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Characterizations of a Hypomorphic Argonaute1 Mutant Reveal Novel AGO1 Functions in Arabidopsis Lateral Organ Development

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Abstract

Genes that encode the ARGONAUTE (AGO) proteins make up a highly conserved family, and several members in the family have been defined to function in posttranscriptional gene silencing (PTGS) in plants, quelling in fungi and RNAi in animals. The Arabidopsis AGO1 gene has been demonstrated to be crucial in multiple RNA silencing pathways (PTGS, microRNA and trans-acting siRNA pathways); however, its biological functions do not seem to be fully addressed. Here we report characterizations of a new hypomorphic ago1 allele, ago1-37, and show novel AGO1 functions important in lateral organ development. We found that double mutants combining ago1-37 with asymmetric leaves1 (as1) or asymmetric leaves2 (as2) produced more severe phenotypes than the single mutants, indicating that AGO1 genetically interacts with AS1 and AS2 for plant development. Similar to the previously characterized mutants rdr6, sgs3 and zippy, which are deficient in the trans-acting siRNA activity, ago1-37 also showed an earlier phase transition from juvenile to adult leaves. Moreover, based on the detailed phenotypic analyses of single and double mutant plants, we found that the AGO1 functions are required for repressing several class I KNOTTED1-like homeobox (KNOX) genes in leaves, and for specifying both adaxial and abaxial identities of the leaf and petal. Our results demonstrate several important AGO1 functions in plant lateral organ development.

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Abbreviations

PTGS:

posttranscriptional gene silencing

RdRP:

RNA-dependent RNA polymerase

RISC:

RNA induced silencing complex

References

  • N. Baumberger D.C. Baulcombe Y. Qi A.M. Denli G.J. Hannon (2005) ArticleTitleArabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits microRNAs and short interfering RNAs Proc. Natl. Acad. Sci. USA 19 421–428

    Google Scholar 

  • E. Bernstein A.A. Caudy S.M. Hammond G.J. Hannon (2001) ArticleTitleRole for a bidentate ribonuclease in the initiation step of RNA interference Nature 409 363–366 Occurrence Handle11201747 Occurrence Handle1:CAS:528:DC%2BD3MXms12ksA%3D%3D Occurrence Handle10.1038/35053110

    Article  PubMed  CAS  Google Scholar 

  • K. Bohmert I. Camus C. Bellini D. Bouchez M. Caboche C. Benning (1998) ArticleTitleAGO1 defines a novel locus of Arabidopsis controlling leaf development EMBO J. 17 170–180 Occurrence Handle9427751 Occurrence Handle1:CAS:528:DyaK1cXnvVyisA%3D%3D Occurrence Handle10.1093/emboj/17.1.170

    Article  PubMed  CAS  Google Scholar 

  • J.L. Bowman Y. Eshed S.F. Baum (2002) ArticleTitleEstablishment of polarity in angiosperm lateral organs Trends Genet. 18 134–141 Occurrence Handle11858837 Occurrence Handle1:CAS:528:DC%2BD38XhtlGju70%3D Occurrence Handle10.1016/S0168-9525(01)02601-4

    Article  PubMed  CAS  Google Scholar 

  • M.E. Byrne R. Barley M. Curtis J.M. Arroyo M. Dunham A. Hudson R.A. Martienssen (2000) ArticleTitleASYMMETRIC LEAVES1 mediates leaf patterning and stem cell function in Arabidopsis Nature 408 967–971 Occurrence Handle11140682 Occurrence Handle1:CAS:528:DC%2BD3MXhslSqtA%3D%3D Occurrence Handle10.1038/35050091

    Article  PubMed  CAS  Google Scholar 

  • M.E. Byrne J. Simorowski R.A. Martienssen (2002) ArticleTitleASYMMETRIC LEAVES1 reveals knox gene redundancy in Arabidopsis Development 129 1957–1965 Occurrence Handle11934861 Occurrence Handle1:CAS:528:DC%2BD38XjslSls74%3D

    PubMed  CAS  Google Scholar 

  • C. Catalanotto G. Azzalin G. Macino C. Cogoni (2002) ArticleTitleInvolvement of small RNAs and role of the qde genes in the gene silencing pathway in Neurospora Genes Dev. 16 790–795 Occurrence Handle11937487 Occurrence Handle1:CAS:528:DC%2BD38XivFKjt7g%3D Occurrence Handle10.1101/gad.222402

