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DcMYB1 Acts as a Transcriptional Activator of the Carrot Phenylalanine Ammonia-lyase Gene (DcPAL1) in Response to Elicitor Treatment, UV-B Irradiation and the Dilution Effect

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Abstract

Expression of a carrot phenylalanine ammonia-lyase (PAL) gene (DcPAL1) in suspension-cultured carrot cells is induced by treatment with a fungal elicitor, ultraviolet B (UV-B) irradiation, and by transferring and diluting cells with fresh medium (the dilution effect). Box-L-like sequences are known as important cis-elements of genes for enzymes involved in the phenylpropanoid biosynthetic pathway. Six sequences, box-L0 to box-L5, exist in the DcPAL1 gene promoter region. In this study, we isolated cDNA encoding the R2R3 type of MYB transcription factor, DcMYB1, using yeast one-hybrid screening with box-L1 or box-L5 as target elements. DcMYB1 bound to boxes-L0, L1, L3/4, and L5 sequences (ACC(A/T)(A/T)CC) in␣vitro, and in yeast cells and carrot protoplasts. Transient expression of DcMYB1 could up-regulate DcPAL1 promoter activity in carrot protoplasts. Results of the transient expression experiment for the deletion-mutated promoters of boxes-L0, L1, L3, and L5 suggest that these box-L-like sequences were required for the complete activation of the DcPAL1 promoter by DcMYB1. Expression of DcMYB1 transcripts was induced 0.5 h after elicitor treatment or UV-B irradiation, and 2 h after the dilution effect. Induction of DcPAL1 expression occurred 1 h after DcMYB1 expression in all stress treatments, and repression of DcMYB1 expression by RNA interference caused cessation of the up-regulation of DcPAL1 expression in the elicitor treatment or with UV-B irradiation. These results suggest that DcMYB1 is the main regulatory factor acting on box-L sequences in the DcPAL1 gene that respond to environmental cues.

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Abbreviations

At :

Arabidopsis thaliana

Dc :

Daucus carota

DsRNA:

double-stranded RNA

LTR:

long-terminal repeat

luc :

firefly luciferase

MBS:

myb binding site

Nt :

Nicotiana tabacum

PAL:

phenylalanine ammonia-lyase

RNAi:

double-stranded RNA interference

UV:

ultraviolet

References

  • J.O. Borevitz Y. Xia J. Blount R.A. Dixon C. Lamb (2000) ArticleTitleActivation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis Plant Cell 12 2383–2394 Occurrence Handle10.1105/tpc.12.12.2383 Occurrence Handle11148285

    Article  PubMed  Google Scholar 

  • Y.H. Cheong H.S. Chang R. Gupta X. Wang T. Zhu S. Luan (2002) ArticleTitleTranscriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis Plant Physiol. 129 661–677 Occurrence Handle10.1104/pp.002857 Occurrence Handle12068110

    Article  PubMed  Google Scholar 

  • R.A. Dixon (2001) ArticleTitleNatural products and plant disease resistance Nature 411 843–847 Occurrence Handle10.1038/35081178 Occurrence Handle11459067

    Article  PubMed  Google Scholar 

  • R.A. Dixon N.L. Paiva (1995) ArticleTitleStress-induced phenylpropanoid metabolism Plant Cell 7 1085–1097 Occurrence Handle10.1105/tpc.7.7.1085 Occurrence Handle12242399

    Article  PubMed  Google Scholar 

  • T. Fukasawa-Akada S.D. Kung J.C. Watson (1996) ArticleTitlePhenylalanine ammonia-lyase gene structure, expression, and evolution in Nicotiana Plant Mol. Biol. 30 711–722 Occurrence Handle10.1007/BF00019006 Occurrence Handle8624404

    Article  PubMed  Google Scholar 

  • E. Grotewold B.J. Drummond B. Bowen T. Peterson (1994) ArticleTitleThe myb-homologous P gene controls phlobaphene pigmentation in maize floral organs by directly activating a flavonoid biosynthetic gene subset Cell 76 543–553 Occurrence Handle10.1016/0092-8674(94)90117-1 Occurrence Handle8313474

