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Molecular analysis of early rice stamen development using organ-specific gene expression profiling

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Abstract

Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and will be useful for crop improvement applications. Plant organ formation, or organogenesis, occurs when a group of primordial cells differentiates into an organ, through a well-orchestrated series of events, with a given shape, structure and function. Research over the past two decades has elucidated the molecular mechanisms of organ identity and dorsalventral axis determinations. However, little is known about the molecular mechanisms underlying the successive processes. To develop an effective approach for studying organ formation at the molecular level, we generated organ-specific gene expression profiles (GEPs) reflecting early development in rice stamen. In this study, we demonstrated that the GEPs are highly correlated with early stamen development, suggesting that this analysis is useful for dissecting stamen development regulation. Based on the molecular and morphological correlation, we found that over 26 genes, that were preferentially up-regulated during early stamen development, may participate in stamen development regulation. In addition, we found that differentially expressed genes during early stamen development are clustered into two clades, suggesting that stamen development may comprise of two distinct phases of pattern formation and cellular differentiation. Moreover, the organ-specific quantitative changes in gene expression levels may play a critical role for regulating plant organ formation.

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Abbreviations

aRNA:

antisense RNA

cDNA:

complementary DNA

DEPC:

diethyl pyrocarbonate

dUTP:

deoxyuridine triphosphate

EMS:

EXCESS MICROSPOROCYTES

ESTs:

expression sequence tags

EXS:

EXTRA SPOROGENOUS CELLS

FIL:

FILAMENTOUS FLOWER

LLS:

LETHAL LEAF SPOT

LPA:

linear polyacrylamide

msca1 :

male sterile converted anther1

NZZ:

NOZZLE

OsMADS7:

rice MADS-box protein 7

PCR:

polymerase chain reaction

PHD:

plant homeodomain

RNA:

ribosome nucleic acid

RT:

reverse transcription

SPL:

SPOROCYTELESS

TPD:

TAPETUM DETERMINANT

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Authors and Affiliations

  1. PKU-Yale Joint Research Center of Agricultural and Plant Molecular Biology, National Key Laboratory of Protein Engineering and Plant Gene Engineering, College of Life Sciences, Peking Unviersity, Beijing, 100871, China

    Xiao-Chun Lu, Hua-Qin Gong, Mo-Li Huang, Su-Lan Bai, Xizeng Mao, Song-Gang Li, Liping Wei, Jie-Shuai Yuwen, Zhi-Hong Xu & Shu-Nong Bai

  2. Department of Probability and Statistics, School of Mathematical Sciences, Peking University, Beijing, 100871, China

    Yang-Bo He & Zhi Geng

  3. National Plant Gene Research Center (Beijing), Beijing, 100871, China

    Shu-Nong Bai

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  1. Xiao-Chun Lu
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  2. Hua-Qin Gong
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  3. Mo-Li Huang
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  4. Su-Lan Bai
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  9. Liping Wei
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  12. Shu-Nong Bai
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Correspondence to Shu-Nong Bai.

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Xiao-Chun Lu, Hua-Qin Gong contributed equally to this work.

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Lu, XC., Gong, HQ., Huang, ML. et al. Molecular analysis of early rice stamen development using organ-specific gene expression profiling. Plant Mol Biol 61, 845–861 (2006). https://doi.org/10.1007/s11103-006-0054-3

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  • DOI: https://doi.org/10.1007/s11103-006-0054-3

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