    Article  PubMed  CAS  Google Scholar 

  • A.A. Caudy M. Myers G.J. Hannon S.M. Hammond (2002) ArticleTitleFragile X-related protein and VIG associate with the RNA interference machinery Genes Dev. 16 2491–2496 Occurrence Handle12368260 Occurrence Handle1:CAS:528:DC%2BD38XnvVGjt7w%3D Occurrence Handle10.1101/gad.1025202

    Article  PubMed  CAS  Google Scholar 

  • C. Chen S. Wang H. Huang (2000) ArticleTitleLEUNIG has multiple functions in gynoecium development in Arabidopsis Genesis 26 42–54 Occurrence Handle10660672 Occurrence Handle1:CAS:528:DC%2BD3cXhtlCgt7Y%3D Occurrence Handle10.1002/(SICI)1526-968X(200001)26:1<42::AID-GENE7>3.0.CO;2-J

    Article  PubMed  CAS  Google Scholar 

  • G. Chuck C. Lincoln S. Hake (1996) ArticleTitleKNAT1 induces lobed leaves with ectopic meristems when overexpressed in Arabidopsis Plant Cell 9 1277–1289 Occurrence Handle10.1105/tpc.8.8.1277

    Article  Google Scholar 

  • D.E. Cikaluk N. Tahbaz L.C. Hendricks G.E. DiMattia D. Hansen D. Pilgrim T.C. Hobman (1999) ArticleTitleGERp95, a membrane-associated protein that belongs to a family of proteins involved in stem cell differentiation Mol. Biol. Cell 10 3357–3372 Occurrence Handle10512872 Occurrence Handle1:CAS:528:DyaK1MXmvFaisbw%3D

    PubMed  CAS  Google Scholar 

  • C. Cogoni J.T. Irelan M. Schumacher T. Schmidhauser E.U. Selker G. Macino (1996) ArticleTitleTransgene silencing of the al-1 gene in vegetative cells of Ueurospora is mediated by a cytoplasmic effector and does not depend on DNA-DNA interactions or DNA methylation EMBO J. 15 3153–3163 Occurrence Handle8670816 Occurrence Handle1:CAS:528:DyaK28XjvFCgtb4%3D

    PubMed  CAS  Google Scholar 

  • J.G. Doench C.P. Petersen P.A. Sharp (2003) ArticleTitlesiRNAs can function as miRNAs Genes Dev. 17 438–442 Occurrence Handle12600936 Occurrence Handle1:CAS:528:DC%2BD3sXhslCqtbo%3D Occurrence Handle10.1101/gad.1064703

    Article  PubMed  CAS  Google Scholar 

  • K. Endrizzi B. Moussian A. Haecker J.Z. Levin T. Laux (1996) ArticleTitleThe SHOOT MERISTEMLESS gene is required for maintenance of undifferentiated cells in Arabidopsis shoot and floral meristems and acts at a different regulatory level than the meristem genes WUSCHEL and ZWILLE Plant J. 10 967–979 Occurrence Handle9011081 Occurrence Handle1:CAS:528:DyaK2sXnsV2ltQ%3D%3D Occurrence Handle10.1046/j.1365-313X.1996.10060967.x

    Article  PubMed  CAS  Google Scholar 

  • M. Fagard S. Boutet J.B. Morel C. Bellini H. Vaucheret (2000) ArticleTitleAGO1, QDE-2, and RDE-1 are related proteins required for post-transcriptional gene silencing in plants, quelling in fungi, and RNA interference in animals Proc. Natl. Acad. Sci. USA 97 11650–11654 Occurrence Handle11016954 Occurrence Handle1:CAS:528:DC%2BD3cXnsF2qtr0%3D Occurrence Handle10.1073/pnas.200217597

    Article  PubMed  CAS  Google Scholar 

  • A. Fire (1999) ArticleTitleRNA-triggered gene silencing Trends Genet. 15 358–363 Occurrence Handle10461204 Occurrence Handle1:CAS:528:DyaK1MXltlyms7s%3D Occurrence Handle10.1016/S0168-9525(99)01818-1

