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Expression of Terpenoids 1, a glandular trichome-specific transcription factor from tomato that activates the terpene synthase 5 promoter

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Abstract

Terpene biosynthesis in tomato glandular trichomes has been well studied, with most if not all terpene synthases (TPSs) being identified. However, transcription factors (TFs) that regulate TPSs have not yet been discovered from tomato. In order to unravel the transcriptional regulation of the Solanum lycopersicum linalool synthase (SlMTS1, recently renamed SlTPS5) gene in glandular trichomes, we functionally dissected its promoter. A 207 bp fragment containing the minimal promoter and the 5′UTR appeared to be sufficient for trichome-specific expression in transgenic plants. Yeast-one-hybrid screens with this fragment identified a glandular trichome-specific transcription factor, designated Expression of Terpenoids 1 (SlEOT1). SlEOT1 is a member of a conserved family of TFs that includes the Arabidopsis Stylish 1 (AtSTY1) and Short Internode (AtSHI) genes. The EOT1 protein localized to the nucleus and specifically transactivated the SlTPS5 promoter in Nicotiana benthamiana leaves.

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Acknowledgments

We thank Mattijs Bliek (Vrije Universiteit, Amsterdam) for kindly providing the yeast strain PJ69-4A, Carlos Galvan-Ampudia, Ronald Breedijk and Maria Koini for help with confocal imaging constructs and microscopy, Kai Ament for constructing the trichome cDNA library, Juan M. Alba-Cano for help with the statistical analyses and Harold Lemeris, Ludeck Tikovsky and Thijs Hendrix for the excellent care of the tomato plants. This work was supported by KeyGene and the Technological Top Institute-Green Genetics Grant 1C002RP.

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  1. Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands

    Eleni A. Spyropoulou, Michel A. Haring & Robert C. Schuurink

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  1. Eleni A. Spyropoulou
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  2. Michel A. Haring
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  3. Robert C. Schuurink
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Correspondence to Robert C. Schuurink.

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Spyropoulou, E.A., Haring, M.A. & Schuurink, R.C. Expression of Terpenoids 1, a glandular trichome-specific transcription factor from tomato that activates the terpene synthase 5 promoter. Plant Mol Biol 84, 345–357 (2014). https://doi.org/10.1007/s11103-013-0142-0

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