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Rice phot1a mutation reduces plant growth by affecting photosynthetic responses to light during early seedling growth

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Abstract

The aim of this work was to characterize the phot1 mutant of rice during early seedling growth in various light conditions. We isolated the rice T-DNA insertion mutant phot1a-1 and compared it to the Tos17 insertion mutant phot1a-2. When phot1a mutants were grown under WL (100) and BL (40 μmol m−2 s−1), they demonstrated a considerable reduction in photosynthetic capacity, which included decreased leaf CO2 uptake and plant growth. Pigment analysis showed no significant difference between wild-type and mutants in the Chl a:b ratios, whereas in the latter, total concentration was reduced (a 2-fold decrease). Carotenoid contents of the mutants were also decreased considerably, implying the involvement of phot1a in pigment degradation. Deletion of phot1a showed higher contents of H2O2 in leaves. Chloroplastic APX and SOD activities were lower in the mutants whereas the activities of cytosolic enzymes were increased. Immunoblotting indicated reduced accumulation of photosystem proteins (D1, D2, CP43, Lhca2, and PsaC) relative to the other light-harvesting complexes in the mutant. We conclude that the defect of Os Phot1a affects degradation of chlorophylls and carotenoids, and under photosynthetically active photon fluxes, mutation of phot1a results in loss of photosynthetic capacity owing to the damage of photosystems caused by elevated H2O2 accumulation, leading to a reduction in plant growth.

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Abbreviations

APX:

Ascorbate peroxidase

BL:

Blue light

CAT:

Catalase

Chl:

Chlorophyll

Chl-APX:

Chloroplastic ascorbate peroxidase

ETR:

Electron transport rate

GUS:

β-glucuronidase

HPLC:

High performance liquid chromatography

H2O2 :

Hydrogen peroxide

NPQ:

Non-photochemical quenching

PS:

Photosystem(s)

Phot:

Phototropin

RT-PCR:

Reverse-transcriptase-polymerase chain reaction

ROS:

Reactive oxygen species

O •−2 :

Superoxide anion

Rubisco:

Ribulose-1,5-bisphosphate carboxylase/oxygenase

SOD:

Superoxide dismutase

WL:

White light

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Acknowledgments

We are grateful to Dr. Makoto Takano (NIAS, Japan) for providing seed materials of rice Phot1a-2. C–H Goh gratefully acknowledges the information of primers for phot1a-2 and critical discussion for the manuscript of Prof. Moritoshi Iino (Osaka City University, Japan) and Prof. Elizabeth Van Volkenburgh (University of Washington, USA). This work was supported by grants from the Korea Research Foundation (KRF-2004-015-C00532) and provided in part by Crop Functional Genomics Program (GC1111) to G.A.

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Authors and Affiliations

  1. Environmental Biotechnology and Research Center, Gyeongsang National University, Jinju, 660-701, South Korea

    Chang-Hyo Goh

  2. Research Institute for Basic Sciences, Cheju National University, Jeju, 690-756, South Korea

    Chang-Hyo Goh

  3. Department of Life Sciences, Pohang University of Science and Technology, Pohang, 790-784, South Korea

    Seonghoe Jang & Gynheung An

  4. Department of Wood Science and Technology, BK21 Program, Chonnam National University, Gwangju, 500-757, South Korea

    Sera Jung & Hyeun-Jong Bae

  5. Department of Molecular Biology, Pusan National University, Busan, 609-735, South Korea

    Ho-Seung Kim & Choon-Hwan Lee

  6. Department of Biology, Kyungpook National University, Daegu, 702-701, South Korea

    Hong-Gyu Kang

  7. Department of Biology, Chungnam National University, Taejon, 305-764, South Korea

    Youn-il Park

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  1. Chang-Hyo Goh
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  2. Seonghoe Jang
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Correspondence to Chang-Hyo Goh.

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Goh, CH., Jang, S., Jung, S. et al. Rice phot1a mutation reduces plant growth by affecting photosynthetic responses to light during early seedling growth. Plant Mol Biol 69, 605–619 (2009). https://doi.org/10.1007/s11103-008-9442-1

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  • DOI: https://doi.org/10.1007/s11103-008-9442-1

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