    Article  PubMed  CAS  Google Scholar 

  • A. Grishok A.E. Pasquinelli D. Conte N. Li S. Parrish I. Ha D.L. Baillie A. Fire G. Ruvkun C.C. Mello (2001) ArticleTitleGenes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing Cell 106 23–34 Occurrence Handle11461699 Occurrence Handle1:CAS:528:DC%2BD3MXlsVCqtrw%3D Occurrence Handle10.1016/S0092-8674(01)00431-7

    Article  PubMed  CAS  Google Scholar 

  • C.M. Ha G.T. Kim B.C. Kim J.H. Jun M.S. Soh Y. Ueno Y. Machida H. Tsukaya H.G. Nam (2003) ArticleTitleThe BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis Development 130 161–172 Occurrence Handle12441300 Occurrence Handle1:CAS:528:DC%2BD3sXntVCmsA%3D%3D Occurrence Handle10.1242/dev.00196

    Article  PubMed  CAS  Google Scholar 

  • S.M. Hammond E. Bernstein D. Beach G.J. Hannon (2000) ArticleTitleAn RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells Nature 404 293–296 Occurrence Handle10749213 Occurrence Handle1:CAS:528:DC%2BD3cXit1agtrw%3D Occurrence Handle10.1038/35005107

    Article  PubMed  CAS  Google Scholar 

  • S.M. Hammond S. Boettcher A.A. Caudy R. Kobayashi G.J. Hannon (2001a) ArticleTitleArgonaute2, a link between genetic and biochemical analyses of RNAi Science 293 1146–1150 Occurrence Handle1:CAS:528:DC%2BD3MXmtVWqtLo%3D Occurrence Handle10.1126/science.1064023

    Article  CAS  Google Scholar 

  • S.M. Hammond A.A. Caudy G.J. Hannon (2001b) ArticleTitlePost-transcriptional gene silencing by double-stranded RNA Nat. Rev. Genet. 2 110–119 Occurrence Handle1:CAS:528:DC%2BD3MXisVGjtbk%3D Occurrence Handle10.1038/35052556

    Article  CAS  Google Scholar 

  • C. Hunter H. Sun R.S. Poethig (2003) ArticleTitleThe Arabidopsis heterochronic gene ZIPPY is an ARGONAUTE family member Curr. Biol. 13 1734–1749 Occurrence Handle14521841 Occurrence Handle1:CAS:528:DC%2BD3sXnvVOmtbk%3D Occurrence Handle10.1016/j.cub.2003.09.004

    Article  PubMed  CAS  Google Scholar 

  • H. Iwakawa Y. Ueno E. Semiarti H. Onouchi S. Kojima H. Tsukaya M. Hasebe T. Soma M. Ikezaki C. Machida Y. Machida (2002) ArticleTitleThe ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana, required for formation of a symmetric flat lamina, encodes a member of a novel family of proteins characterized by cystein repeats and a luecine zipper Plant Cell Physiol. 43 467–478 Occurrence Handle12040093 Occurrence Handle1:CAS:528:DC%2BD38XktFWgtrY%3D Occurrence Handle10.1093/pcp/pcf077

    Article  PubMed  CAS  Google Scholar 

  • Y. Kataoka M. Takeichi T. Uemura (2001) ArticleTitleDevelopmental roles and molecular characterization of a Drosophila homologue of Arabidopsis Argonaute1, the founder of a novel gene superfamily Genes Cells 6 313–325 Occurrence Handle11318874 Occurrence Handle1:CAS:528:DC%2BD3MXjvVWgs7k%3D Occurrence Handle10.1046/j.1365-2443.2001.00427.x

    Article  PubMed  CAS  Google Scholar 

  • A. Katz M. Oliva A. Mosquna O. Hakim N. Ohad W.C. Lin B. Shuai P.S. Springer V. Pautot J. Dockx O. Hamant J. Kronenberger O. Grandjean D. Jublot J. Traas C. Lincoln J. Long J. Yamaguchi K. Serikawa S. Hake (2004) ArticleTitleFIE and CURLY LEAF polycomb proteins interact in the regulation of homeobox gene expression during sporophyte development Plant J. 37 707–719 Occurrence Handle14871310 Occurrence Handle1:CAS:528:DC%2BD2cXislylt74%3D Occurrence Handle10.1111/j.1365-313X.2003.01996.x

