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Transcript profiling of oilseed rape (Brassica napus) primed for biocontrol differentiate genes involved in microbial interactions with beneficial Bacillus amyloliquefaciens from pathogenic Botrytis cinerea

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Abstract

Many microorganisms interact with plants but information is insufficient concerning requirements for plant colonization and if interactions become beneficial or detrimental. Pretreatment of oilseed rape (Brassica napus) with Bacillus results in disease suppression upon challenge with pathogens. We have studied transcriptome effects on oilseed rape primed with the Bacillus amyloliquefaciens 5113 biocontrol strain and compared that with effects of the fungal pathogen Botrytis cinerea. Using the cDNA-AFLP technique 21,700 transcript fragments were obtained of which 120 were differentially expressed and verified by northern blot analysis for selected transcripts. Priming with Bacillus caused greater effect on leaf than root transcripts where sequencing and BLAST analysis suggested many of the transcripts to be involved in metabolism and bioenergy. Bacillus and Botrytis treatment also changed metabolic gene expression in addition to signaling and transcription control genes as well as a potential disease resistance (TIR-NBS-LRR) gene. The pathogen provoked non-primed plant profile was less dominated by metabolism than Bacillus and Bacillus–Botrytis treated plants. Several transcripts were homologues to unknown genes in the different treatments. Altogether Bacillus treatment of roots cause a systemic gene expression in leaves suggested to result in a metabolic reprogramming as a major event during priming.

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Abbreviations

hpi:

Hours post inoculation

ISR:

Induced systemic resistance

JA:

Jasmonic acid

LPS:

Lipopolysaccharides

PGPR:

Plant Growth Promoting Rhizobacteria

SA:

Salicylic acid

SAR:

Systemic acquired resistance

TDF:

Transcript derived fragment

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Acknowledgements

This work was supported by grants from the IMOP programme SLU, Carl Tryggers fund, FORMAS, Nilsson-Ehle fund, Helge Ax:son Johnson fund, and Persson fund. We are grateful to Johanna Lagensjö and Namita Wadke for assistance in TDF cloning.

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  1. Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7080, 750 07, Uppsala, Sweden

    Bejai R. Sarosh, Jesper Danielsson & Johan Meijer

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  1. Bejai R. Sarosh
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  2. Jesper Danielsson
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Correspondence to Bejai R. Sarosh.

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Sarosh, B.R., Danielsson, J. & Meijer, J. Transcript profiling of oilseed rape (Brassica napus) primed for biocontrol differentiate genes involved in microbial interactions with beneficial Bacillus amyloliquefaciens from pathogenic Botrytis cinerea . Plant Mol Biol 70, 31–45 (2009). https://doi.org/10.1007/s11103-009-9455-4

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