    Article  PubMed  Google Scholar 

  • E. Grotewold M.B. Sainz L. Tagliani J.M. Hernandez B. Bowen V.L. Chandler (2000) ArticleTitleIdentification of the residues in the Myb domain of maize C1 that specify the interaction with the bHLH cofactor R Proc. Natl. Acad. Sci. USA 97 13579–13584 Occurrence Handle10.1073/pnas.250379897 Occurrence Handle11095727

    Article  PubMed  Google Scholar 

  • H. Jin E. Cominelli P. Bailey A. Parr F. Mehrtens J. Jones C. Tonelli B. Weisshaar C. Martin (2000) ArticleTitleTranscriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis EMBO J. 19 6150–6161 Occurrence Handle10.1093/emboj/19.22.6150 Occurrence Handle11080161

    Article  PubMed  Google Scholar 

  • L.G. Landry C.C. Chapple R.L. Last (1995) ArticleTitleArabidopsis mutants lacking phenolic sunscreens exhibit enhanced ultraviolet-B injury and oxidative damage Plant Physiol. 109 1159–1166 Occurrence Handle10.1104/pp.109.4.1159 Occurrence Handle8539286

    Article  PubMed  Google Scholar 

  • E. Logemann M. Parniske K. Hahlbrock (1995) ArticleTitleModes of expression and common structural features of the complete phenylalanine ammonia-lyase gene family in parsley Proc. Natl. Acad. Sci. USA 92 5905–5909 Occurrence Handle7597051

    PubMed  Google Scholar 

  • R. Lois A. Dietrich K. Hahlbrock W. Schulz (1989) ArticleTitleA phenylalanine ammonia-lyase gene from parsley: structure, regulation and identification of elicitor and light responsive cis-acting elements EMBO J. 8 1641–1648 Occurrence Handle2767049

    PubMed  Google Scholar 

  • J.C. Long G.I. Jenkins (1998) ArticleTitleInvolvement of plasma membrane redox activity and calcium homeostasis in the UV-B and UV-A/blue light induction of gene expression in Arabidopsis Plant Cell 10 2077–2086 Occurrence Handle10.1105/tpc.10.12.2077 Occurrence Handle9836746

    Article  PubMed  Google Scholar 

  • Y. Ozeki A. Komamine (1981) ArticleTitleInduction of anthocyanin synthesis in relation to embryogenesis in carrot suspension culture: correlation of metabolic differentiation with morphological differentiation Plant Physiol. 53 570–577

    Google Scholar 

  • Y. Ozeki E. Davies J. Takeda (1993) ArticleTitleStructure and expression of the chalcone synthase gene in carrot suspension cultured cells regulated by 2,4-D Plant Cell Physiol. 34 1029–1037

    Google Scholar 

  • Y. Ozeki M. Matui M. Sakuta M. Matsuoka Y. Ohasi Y. Kano-Murakami N. Yamamoto Y. Tanaka (1990) ArticleTitleDifferential regulation of phenylalanine ammonia-lyase genes during anthocyanin synthesis and by transfer effect in carrot cell suspension cultures Physiol. Plant. 80 379–387 Occurrence Handle10.1034/j.1399-3054.1990.800308.x

    Article  Google Scholar 

  • I. Romero A. Fuertes M.J. Benito J.M. Malpica A. Leyva J. Paz-Ares (1998) ArticleTitleMore than 80R2R3-MYB regulatory genes in the genome of Arabidopsis thaliana Plant J. 14 273–284 Occurrence Handle10.1046/j.1365-313X.1998.00113.x Occurrence Handle9628022

    Article  PubMed  Google Scholar 

  • D. Shufflebottom K. Edwards W. Schuch M. Bevan (1993) ArticleTitleTranscription of two members of a gene family encoding phenylalanine ammonia-lyase leads to remarkably different cell specificities and induction patterns Plant J. 3 835–845 Occurrence Handle10.1111/j.1365-313X.1993.00835.x Occurrence Handle8401614