    Article  PubMed  CAS  Google Scholar 

  • R.A. Kerstetter K. Bollman R.A. Taylor K. Bomblies R.S. Poethig (2001) ArticleTitleKANADI regulates organ polarity in Arabidopsis Nature 411 706–709 Occurrence Handle11395775 Occurrence Handle1:CAS:528:DC%2BD3MXksF2gur8%3D Occurrence Handle10.1038/35079629

    Article  PubMed  CAS  Google Scholar 

  • C.A. Kidner R.A. Martienssen (2004) ArticleTitleSpatially restricted microRNA directs leaf polarity through ARGONAUTE1 Nature 428 81–84 Occurrence Handle14999284 Occurrence Handle1:CAS:528:DC%2BD2cXhslCnsLw%3D Occurrence Handle10.1038/nature02366

    Article  PubMed  CAS  Google Scholar 

  • C.A. Kidner R.A. Martienssen (2005) ArticleTitleThe role of ARGONAUTE1 (AGO1) in meristem formation and identity Dev. Biol. 280 504–517 Occurrence Handle15882589 Occurrence Handle1:CAS:528:DC%2BD2MXjvVGksb0%3D Occurrence Handle10.1016/j.ydbio.2005.01.031

    Article  PubMed  CAS  Google Scholar 

  • M.K. Kumaran J.L. Bowman V. Sundaresan (2002) ArticleTitleYABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis Plant Cell 14 2761–2770 Occurrence Handle12417699 Occurrence Handle1:CAS:528:DC%2BD38XovF2qur8%3D Occurrence Handle10.1105/tpc.004911

    Article  PubMed  CAS  Google Scholar 

  • H. Li L. Xu H. Wang Z. Yuan X. Cao Z. Yang D. Zhang Y. Xu H. Huang (2005) ArticleTitleThe putative RNA-Dependent RNA Polymerase RDR6 acts synergistically with ASYMMETRIC LEAVES1 and 2 to repress BREVIPEDICELLUS and MicroRNA165/166 in Arabidopsis leaf development Plant Cell 17 2157–2171 Occurrence Handle16006579 Occurrence Handle1:CAS:528:DC%2BD2MXpsFGjs7g%3D Occurrence Handle10.1105/tpc.105.033449

    Article  PubMed  CAS  Google Scholar 

  • W.C. Lin B. Shuai P.S. Springer (2003) ArticleTitleThe Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial–abaxial patterning Plant Cell 15 2241–2252 Occurrence Handle14508003 Occurrence Handle1:CAS:528:DC%2BD3sXotlGmt7k%3D Occurrence Handle10.1105/tpc.014969

    Article  PubMed  CAS  Google Scholar 

  • A. Lingel B. Simon E. Izaurralde M. Sattler (2003) ArticleTitleStructure and nucleic-acid binding of the Drosophila Argonaute 2 PAZ domain Nature 426 465–469 Occurrence Handle14615801 Occurrence Handle1:CAS:528:DC%2BD3sXpt1Gls7c%3D Occurrence Handle10.1038/nature02123

    Article  PubMed  CAS  Google Scholar 

  • A. Lingel B. Simon E. Izaurralde M. Sattler (2004) ArticleTitleNMR assignment of the Drosophila Argonaute2 PAZ domain J. Biomol. NMR 29 421–422 Occurrence Handle15213449 Occurrence Handle1:CAS:528:DC%2BD2cXltVGntL4%3D Occurrence Handle10.1023/B:JNMR.0000032513.15693.04

    Article  PubMed  CAS  Google Scholar 

  • J. Liu M.A. Carmell F.V. Rivas C.G. Marsden J.M. Thomson J.J. Song S.M. Hammond L. Joshua-Tor G.J. Hannon (2004) ArticleTitleArgonaute2 is the catalytic engine of mammalian RNAi Science 305 1437–1441 Occurrence Handle15284456 Occurrence Handle1:CAS:528:DC%2BD2cXntFartLk%3D Occurrence Handle10.1126/science.1102513

