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The moss genes PpSKI1 and PpSKI2 encode nuclear SnRK1 interacting proteins with homologues in vascular plants

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Abstract

The yeast Snf1, animal AMPK, and plant SnRK1 protein kinases constitute a family of related proteins that have been proposed to serve as metabolic sensors of the eukaryotic cell. We have previously reported the characterization of two redundant SnRK1 encoding genes (PpSNF1a and PpSNF1b) in the moss Physcomitrella patens. Phenotypic analysis of the snf1a snf1b double knockout mutant suggested that SnRK1 is important for the plant’s ability to recognize and adapt to conditions of limited energy supply, and also suggested a possible role of SnRK1 in the control of plant development. We have now used a yeast two-hybrid system to screen for PpSnf1a interacting proteins. Two new moss genes were found, PpSKI1 and PpSKI2, which encode highly similar proteins with homologues in vascular plants. Fusions of the two encoded proteins to the green fluorescent protein localize to the nucleus. Knockout mutants for either gene have an excess of gametophores under low light conditions, and exhibit reduced gametophore stem lengths. Possible functions of the new proteins and their connection to the SnRK1 kinase are discussed.

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Abbreviations

AD:

Activation domain

AMPK:

AMP-activated kinase

cDNA:

complementary DNA

DBD:

DNA binding domain

EST:

expressed sequence tag

GFP:

green fluorescent protein

NLS:

nuclear localization signal

ORF:

open reading frame

PCR:

polymerase chain reaction

qRT-PCR:

quantitative real-time reverse transcriptase polymerase chain reaction

RT-PCR:

reverse transcriptase polymerase chain reaction

Ski:

Snf1-related kinase interactor

SnRK:

Snf1-related kinase

YTH:

yeast two hybrid

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Acknowledgements

This work was supported by grants from the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (Formas) and the Swedish Foundation for Strategic Research to HR, by a grant from the Carl Trygger Foundation to MT, and by a grant from the Uppsala Centre for Comparative Genomics to MT and HR.

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Author notes

  1. Pierre-Alain Girod

    Present address: Selexis S.A., Ch. des Aulx 18, CH-1228, Plan-les-Ouates, Geneva, Switzerland

  2. Didier G. Schaefer

    Present address: Institut Jean-Pierre Bourgin, SGAP, INRA Versailles, Route de Saint Cyr, F-78000, Versailles, France

Authors and Affiliations

  1. Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, P.O. Box 7080, 750 07, Uppsala, Sweden

    Mattias Thelander & Tina Olsson

  2. Department of Medical Biochemistry and Microbiology, Uppsala University, P.O. Box 582, SE-75123, Uppsala, Sweden

    Anders Nilsson, Monika Johansson & Hans Ronne

  3. Department of Plant Molecular Biology, University of Lausanne, CH 1015, Lausanne, Switzerland

    Pierre-Alain Girod, Didier G. Schaefer & Jean-Pierre Zrÿd

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  1. Mattias Thelander
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Correspondence to Mattias Thelander.

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Thelander, M., Nilsson, A., Olsson, T. et al. The moss genes PpSKI1 and PpSKI2 encode nuclear SnRK1 interacting proteins with homologues in vascular plants. Plant Mol Biol 64, 559–573 (2007). https://doi.org/10.1007/s11103-007-9176-5

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