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The Arabidopsis thaliana transcriptional activator STYLISH1 regulates genes affecting stamen development, cell expansion and timing of flowering

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Abstract

SHORT-INTERNODES/STYLISH (SHI/STY)-family proteins redundantly regulate development of lateral organs in Arabidopsis thaliana. We have previously shown that STY1 interacts with the promoter of the auxin biosynthesis gene YUCCA (YUC)4 and activates transcription of the genes YUC4, YUC8 and OCTADECANOID-RESPONSIVE ARABIDOPSIS AP2/ERF (ORA)59 independently of protein translation. STY1 also affects auxin levels and auxin biosynthesis rates. Here we show that STY1 induces the transcription of 16 additional genes independently of protein translation. Several of these genes are tightly co-expressed with SHI/STY-family genes and/or down-regulated in SHI/STY-family multiple mutant lines, suggesting them to be regulated by SHI/STY proteins during plant development. The majority of the identified genes encode transcription factors or cell expansion-related enzymes and functional studies suggest their involvement in stamen and leaf development or flowering time regulation.

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Acknowledgments

We thank Yunde Zhao, Cristina Ferrandiz, Johan Memelink and Elin Övernäs for providing seeds, and John Chandler for critical reading of the manuscript. This work was supported by grants from the Swedish Research Council Formas to Eva Sundberg, the Nilsson-Ehle-donations and the Royal Swedish Academy of Science to Veronika Ståldal.

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  1. Song Chen

    Present address: Cologne Biocenter, Botanical Institute, University of Cologne, Zülpicher Straße 47b, 50674, Köln, Germany

  2. D. Magnus Eklund

    Present address: School of Biological Sciences, Monash University, Clayton Campus, Melbourne, VIC, 3800, Australia

Authors and Affiliations

  1. Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Linnean Centre of Plant Biology in Uppsala, Swedish University of Agricultural Sciences, PO-Box 7080, 750 07, Uppsala, Sweden

    Veronika Ståldal, Izabela Cierlik, Song Chen, Katarina Landberg, Mattias Myrenås, Jens F. Sundström, D. Magnus Eklund & Eva Sundberg

  2. Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC, V5A 1S6, Canada

    Tammy Baylis

  3. Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 901 83, Umeå, Sweden

    Karin Ljung

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  1. Veronika Ståldal
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Correspondence to Eva Sundberg.

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Ståldal, V., Cierlik, I., Chen, S. et al. The Arabidopsis thaliana transcriptional activator STYLISH1 regulates genes affecting stamen development, cell expansion and timing of flowering. Plant Mol Biol 78, 545–559 (2012). https://doi.org/10.1007/s11103-012-9888-z

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