    Article  PubMed  CAS  Google Scholar 

  • C. Llave K.D. Kasschau M.A. Rector J.C. Carrington (2002) ArticleTitleEndogenous and silencing-associated small RNAs in plants Plant Cell 14 1605–1619 Occurrence Handle12119378 Occurrence Handle1:CAS:528:DC%2BD38XlvV2rsbY%3D Occurrence Handle10.1105/tpc.003210

    Article  PubMed  CAS  Google Scholar 

  • J.A. Long M.K. Barton (1998) ArticleTitleThe development of apical embryonic pattern in Arabidopsis Development 125 3027–3035 Occurrence Handle9671577 Occurrence Handle1:CAS:528:DyaK1cXmtVaht7g%3D

    PubMed  CAS  Google Scholar 

  • K. Lynn A. Fernandez M. Aida J. Sedbrook M. Tasaka P. Masson M.K. Barton (1999) ArticleTitleThe PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene Development 126 469–481 Occurrence Handle9876176 Occurrence Handle1:CAS:528:DyaK1MXhsVSlsbw%3D

    PubMed  CAS  Google Scholar 

  • E.V. Makeyev D.H. Bamford (2002) ArticleTitleCellular RNA-dependent RNA polymerase involved in posttranscriptional gene silencing has two distinct activity modes Mol. Cell 10 1417–1427 Occurrence Handle12504016 Occurrence Handle1:CAS:528:DC%2BD3sXjslOitg%3D%3D Occurrence Handle10.1016/S1097-2765(02)00780-3

    Article  PubMed  CAS  Google Scholar 

  • M.A. Matzke A.J. Matzke G.J. Pruss V.B. Vance (2001) ArticleTitleRNA-based silencing strategies in plants Curr. Opin. Genet. Dev. 11 221–227 Occurrence Handle11250148 Occurrence Handle1:CAS:528:DC%2BD3MXis1aku7s%3D Occurrence Handle10.1016/S0959-437X(00)00183-0

    Article  PubMed  CAS  Google Scholar 

  • J.R. McConnell M.K. Barton (1998) ArticleTitleLeaf polarity and meristem formation in Arabidopsis Development 125 2935–2942 Occurrence Handle9655815 Occurrence Handle1:CAS:528:DyaK1cXls1Snu7w%3D

    PubMed  CAS  Google Scholar 

  • J.R. McConnell J. Emery Y. Eshed N. Bao J. Bowman M.K. Barton (2001) ArticleTitleRole of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots Nature 411 709–713 Occurrence Handle11395776 Occurrence Handle1:CAS:528:DC%2BD3MXksF2ns7w%3D Occurrence Handle10.1038/35079635

    Article  PubMed  CAS  Google Scholar 

  • J.B. Morel C. Godon P. Mourrain C. Beclin S. Boutet F. Feuerbach F. Proux H. Vaucheret (2002) ArticleTitleFertile hypomorphic ARGONAUTE ago1 mutants impaired in post-transcriptional gene silencing and virus resistance Plant Cell 14 629–639 Occurrence Handle11910010 Occurrence Handle1:CAS:528:DC%2BD38XivVegtLw%3D Occurrence Handle10.1105/tpc.010358

    Article  PubMed  CAS  Google Scholar 

  • N. Muangsan C. Beclin H. Vaucheret D. Robertson (2004) ArticleTitleGeminivirus VIGS of endogenous genes requires SGS2/SDE1 and SGS3 and defines a new branch in the genetic pathway for silencing in plants Plant J. 38 1004–1014 Occurrence Handle15165191 Occurrence Handle1:CAS:528:DC%2BD2cXlslCntL4%3D Occurrence Handle10.1111/j.1365-313X.2004.02103.x

    Article  PubMed  CAS  Google Scholar 

  • K.L. Newman A.G. Fernandez M.K. Barton (2002) ArticleTitleRegulation of axis determinacy by the Arabidopsis PINHEAD Plant Cell 14 3029–3042 Occurrence Handle12468725 Occurrence Handle1:CAS:528:DC%2BD3sXhtFCmtw%3D%3D Occurrence Handle10.1105/tpc.005132

    Article  PubMed  CAS  Google Scholar 

  • N. Ori Y. Eshed G. Chuck J.L. Bowman S. Hake (2000) ArticleTitleMechanisms that control KNOX gene expression in the Arabidopsis shoot Development 127 5523–5532 Occurrence Handle11076771 Occurrence Handle1:CAS:528:DC%2BD3MXltFWkug%3D%3D