    Article  PubMed  Google Scholar 

  • K. Sugimoto S. Takeda H. Hirochika (2000) ArticleTitleMYB-related transcription factor NtMYB2 induced by wounding and elicitors is a regulator of the tobacco retrotransposon Tto1 and defense-related genes Plant Cell 12 2511–2528 Occurrence Handle10.1105/tpc.12.12.2511 Occurrence Handle11148294

    Article  PubMed  Google Scholar 

  • J. Takeda Y. Ito K. Maeda Y. Ozeki (2002) ArticleTitleAssignment of UVB-responsive cis-element and protoplastization-(dilution-) and elicitor-responsive ones in the promoter region of a carrot phenylalanine ammonia-lyase gene (gDcPAL1) Photochem. Photobiol. 76 232–238 Occurrence Handle10.1562/0031-8655(2002)076<0232:AOURCE>2.0.CO;2 Occurrence Handle12194222

    Article  PubMed  Google Scholar 

  • J. Takeda Y. Ozeki K. Yoshida (1997) ArticleTitleAction spectrum for induction of promoter activity of phenylalanine ammonia-lyase gene by UV in carrot suspension cells Photochem. Photobiol. 66 464–470 Occurrence Handle9337617

    PubMed  Google Scholar 

  • M. Takeuchi T. Ueda K. Sato H. Abe T. Nagata A. Nakano (2000) ArticleTitleA dominant negative mutant of sar1 GTPase inhibits protein transport from the endoplasmic reticulum to the Golgi apparatus in tobacco and Arabidopsis cultured cells Plant J. 23 517–525 Occurrence Handle10.1046/j.1365-313x.2000.00823.x Occurrence Handle10972878

    Article  PubMed  Google Scholar 

  • L.A. Wanner G. Li D. Ware I.E. Somssich K.R. Davis (1995) ArticleTitleThe phenylalanine ammonia-lyase gene family in Arabidopsis thaliana Plant Mol. Biol. 27 327–338 Occurrence Handle10.1007/BF00020187 Occurrence Handle7888622

    Article  PubMed  Google Scholar 

  • R. Wingender H. Rohrig C. Horicke J. Schell (1990) ArticleTitleCis-regulatory elements involved in ultraviolet light regulation and plant defense Plant Cell 2 1019–1026 Occurrence Handle10.1105/tpc.2.10.1019 Occurrence Handle2136625

    Article  PubMed  Google Scholar 

  • S. Yang J.P. Sweetman S. Amirsadeghi M. Barghchi A.K. Huttly W.I. Chung D. Twell (2001) ArticleTitleNovel anther-specific myb genes from tobacco as putative regulators of phenylalanine ammonia-lyase expression Plant Physiol. 126 1738–1753 Occurrence Handle10.1104/pp.126.4.1738 Occurrence Handle11500571

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, 184-8588, Koganei, Tokyo, Japan

    Kazuhiro Maeda, Soichi Kimura & Yoshihiro Ozeki

  2. Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, 230-0045, Yokohama, Kanagawa, Japan

    Taku Demura

  3. Department of Faculty of Human Life and Environment, , Nara Woman’s University, 630-8506, Kitauoyahigashi-machi, Nara, Japan

    Junko Takeda

Authors
  1. Kazuhiro Maeda
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  2. Soichi Kimura
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  3. Taku Demura
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  4. Junko Takeda
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  5. Yoshihiro Ozeki
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Correspondence to Yoshihiro Ozeki.

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Maeda, K., Kimura, S., Demura, T. et al. DcMYB1 Acts as a Transcriptional Activator of the Carrot Phenylalanine Ammonia-lyase Gene (DcPAL1) in Response to Elicitor Treatment, UV-B Irradiation and the Dilution Effect. Plant Mol Biol 59, 739–752 (2005). https://doi.org/10.1007/s11103-005-0910-6

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  • DOI: https://doi.org/10.1007/s11103-005-0910-6

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