YY, Yoshizumi T, Hasunuma K, Matsui M (2001) DFL1, an auxin-responsive GH3 gene homologue, negatively regulates shoot cell elongation and lateral root formation, and positively regulates the light response of hypocotyl length. Plant J 25:213–221

    Article  CAS  PubMed  Google Scholar 

  • Polanska L, Vicankova A, Novakova M, Malbeck J, Dobrev PI, Brzobohaty B, Vankova R, Machackova I (2007) Altered cytokinin metabolism affects cytokinin, auxin, and abscisic acid contents in leaves and chloroplasts, and chloroplast ultrastructure in transgenic tobacco. J Exp Bot 58:637–649

    Article  CAS  PubMed  Google Scholar 

  • Quirino BF, Normanly J, Amasino RM (1999) Diverse range of gene activity during Arabidopsis thaliana leaf senescence includes pathogen-independent induction of defense-related genes. Plant Mol Biol 40:267–278

    Article  CAS  PubMed  Google Scholar 

  • Robatzek S, Somssich IE (2001) A new member of the Arabidopsis WRKY transcription factor family, AtWRKY6, is associated with both senescence- and defence-related processes. Plant J 28:123–133

    Article  CAS  PubMed  Google Scholar 

  • Rubio-Somoza I, Martinez M, Abraham Z, Diaz I, Carbonero P (2006) Ternary complex formation between HvMYBS3 and other factors involved in transcriptional control in barley seeds. Plant J 47:269–281

    Article  CAS  PubMed  Google Scholar 

  • Seo PJ, Park CM (2011) Signaling linkage between environmental stress resistance and leaf senescence in Arabidopsis. Plant Signal Behav 6:1564–1566

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Shahnejat-Bushehri S, Mueller-Roeber B, Balazadeh S (2012) Arabidopsis NAC transcription factor JUNGBRUNNEN1 affects thermomemory-associated genes and enhances heat stress tolerance in primed and unprimed conditions. Plant Signal Behav 7:1518–1521

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Sterling T, Hal J (1997) Mechanism of action of natural auxins and the auxinic herbicides. Rev Toxicol 1:111–142

    CAS  Google Scholar 

  • Su CF, Wang YC, Hsieh TH, Lu CA, Tseng TH, Yu SM (2010) A novel MYBS3-dependent pathway confers cold tolerance in rice. Plant Physiol 153:145–158

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Takase T, Nakazawa M, Ishikawa A, Manabe K, Matsui M (2003) DFL2, a new member of the Arabidopsis GH3 gene family, is involved in red light-specific hypocotyl elongation. Plant Cell Physiol 44:1071–1080

    Article  CAS  PubMed  Google Scholar 

  • Upadhyay RK, Gupta A, Ranjan S, Singh R, Pathre UV, Nath P, Sane AP (2014) The EAR motif controls the early flowering and senescence phenotype mediated by over-expression of SlERF36 and is partly responsible for changes in stomatal density and photosynthesis. PLoS ONE 18:e101995

    Article  Google Scholar 

  • Vainonen JP, Jaspers P, Wrzaczek M, Lamminmaki A, Reddy RA, Vaahtera L, Brosche M, Kangasjarvi J (2012) RCD1-DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana. Biochem J 442:573–581

    Article  CAS  PubMed  Google Scholar 

  • van der Graaff E, Schwacke R, Schneider A, Desimone M, Flugge UI, Kunze R (2006) Transcription analysis of arabidopsis membrane transporters and hormone pathways during developmental and induced leaf senescence. Plant Physiol 141:776–792

    Article  PubMed Central  PubMed  Google Scholar 

  • Volfová A, Chvojka L, Friedrich A (1978) The effect of kinetin and auxin on the chloroplast structure and chlorophyll content in wheat coleoptiles. Biol Plant 20:440–445

    Article  Google Scholar 

  • Weaver LM, Gan S, Quirino B, Amasino RM (1998) A comparison of the expression patterns of several senescence-associated in response to stress and hormone treatment. Plant Mol Biol 37:455–469

    Article  CAS  PubMed  Google Scholar 

  • Woo HR, Kim JH, Nam HG, Lim PO (2004) The delayed leaf senescence mutants of Arabidopsis, ore1, ore3, and ore9 are tolerant to oxidative stress. Plant Cell Physiol 45:923–932

    Article  CAS  PubMed  Google Scholar 

  • Woo HR, Kim JH, Kim J, Kim J, Lee U, Song IJ, Kim JH, Lee HY, Nam HG, Lim PO (2010) The RAV1 transcription factor positively regulates leaf senescence in Arabidopsis. J Exp Bot 61:3947–3957

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Wu K, Zhang L, Zhou C, Yu CW, Chaikam V (2008) HDA6 is required for jasmonate response, senescence and flowering in Arabidopsis. J Exp Bot 59:225–234

    Article  CAS  PubMed  Google Scholar 

  • Wu FH, Shen SC, Lee LY, Lee SH, Chan MT, Lin CS (2009) Tape-Arabidopsis Sandwich—a simpler Arabidopsis protoplast isolation method. Plant Methods 5:16

    Article  PubMed Central  PubMed  Google Scholar 

  • Wu A, Allu AD, Garapati P, Siddiqui H, Dortay H, Zanor MI, Asensi-Fabado MA, Munne-Bosch S, Antonio C, Tohge T, Fernie AR, Kaufmann K, Xue GP, Mueller-Roeber B, Balazadeh S (2012) JUNGBRUNNEN1, a reactive oxygen species-responsive NAC transcription factor, regulates longevity in Arabidopsis. Plant Cell 24:482–506

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Xu F, Meng T, Li P, Yu Y, Cui Y, Wang Y, Gong Q, Wang NN (2011) A soybean dual-specificity kinase, GmSARK, and its Arabidopsis homolog, AtSARK, regulate leaf senescence through synergistic actions of auxin and ethylene. Plant Physiol 157:2131–2153

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Yang SD, Seo PJ, Yoon HK, Park CM (2011) The Arabidopsis NAC transcription factor VNI2 integrates abscisic acid signals into leaf senescence via the COR/RD genes. Plant Cell 23:2155–2168

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Zentgraf U, Laun T, Miao Y (2010) The complex regulation of WRKY53 during leaf senescence of Arabidopsis thaliana. Eur J Cell Biol 89:133–137

    Article  CAS  PubMed  Google Scholar 

  • Zhang X, Ju HW, Chung MS, Huang P, Ahn SJ, Kim CS (2011) The R-R-type MYB-like transcription factor, AtMYBL, is involved in promoting leaf senescence and modulates an abiotic stress response in Arabidopsis. Plant Cell Physiol 52:138–148

    Article  CAS  PubMed  Google Scholar 

  • Zhou X, Jiang Y, Yu D (2011) WRKY22 transcription factor mediates dark-induced leaf senescence in Arabidopsis. Mol Cells 31:303–313

    Article  PubMed Central  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by a grant (98-2311-B-008-002-MY3) from the National Science Council of the Republic of China.

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  1. Department of Life Science, National Central University, Jhongli City, 320, Taoyuan County, Taiwan, ROC

    Chun-Kai Huang, Pei-Ching Lo, Shaw-Jye Wu, Ching-Hui Yeh & Chung-An Lu

  2. Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Jhongli City, 320, Taoyuan County, Taiwan, ROC

    Li-Fen Huang

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Huang, CK., Lo, PC., Huang, LF. et al. A single-repeat MYB transcription repressor, MYBH, participates in regulation of leaf senescence in Arabidopsis. Plant Mol Biol 88, 269–286 (2015). https://doi.org/10.1007/s11103-015-0321-2

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