    PubMed  CAS  Google Scholar 

  • M. Pal-Bhadra U. Bhadra J.A. Birchler (2002) ArticleTitleRNAi related mechanisms affect both transcriptional and posttranscriptional transgene silencing in Drosophila Mol. Cell 9 315–327 Occurrence Handle11864605 Occurrence Handle1:CAS:528:DC%2BD38XhvFKhu7k%3D Occurrence Handle10.1016/S1097-2765(02)00440-9

    Article  PubMed  CAS  Google Scholar 

  • C.W. Park Z. Chen B.T. Kren C.J. Steer (2004) ArticleTitleDouble-stranded siRNA targeted to the huntingtin gene does not induce DNA methylation Biochem. Biophys. Res. Commun. 323 275–280 Occurrence Handle15351733 Occurrence Handle1:CAS:528:DC%2BD2cXntlOitLg%3D Occurrence Handle10.1016/j.bbrc.2004.08.096

    Article  PubMed  CAS  Google Scholar 

  • J.S. Parker S.M. Roe D. Barford (2004) ArticleTitleCrystal structure of a PIWI protein suggests mechanisms for siRNA recognition and slicer activity EMBO J. 23 4727–4737 Occurrence Handle15565169 Occurrence Handle1:CAS:528:DC%2BD2cXhtVGitrzN Occurrence Handle10.1038/sj.emboj.7600488

    Article  PubMed  CAS  Google Scholar 

  • A. Peragine M. Yoshikawa G. Wu H.L. Albrecht R.S. Poethig (2004) ArticleTitleSGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis Genes Dev. 18 2368–2379 Occurrence Handle15466488 Occurrence Handle1:CAS:528:DC%2BD2cXot1yrtbo%3D Occurrence Handle10.1101/gad.1231804

    Article  PubMed  CAS  Google Scholar 

  • Y. Qi Y. Sun L. Xu Y. Xu H. Huang (2004) ArticleTitleERECTA is required for protection against heat-stress in the AS1/AS2 pathway to regulate adaxial–abaxial leaf polarity in Arabidopsis Planta 219 270–276 Occurrence Handle15034716 Occurrence Handle1:CAS:528:DC%2BD2cXksVSgsLY%3D Occurrence Handle10.1007/s00425-004-1248-z

    Article  PubMed  CAS  Google Scholar 

  • T.A. Rand K. Ginalski N.V. Grishin X. Wang J. Liu M.A. Carmell F.V. Rivas C.G. Marsden J.M. Thomson J.J. Song S.M. Hammond L. Joshua-Tor G.J. Hannon S.K. Smith J.B. Ma K. Ye D.J. Patel N.H. Tolia J. Schneiderman R.A. Martienssen K.S. Yan S. Yan A. Farooq A. Han L. Zeng M.M. Zhou A. Lingel B. Simon E. Izaurralde M. Sattler (2004) ArticleTitleBiochemical identification of Argonaute 2 as the sole protein required for RNA-induced silencing complex activity Proc. Natl. Acad. Sci. USA 101 14385–14389 Occurrence Handle15452342 Occurrence Handle1:CAS:528:DC%2BD2cXovVertLc%3D Occurrence Handle10.1073/pnas.0405913101

    Article  PubMed  CAS  Google Scholar 

  • E. Semiarti Y. Ueno H. Tsukaya H. Iwakawa C. Machida Y. Machida (2001) ArticleTitleThe ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves Development 128 1771–1783 Occurrence Handle11311158 Occurrence Handle1:CAS:528:DC%2BD3MXktFyjt7c%3D

    PubMed  CAS  Google Scholar 

  • K.R. Siegfried Y. Eshed S.F. Baum D. Otsuga G.N. Drews J. Bowman (1999) ArticleTitleMembers of the YABBY gene family specify abaxial cell fate in Arabidopsis Development 126 4117–4128 Occurrence Handle10457020 Occurrence Handle1:CAS:528:DyaK1MXmsleqsbY%3D

