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Spatial distribution and hormonal regulation of gene products from methyl erythritol phosphate and monoterpene-secoiridoid pathways in Catharanthus roseus

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Abstract

The monoterpene indole alkaloids (MIAs) from Madagascar periwinkle (Catharanthus roseus) are secondary metabolites of high interest due to their therapeutical values. Secologanin, the monoterpenoid moiety incorporated into MIAs, is derived from the plastidial methyl-d-erythritol 4-phosphate (MEP) pathway. Here, we have cloned a cDNA encoding hydroxymethylbutenyl diphosphate synthase (HDS), a MEP pathway enzyme, and generated antibodies to investigate the distribution of transcripts and protein in MIA-producing aerial tissues. Consistent with our earlier work, transcripts for the genes encoding the so-called early steps in monoterpenoid biosynthesis (ESMB) enzymes (HDS, others MEP pathway enzymes and geraniol 10-hydroxylase) were preferentially co-localized to internal phloem associated parenchyma (IPAP) cells. By contrast, transcripts for the enzyme catalysing the last biosynthetic step to secologanin, secologanin synthase, were found in the epidermis. A coordinated response of ESMB genes was also observed in cell cultures stimulated to synthesise MIAs by hormone treatment, whereas no changes in SLS expression were detected under the same experimental conditions. Immunocytolabelling studies with the HDS-specific serum demonstrated the localisation of HDS to the plastid stroma and revealed that HDS proteins were most abundant in IPAP cells but could also be found in other cell types, including epidermal and mesophyll cells. Besides showing the existence of post-transcriptional mechanisms regulating the levels of HDS in C. roseus cells, our results support that intercellular translocation likely plays an important role during monoterpene-secoiridoid assembly.

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Abbreviations

DXS:

1-Deoxy-d-xylulose 5-phosphate synthase

DXR:

1-Deoxy-d-xylulose 5-phosphate reductoisomerase

ESMB:

Early steps in monoterpenoid biosynthesis

G10H:

Geraniol 10-hydroxylase

HDS:

Hydroxymethylbutenyl 4-diphosphate synthase

HMBPP:

Hydroxymethylbutenyl 4-diphosphate

IM:

Inducing medium

IPAP:

Internal phloem associated parenchyma

MECS:

2C-methyl-d-erythritol 2,4-diphosphate synthase

MeJa:

Methyljasmonate

MEP:

Methyl-d-erythritol 4-phosphate

MIA:

Monoterpene indole alkaloid

MM:

Maintenance medium

PM:

Production medium

SLS:

Secologanin synthase

STR:

Strictosidine synthase

T16H:

Tabersonine 16-hydroxylase

TEM:

Transmission electron microscopy

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Acknowledgements

This research was financially supported by the Ministère de l’Education Nationale, de la Recherche et de la Technologie (MENRT, France), by Biotechnocentre, by the Conseil Régional du Centre, by the Ligue contre le Cancer (comité d’Indre et Loire and comité de l’Indre) and by the Spanish Ministerio de Educacion y Ciencia and FEDER (grant BIO2005–00367 to MR-C). We thank Dr. J. Memelink (University of Leiden, the Netherlands) who kindly provided C. roseus G10H cDNA and the oriented cDNA library, and Dr. N. Campos (Universitat de Barcelona) for the EcAB3-3 strain. We also thank Dr. B. Arbeille and the staff of the Electron Microscopy Platform (CHU Bretonneau, Tours, France) for providing excellent working conditions for ultramicrotomy and TEM studies.

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Authors and Affiliations

  1. EA 2106 “Biomolécules et Biotechnologies Végétales”, UFR des Sciences Pharmaceutiques, Université François Rabelais de Tours, Tours, 37200, France

    Audrey Oudin

  2. EA 2106 “Biomolécules et Biotechnologies Végétales”, UFR des Sciences et Techniques, Université François Rabelais de Tours, Tours, 37200, France

    Samira Mahroug, Vincent Courdavault, Benoit St-Pierre & Vincent Burlat

  3. Centre de Biophysique Moléculaire, UPR 4301, CNRS, rue Charles Sadron, Orleans cedex 02, 45071, France

    Nadège Hervouet & Charles Zelwer

  4. Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 645, 08028, Barcelona, Spain

    Manuel Rodríguez-Concepción

  5. Unité sous Contrat reconnue par l’INRA “Facteurs de transcription et ingénierie métabolique végétale”, Université François Rabelais de Tours, Tours, France

    Samira Mahroug, Benoit St-Pierre & Vincent Burlat

  6. Consorci CSIC-IRTA de Genetica Molecular Vegetal, Jordi Girona 18-26, 08034, Barcelona, Spain

    Manuel Rodríguez-Concepción

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  1. Audrey Oudin
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Correspondence to Audrey Oudin.

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Oudin, A., Mahroug, S., Courdavault, V. et al. Spatial distribution and hormonal regulation of gene products from methyl erythritol phosphate and monoterpene-secoiridoid pathways in Catharanthus roseus . Plant Mol Biol 65, 13–30 (2007). https://doi.org/10.1007/s11103-007-9190-7

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