    PubMed  CAS  Google Scholar 

  • A. Sigova N. Rhind P.D. Zamore (2004) ArticleTitleA single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombe Genes Dev. 18 2359–2367 Occurrence Handle15371329 Occurrence Handle1:CAS:528:DC%2BD2cXot1yrtbw%3D Occurrence Handle10.1101/gad.1218004

    Article  PubMed  CAS  Google Scholar 

  • J.J. Song J. Liu N.H. Tolia J. Schneiderman S.K. Smith R.A. Martienssen G.J. Hannon L. Joshua-Tor (2003) ArticleTitleThe crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes Nat. Struct. Biol. 10 26–32 Occurrence Handle10.1038/nsb1016

    Article  Google Scholar 

  • J.J. Song S.K. Smith G.J. Hannon L. Joshua-Tor (2004) ArticleTitleCrystal structure of Argonaute and its implications for RISC slicer activity Science 305 1434–1437 Occurrence Handle15284453 Occurrence Handle1:CAS:528:DC%2BD2cXntFartLs%3D Occurrence Handle10.1126/science.1102514

    Article  PubMed  CAS  Google Scholar 

  • C. Sorin J.D. Bussell I. Camus K. Ljung M. Kowalczyk G. Geiss H. McKhann C. Garcion H. Vaucheret G. Sandberg C. Bellini (2005) ArticleTitleAuxin and light control of adventitious rooting in Arabidopsis require ARGONAUTE1 Plant Cell 17 1343–1359 Occurrence Handle15829601 Occurrence Handle1:CAS:528:DC%2BD2MXksVKksrs%3D Occurrence Handle10.1105/tpc.105.031625

    Article  PubMed  CAS  Google Scholar 

  • Y. Sun W. Zhang F. Li Y. Guo T. Liu H. Huang (2000) ArticleTitleIdentification and genetic mapping of four novel genes that regulate leaf development in Arabidopsis Cell Res. 10 325–335 Occurrence Handle10.1038/sj.cr.7290059

    Article  Google Scholar 

  • Y. Sun Q. Zhou W. Zhang Y. Fu H. Huang (2002) ArticleTitleASYMMETRIC LEAVES1, an Arabidopsis gene that is involved in the control of cell differentiation in leaves Planta 214 694–702 Occurrence Handle11882937 Occurrence Handle1:CAS:528:DC%2BD38Xhs1yqs7k%3D Occurrence Handle10.1007/s004250100673

    Article  PubMed  CAS  Google Scholar 

  • H. Tabara M. Sarkissian W.G. Kelly J. Fleenor A. Grishok L. Timmons A. Fire C.C. Mello (1999) ArticleTitleThe rde-1 gene, RNA interference, and transposon silencing in C. elegans Cell 99 123–132 Occurrence Handle10535731 Occurrence Handle1:CAS:528:DyaK1MXmvFeltb4%3D Occurrence Handle10.1016/S0092-8674(00)81644-X

    Article  PubMed  CAS  Google Scholar 

  • A. Telfer K.M. Bollman R.S. Poethig (1997) ArticleTitlePhase change and the regulation of trichome distribution in Arabidopsis thaliana Development 124 645–654 Occurrence Handle9043079 Occurrence Handle1:CAS:528:DyaK2sXhtlersbw%3D

    PubMed  CAS  Google Scholar 

  • F.E. Vaistij L. Jones et al. (2002) ArticleTitleSpreading of RNA targeting and DNA methylation in RNA silencing requires transcription of the target gene and a putative RNA-Dependent RNA Polymerase Plant Cell 14 857–867 Occurrence Handle11971140 Occurrence Handle1:CAS:528:DC%2BD38XjsFWktr8%3D Occurrence Handle10.1105/tpc.010480

    Article  PubMed  CAS  Google Scholar 

  • H. Vaucheret C. Beclin M. Fagard (2001) ArticleTitlePost-transcriptional gene silencing in plants J. Cell. Sci. 114 3083–3091 Occurrence Handle11590235 Occurrence Handle1:CAS:528:DC%2BD3MXnsFOhtbo%3D

    PubMed  CAS  Google Scholar 

  • H. Vaucheret F. Vazquez P. Crete D.P. Bartel (2004) ArticleTitleThe action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development Genes Dev. 18 1187–1197 Occurrence Handle15131082 Occurrence Handle1:CAS:528:DC%2BD2cXktlCqs7s%3D Occurrence Handle10.1101/gad.1201404

    Article  PubMed  CAS  Google Scholar 

  • F. Vazquez H. Vaucheret R. Rajagopalan C. Lepers V. Gasciolli A.C. Mallory J.L. Hilbert D.P. Bartel P. Crete (2004) ArticleTitleEndogenous trans-Acting siRNAs regulate the accumulation of Arabidopsis mRNAs Mol. Cell 16 69–79 Occurrence Handle15469823 Occurrence Handle1:CAS:528:DC%2BD2cXptVKqtr4%3D Occurrence Handle10.1016/j.molcel.2004.09.028

    Article  PubMed  CAS  Google Scholar 

  • R.W. Williams G.M. Rubin (2002) ArticleTitleARGONAUTE1 is required for efficient RNA interference in Drosophila embryos Proc. Natl. Acad. Sci. USA 99 6889–6894 Occurrence Handle12011447 Occurrence Handle1:CAS:528:DC%2BD38XjvFCru7k%3D Occurrence Handle10.1073/pnas.072190799

    Article  PubMed  CAS  Google Scholar 

  • L. Xu Y. Xu A. Dong Y. Sun L. Pi H. Huang (2003) ArticleTitleNovel as1 and as2 defects in leaf adaxial–abaxial polarity reveal the requirement for ASYMMETRIC LEAVES1 and 2 and ERECTA functions in specifying leaf adaxial identity Development 130 4097–4107 Occurrence Handle12874130 Occurrence Handle1:CAS:528:DC%2BD3sXntFejs78%3D Occurrence Handle10.1242/dev.00622

    Article  PubMed  CAS  Google Scholar 

  • Y. Xu Y. Sun W. Liang H. Huang (2002) ArticleTitleThe Arabidopsis AS2 gene encoding a predicted leucine-zipper protein is required for the leaf polarity formation Acta. Bot. Sin. 44 1194–1202 Occurrence Handle1:CAS:528:DC%2BD2cXnslWgurc%3D

    CAS  Google Scholar 

  • K.S. Yan S. Yan A. Farooq A. Han L. Zeng M.M. Zhou (2003) ArticleTitleStructure and conserved RNA binding of the PAZ domain Nature 426 468–474 Occurrence Handle14615802 Occurrence Handle10.1038/nature02129

    Article  PubMed  Google Scholar 

  • D. Zilberman X. Cao S.E. Jacobsen (2003) ArticleTitleARGONAUTE4 control of locus-specific siRNA accumulation and DNA and histone methylation Science 299 716–719 Occurrence Handle12522258 Occurrence Handle1:CAS:528:DC%2BD3sXntFWhsg%3D%3D Occurrence Handle10.1126/science.1079695

    Article  PubMed  CAS  Google Scholar 

  • D. Zilberman X. Cao L.K. Johansen Z. Xie J.C. Carrington S.E. Jacobsen (2004) ArticleTitleRole of Arabidopsis ARGONAUTE4 in RNA-directed DNA methylation triggered by inverted repeats Curr. Biol. 14 1214–1220 Occurrence Handle15242620 Occurrence Handle1:CAS:528:DC%2BD2cXlsFyrt7k%3D Occurrence Handle10.1016/j.cub.2004.06.055

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China

    Li Yang, Weihua Huang, Hua Wang & Hai Huang

  2. College of Life Science and Biotechnology, Shanghai Jiao Tong University, 1954 Hua Shan Road, Shanghai, 200030, China

    Li Yang & Yuquan Xu

  3. School of Agriculture and Biology, Shanghai Jiao Tong University, 2678 Qi Xing Road, Shanghai, 201101, China

    Weihua Huang & Run Cai

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  1. Li Yang
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  2. Weihua Huang
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  3. Hua Wang
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  4. Run Cai
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  5. Yuquan Xu
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  6. Hai Huang
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Correspondence to Hai Huang.

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Li Yang, Weihua Huang: These authors contributed equally to this paper.

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Yang, L., Huang, W., Wang, H. et al. Characterizations of a Hypomorphic Argonaute1 Mutant Reveal Novel AGO1 Functions in Arabidopsis Lateral Organ Development. Plant Mol Biol 61, 63–78 (2006). https://doi.org/10.1007/s11103-005-5992-7

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